Next Generation Sequencing

Library Preparation for Next Generation Sequencing

NEBNext® reagents are a series of highly pure reagents that facilitate sample preparation of DNA or RNA for next generation sequencing, and target enrichment of DNA. Available for a wide range of sample types, NEBNext products are provided in user-friendly formats including kits and modules, with bulk or customized formats also available. Adaptors and primers are available separately, for maximum flexibility. These robust reagents undergo stringent quality controls and functional validation, ensuring maximum yield, convenience and value.

Use the following tool to determine the best NEBNext products for you:






NEBNext® Sample Preparation for Next Generation Sequencing

Close

OrderNEBNext Direct® BRCA1/BRCA2 Panel

Close

OrderNEBNext Direct® Cancer HotSpot Panel

Close

OrderNEBNext® Multiplex Oligos for Illumina® (Index Primers Set 3)

Close

OrderNEBNext® Multiplex Small RNA Library Prep Kit for Illumina® (Index Primers 1-48)

Close

OrderNEBNext® Ultra™ II Ligation Module 

Close

OrderNEBNext® Ultra™ II Q5® Master Mix

Close

OrderNEBNext® Ultra™ II End Repair/dA-Tailing Module

Close

OrderNEBNext® Multiplex Oligos for Illumina® (96 Index Primers)

Close

OrderNEBNext® Multiplex Oligos for Illumina® (Index Primers Set 4)

Close

OrderNEBNext® Ultra™ II DNA Library Prep Kit for Illumina®

Close

OrderNEBNext® Ultra II Directional RNA Library Prep Kit for Illumina®

Close

OrderNEBNext® Ultra II Directional RNA Library Prep with Sample Purification Beads

Close

OrderNEBNext® Ultra II DNA Library Prep with Sample Purification Beads

Close

OrderNEBNext® Ultra II RNA First Strand Synthesis Module

Close

OrderNEBNext® Ultra II RNA Library Prep Kit for Illumina®

Close

OrderNEBNext® Ultra II RNA Library Prep with Sample Purification Beads

Close

OrderNuclease-free Water

Illumina® Library Preparation

Close

OrderNEBNext Direct® BRCA1/BRCA2 Panel

Close

OrderNEBNext Direct® Cancer HotSpot Panel

Close

OrderNEBNext® Multiplex Oligos for Illumina® (Index Primers Set 3)

Close

OrderNEBNext® Multiplex Small RNA Library Prep Kit for Illumina® (Index Primers 1-48)

Close

OrderNEBNext® ChIP-Seq Library Prep Master Mix Set for Illumina®

Close

OrderNEBNext® ChIP-Seq Library Prep Reagent Set for Illumina®

Close

OrderNEBNext® dA-Tailing Module

Close

OrderNEBNext® DNA Library Prep Master Mix Set for Illumina®

Close

NEBNext® DNA Library Prep Reagent Set for Illumina®

Close

OrderNEBNext® End Repair Module

Close

OrderNEBNext® Magnesium RNA Fragmentation Module

Close

NEBNext® mRNA Library Prep Master Mix Set for Illumina®

Close

NEBNext® mRNA Library Prep Reagent Set for Illumina®

Close

OrderNEBNext® Multiplex Oligos for Illumina® (Index Primers Set 1)

Close

OrderNEBNext® Multiplex Oligos for Illumina® (Index Primers Set 2)

Close

OrderNEBNext® Multiplex Oligos for Illumina® (Methylated Adaptor, Index Primers Set 1)

Close

OrderNEBNext® Multiplex Small RNA Library Prep Set for Illumina® (Set 2)

Close

OrderNEBNext® Multiplex Oligos for Illumina® (Dual Index Primers Set 1)

Close

OrderNEBNext® Poly(A) mRNA Magnetic Isolation Module

Close

OrderNEBNext® Q5® Hot Start HiFi PCR Master Mix

Close

OrderNEBNext® Quick Ligation Module

Close

OrderNEBNext® RNA First Strand Synthesis Module

Close

OrderNEBNext® Singleplex Oligos for Illumina®

Close

OrderNEBNext® Small RNA Library Prep Set for Illumina® (Multiplex Compatible)

