Next Generation Sequencing

Library Preparation for Next Generation Sequencing

NEBNext® reagents are a series of highly pure reagents that facilitate sample preparation of DNA or RNA for next generation sequencing, and target enrichment of DNA. Available for a wide range of sample types, NEBNext products are provided in user-friendly formats including kits and modules, with bulk or customized formats also available. Adaptors and primers are available separately, for maximum flexibility. These robust reagents undergo stringent quality controls and functional validation, ensuring maximum yield, convenience and value.

Use the following tool to determine the best NEBNext products for you:






NEBNext® Sample Preparation for Next Generation Sequencing

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NEBNext Direct® BRCA1/BRCA2 Panel

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NEBNext Direct® Cancer HotSpot Panel

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NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 3)

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NEBNext® Multiplex Small RNA Library Prep Kit for Illumina® (Index Primers 1-48)

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NEBNext® Ultra™ II Ligation Module 

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NEBNext® Ultra™ II Q5® Master Mix

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NEBNext® Ultra™ II End Repair/dA-Tailing Module

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NEBNext® Multiplex Oligos for Illumina® (96 Index Primers)

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NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 4)

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NEBNext® Ultra™ II DNA Library Prep Kit for Illumina®

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NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina®

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NEBNext® Ultra II Directional RNA Library Prep with Sample Purification Beads

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NEBNext® Ultra II DNA Library Prep with Sample Purification Beads

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NEBNext® Ultra II RNA First Strand Synthesis Module

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NEBNext® Ultra II RNA Library Prep Kit for Illumina®

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NEBNext® Ultra II RNA Library Prep with Sample Purification Beads

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Nuclease-free Water

Illumina® Library Preparation

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NEBNext Direct® BRCA1/BRCA2 Panel

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NEBNext Direct® Cancer HotSpot Panel

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NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 3)

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NEBNext® Multiplex Small RNA Library Prep Kit for Illumina® (Index Primers 1-48)

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NEBNext® ChIP-Seq Library Prep Master Mix Set for Illumina®

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NEBNext® ChIP-Seq Library Prep Reagent Set for Illumina®

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NEBNext® dA-Tailing Module

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NEBNext® DNA Library Prep Master Mix Set for Illumina®

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NEBNext® DNA Library Prep Reagent Set for Illumina®

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NEBNext® End Repair Module

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NEBNext® Magnesium RNA Fragmentation Module

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NEBNext® mRNA Library Prep Master Mix Set for Illumina®

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NEBNext® mRNA Library Prep Reagent Set for Illumina®

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NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 1)

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NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 2)

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NEBNext® Multiplex Oligos for Illumina® (Methylated Adaptor, Index Primers Set 1)

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NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (Set 2)

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NEBNext® Multiplex Oligos for Illumina® (Dual Index Primers Set 1)

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NEBNext® Poly(A) mRNA Magnetic Isolation Module

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NEBNext® Q5® Hot Start HiFi PCR Master Mix

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NEBNext® Quick Ligation Module

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NEBNext® RNA First Strand Synthesis Module

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NEBNext® Singleplex Oligos for Illumina®

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NEBNext® Small RNA Library Prep Set for Illumina® (Multiplex Compatible)

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NEBNext® Ultra™ End Repair/dA-Tailing Module

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NEBNext® Ultra™ II Directional RNA Second Strand Synthesis Module

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NEBNext® Ultra II Non-Directional RNA Second Strand Synthesis Module

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NEBNext® Ultra™ Ligation Module

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NEBNext® Ultra™ II Ligation Module 

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NEBNext® Ultra™ II Q5® Master Mix

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NEBNext® Ultra™ II End Repair/dA-Tailing Module

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NEBNext® Ultra™ DNA Library Prep Kit for Illumina®

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NEBNext® Library Dilution Buffer

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NEBNext® FFPE DNA Repair Mix

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NEBNext® Multiplex Oligos for Illumina® (96 Index Primers)

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NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 4)

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NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (Set 1)

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NEBNext® rRNA Depletion Kit (Human/Mouse/Rat)

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NEBNext® Ultra™ II DNA Library Prep Kit for Illumina®

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NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina®

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NEBNext® Ultra™ RNA Library Prep Kit for Illumina®

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NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina®

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NEBNext® Ultra II Directional RNA Library Prep with Sample Purification Beads

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NEBNext® Ultra II DNA Library Prep with Sample Purification Beads

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NEBNext® Ultra II RNA First Strand Synthesis Module

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NEBNext® Ultra II RNA Library Prep Kit for Illumina®

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NEBNext® Ultra II RNA Library Prep with Sample Purification Beads

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NEBNext® Library Quant