Close

OrderNEBNext® Ultra™ End Repair/dA-Tailing Module

Close

OrderNEBNext® Ultra™ II Directional RNA Second Strand Synthesis Module

Close

OrderNEBNext® Ultra II Non-Directional RNA Second Strand Synthesis Module

Close

OrderNEBNext® Ultra™ Ligation Module

Close

OrderNEBNext® Ultra™ II Ligation Module 

Close

OrderNEBNext® Ultra™ II Q5® Master Mix

Close

OrderNEBNext® Ultra™ II End Repair/dA-Tailing Module

Close

OrderNEBNext® Ultra™ DNA Library Prep Kit for Illumina®

Close

OrderNEBNext® Library Dilution Buffer

Close

OrderNEBNext® FFPE DNA Repair Mix

Close

OrderNEBNext® Multiplex Oligos for Illumina® (96 Index Primers)

Close

OrderNEBNext® Multiplex Oligos for Illumina® (Index Primers Set 4)

Close

OrderNEBNext® Multiplex Small RNA Library Prep Set for Illumina® (Set 1)

Close

OrderNEBNext® rRNA Depletion Kit (Human/Mouse/Rat)

Close

OrderNEBNext® Ultra™ II DNA Library Prep Kit for Illumina®

Close

OrderNEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina®

Close

OrderNEBNext® Ultra™ RNA Library Prep Kit for Illumina®

Close

OrderNEBNext® Ultra II Directional RNA Library Prep Kit for Illumina®

Close

OrderNEBNext® Ultra II Directional RNA Library Prep with Sample Purification Beads

Close

OrderNEBNext® Ultra II DNA Library Prep with Sample Purification Beads

Close

OrderNEBNext® Ultra II RNA First Strand Synthesis Module

Close

OrderNEBNext® Ultra II RNA Library Prep Kit for Illumina®

Close

OrderNEBNext® Ultra II RNA Library Prep with Sample Purification Beads

Close

OrderNEBNext® Library Quant Kit for Illumina®

Ion Torrent™ DNA Library Preparation

Close

OrderNEBNext® Fast DNA Fragmentation & Library Prep Set for Ion Torrent™

Close

OrderNEBNext® Fast DNA Library Prep Set for Ion Torrent™

Close

OrderNEBNext® Q5® Hot Start HiFi PCR Master Mix

Close

OrderNEBNext® FFPE DNA Repair Mix

454™ Library Preparation

Close

OrderNEBNext® DNA Library Prep Master Mix Set for 454™

Close

OrderNEBNext® End Repair Module

Close

OrderNEBNext® Magnesium RNA Fragmentation Module

Close

OrderNEBNext® Poly(A) mRNA Magnetic Isolation Module

Close

OrderNEBNext® Quick Ligation Module

Close

OrderNEBNext® Ultra II Non-Directional RNA Second Strand Synthesis Module

DNA Library Preparation

Close

OrderNEBNext® High-Fidelity 2X PCR Master Mix

Close

OrderNEBNext Direct® BRCA1/BRCA2 Panel

Close

OrderNEBNext Direct® Cancer HotSpot Panel

Close

OrderNEBNext® Multiplex Oligos for Illumina® (Index Primers Set 3)

Close

OrderNEBNext® dA-Tailing Reaction Buffer

Close

OrderNEBNext® End Repair Reaction Buffer

Close

OrderNEBNext® Q5® Hot Start HiFi PCR Master Mix

Close

OrderNEBNext® Quick Ligation Reaction Buffer

Close

OrderNEBNext® Ultra™ II Ligation Module 

Close

OrderNEBNext® Ultra™ II Q5® Master Mix

Close

OrderNEBNext® Ultra™ II End Repair/dA-Tailing Module

Close

OrderNEBNext® Library Dilution Buffer

Close

OrderNEBNext® FFPE DNA Repair Mix

Close

OrderNEBNext® Multiplex Oligos for Illumina® (96 Index Primers)

Close

OrderNEBNext® Multiplex Oligos for Illumina® (Index Primers Set 4)

Close

OrderNEBNext® Ultra™ II DNA Library Prep Kit for Illumina®

Close

OrderNEBNext® Ultra II DNA Library Prep with Sample Purification Beads

Close

OrderNEBNext® Library Quant Kit for Illumina®

RNA Library Preparation

Close

Order5´ DNA Adenylation Kit

Close

OrderNEBNext® Multiplex Small RNA Library Prep Kit for Illumina® (Index Primers 1-48)

Close

OrderNEBNext® Q5® Hot Start HiFi PCR Master Mix

Close

OrderNEBNext® Second Strand Synthesis (dNTP-free) Reaction Buffer

Close

OrderNEBNext® Library Dilution Buffer

Close

OrderNEBNext® rRNA Depletion Kit (Human/Mouse/Rat)

Close

OrderNEBNext® Ultra II Directional RNA Library Prep Kit for Illumina®

Close

OrderNEBNext® Ultra II Directional RNA Library Prep with Sample Purification Beads

Close

OrderNEBNext® Ultra II RNA First Strand Synthesis Module

Close

OrderNEBNext® Ultra II RNA Library Prep Kit for Illumina®

Close

OrderNEBNext® Ultra II RNA Library Prep with Sample Purification Beads

Close

OrderNEBNext® Library Quant Kit for Illumina®

DNA Fragmentation

Close

OrderNEBNext® dsDNA Fragmentase® Reaction Buffer

Close

OrderNEBNext® dsDNA Fragmentase®

Close

OrderNEBNext® dsDNA Fragmentase® Reaction Buffer v2

Close

OrderNEBNext® Fast DNA Fragmentation & Library Prep Set for Ion Torrent™

NEBNext® Automation

FFPE DNA Repair

Illumina® Library Preparation

Close

OrderNEBNext Direct® BRCA1/BRCA2 Panel

Close

OrderNEBNext Direct® Cancer HotSpot Panel

Close

OrderNEBNext® Multiplex Oligos for Illumina® (Index Primers Set 3)

Close

OrderNEBNext® Multiplex Small RNA Library Prep Kit for Illumina® (Index Primers 1-48)

Close

OrderNEBNext® ChIP-Seq Library Prep Master Mix Set for Illumina®

Close

OrderNEBNext® ChIP-Seq Library Prep Reagent Set for Illumina®

Close

OrderNEBNext® dA-Tailing Module

Close

OrderNEBNext® DNA Library Prep Master Mix Set for Illumina®

Close

NEBNext® DNA Library Prep Reagent Set for Illumina®

Close

OrderNEBNext® End Repair Module

Close

OrderNEBNext® Magnesium RNA Fragmentation Module

Close

NEBNext® mRNA Library Prep Master Mix Set for Illumina®

Close

NEBNext® mRNA Library Prep Reagent Set for Illumina®

Close

OrderNEBNext® Multiplex Oligos for Illumina® (Index Primers Set 1)

Close

OrderNEBNext® Multiplex Oligos for Illumina® (Index Primers Set 2)

Close

OrderNEBNext® Multiplex Oligos for Illumina® (Methylated Adaptor, Index Primers Set 1)

Close

OrderNEBNext® Multiplex Small RNA Library Prep Set for Illumina® (Set 2)

Close

OrderNEBNext® Multiplex Oligos for Illumina® (Dual Index Primers Set 1)

Close

OrderNEBNext® Poly(A) mRNA Magnetic Isolation Module

Close

OrderNEBNext® Q5® Hot Start HiFi PCR Master Mix

Close

OrderNEBNext® Quick Ligation Module

Close

OrderNEBNext® RNA First Strand Synthesis Module

Close

OrderNEBNext® Singleplex Oligos for Illumina®

Close

OrderNEBNext® Small RNA Library Prep Set for Illumina® (Multiplex Compatible)

Close

OrderNEBNext® Ultra™ End Repair/dA-Tailing Module

Close

OrderNEBNext® Ultra™ II Directional RNA Second Strand Synthesis Module

Close

OrderNEBNext® Ultra II Non-Directional RNA Second Strand Synthesis Module

Close

OrderNEBNext® Ultra™ Ligation Module

Close

OrderNEBNext® Ultra™ II Ligation Module 

Close

OrderNEBNext® Ultra™ II Q5® Master Mix

Close

OrderNEBNext® Ultra™ II End Repair/dA-Tailing Module

Close

OrderNEBNext® Ultra™ DNA Library Prep Kit for Illumina®

Close

OrderNEBNext® Library Dilution Buffer

Close

OrderNEBNext® FFPE DNA Repair Mix

Close

OrderNEBNext® Multiplex Oligos for Illumina® (96 Index Primers)

Close

OrderNEBNext® Multiplex Oligos for Illumina® (Index Primers Set 4)

Close

OrderNEBNext® Multiplex Small RNA Library Prep Set for Illumina® (Set 1)

Close

OrderNEBNext® rRNA Depletion Kit (Human/Mouse/Rat)

Close

OrderNEBNext® Ultra™ II DNA Library Prep Kit for Illumina®

Close

OrderNEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina®

Close

OrderNEBNext® Ultra™ RNA Library Prep Kit for Illumina®

Close

OrderNEBNext® Ultra II Directional RNA Library Prep Kit for Illumina®

Close

OrderNEBNext® Ultra II Directional RNA Library Prep with Sample Purification Beads

Close

OrderNEBNext® Ultra II DNA Library Prep with Sample Purification Beads

Close

OrderNEBNext® Ultra II RNA First Strand Synthesis Module

Close

OrderNEBNext® Ultra II RNA Library Prep Kit for Illumina®

Close

OrderNEBNext® Ultra II RNA Library Prep with Sample Purification Beads

Close

OrderNEBNext® Library Quant Kit for Illumina®

NEBNext® Ultra™ II DNA Library Prep

Close

OrderNEBNext® Ultra™ II Ligation Module 

Close

OrderNEBNext® Ultra™ II End Repair/dA-Tailing Module

Close

OrderNEBNext® Ultra™ II DNA Library Prep Kit for Illumina®

Close

OrderNEBNext® Ultra II DNA Library Prep with Sample Purification Beads

NEBNext® Ultra™ II RNA Library Prep

DNA for Illumina®

Close

OrderNEBNext® ChIP-Seq Library Prep Master Mix Set for Illumina®

Close

OrderNEBNext® ChIP-Seq Library Prep Reagent Set for Illumina®

Close

OrderNEBNext® dA-Tailing Module

Close

OrderNEBNext® DNA Library Prep Master Mix Set for Illumina®

Close

NEBNext® DNA Library Prep Reagent Set for Illumina®

Close

OrderNEBNext® End Repair Module

Close

OrderNEBNext® Multiplex Oligos for Illumina® (Index Primers Set 2)

Close

OrderNEBNext® Multiplex Oligos for Illumina® (Dual Index Primers Set 1)

Close

OrderNEBNext® Quick Ligation Module

Close

OrderNEBNext® Ultra™ End Repair/dA-Tailing Module

Close

OrderNEBNext® Ultra™ Ligation Module

Close

OrderNEBNext® Ultra™ DNA Library Prep Kit for Illumina®

RNA for Illumina®

Close

OrderNEBNext® Magnesium RNA Fragmentation Module

Close

NEBNext® mRNA Library Prep Master Mix Set for Illumina®

Close

NEBNext® mRNA Library Prep Reagent Set for Illumina®

Close

OrderNEBNext® Multiplex Oligos for Illumina® (Index Primers Set 1)

Close

OrderNEBNext® Multiplex Oligos for Illumina® (Methylated Adaptor, Index Primers Set 1)

Close

OrderNEBNext® Multiplex Small RNA Library Prep Set for Illumina® (Set 2)

Close

OrderNEBNext® Poly(A) mRNA Magnetic Isolation Module

Close

OrderNEBNext® RNA First Strand Synthesis Module

Close

OrderNEBNext® Singleplex Oligos for Illumina®

Close

OrderNEBNext® Small RNA Library Prep Set for Illumina® (Multiplex Compatible)

Close

OrderNEBNext® Ultra™ II Directional RNA Second Strand Synthesis Module

Close

OrderNEBNext® Ultra II Non-Directional RNA Second Strand Synthesis Module

Close

OrderNEBNext® Multiplex Small RNA Library Prep Set for Illumina® (Set 1)

Close

OrderNEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina®

Close

OrderNEBNext® Ultra™ RNA Library Prep Kit for Illumina®

Close

OrderNEBNext® Ultra II RNA First Strand Synthesis Module

Close

OrderNEBNext® Ultra II RNA Library Prep Kit for Illumina®

Ion Torrent™ DNA Library Preparation

Close

OrderNEBNext® Fast DNA Fragmentation & Library Prep Set for Ion Torrent™

Close

OrderNEBNext® Fast DNA Library Prep Set for Ion Torrent™

Close

OrderNEBNext® Q5® Hot Start HiFi PCR Master Mix

Close

OrderNEBNext® FFPE DNA Repair Mix

DNA for Ion Torrent™

Close

OrderNEBNext® Fast DNA Fragmentation & Library Prep Set for Ion Torrent™

Close

OrderNEBNext® Fast DNA Library Prep Set for Ion Torrent™

454™ Library Preparation

Close

OrderNEBNext® DNA Library Prep Master Mix Set for 454™

Close

OrderNEBNext® End Repair Module

Close

OrderNEBNext® Magnesium RNA Fragmentation Module

Close

OrderNEBNext® Poly(A) mRNA Magnetic Isolation Module

Close

OrderNEBNext® Quick Ligation Module

Close

OrderNEBNext® Ultra II Non-Directional RNA Second Strand Synthesis Module

DNA for 454™

Close

OrderNEBNext® DNA Library Prep Master Mix Set for 454™

Close

OrderNEBNext® End Repair Module

Close

OrderNEBNext® Quick Ligation Module

RNA for 454™

Close

OrderNEBNext® Magnesium RNA Fragmentation Module

Close

OrderNEBNext® Poly(A) mRNA Magnetic Isolation Module

Close

OrderNEBNext® Ultra II Non-Directional RNA Second Strand Synthesis Module

DNA Library Preparation

Close

OrderNEBNext® High-Fidelity 2X PCR Master Mix

Close

OrderNEBNext Direct® BRCA1/BRCA2 Panel

Close

OrderNEBNext Direct® Cancer HotSpot Panel

Close

OrderNEBNext® Multiplex Oligos for Illumina® (Index Primers Set 3)

Close

OrderNEBNext® dA-Tailing Reaction Buffer

Close

OrderNEBNext® End Repair Reaction Buffer

Close

OrderNEBNext® Q5® Hot Start HiFi PCR Master Mix

Close

OrderNEBNext® Quick Ligation Reaction Buffer

Close

OrderNEBNext® Ultra™ II Ligation Module 

Close

OrderNEBNext® Ultra™ II Q5® Master Mix

Close

OrderNEBNext® Ultra™ II End Repair/dA-Tailing Module

Close

OrderNEBNext® Library Dilution Buffer

Close

OrderNEBNext® FFPE DNA Repair Mix

Close

OrderNEBNext® Multiplex Oligos for Illumina® (96 Index Primers)

Close

OrderNEBNext® Multiplex Oligos for Illumina® (Index Primers Set 4)

Close

OrderNEBNext® Ultra™ II DNA Library Prep Kit for Illumina®

Close

OrderNEBNext® Ultra II DNA Library Prep with Sample Purification Beads

Close

OrderNEBNext® Library Quant Kit for Illumina®

RNA Library Preparation

Close

Order5´ DNA Adenylation Kit

Close

OrderNEBNext® Multiplex Small RNA Library Prep Kit for Illumina® (Index Primers 1-48)

Close

OrderNEBNext® Q5® Hot Start HiFi PCR Master Mix

Close

OrderNEBNext® Second Strand Synthesis (dNTP-free) Reaction Buffer

Close

OrderNEBNext® Library Dilution Buffer

Close

OrderNEBNext® rRNA Depletion Kit (Human/Mouse/Rat)

Close

OrderNEBNext® Ultra II Directional RNA Library Prep Kit for Illumina®

Close

OrderNEBNext® Ultra II Directional RNA Library Prep with Sample Purification Beads

Close

OrderNEBNext® Ultra II RNA First Strand Synthesis Module

Close

OrderNEBNext® Ultra II RNA Library Prep Kit for Illumina®

Close

OrderNEBNext® Ultra II RNA Library Prep with Sample Purification Beads

Close

OrderNEBNext® Library Quant Kit for Illumina®

DNA Fragmentation

Close

OrderNEBNext® Fast DNA Fragmentation & Library Prep Set for Ion Torrent™

NEBNext® dsDNA Fragmentase®

Close

OrderNEBNext® dsDNA Fragmentase® Reaction Buffer

Close

OrderNEBNext® dsDNA Fragmentase®

Close

OrderNEBNext® dsDNA Fragmentase® Reaction Buffer v2

NEBNext® Automation

FFPE DNA Repair

NEBNext FFPE DNA Repair Mix

Can't find what you need?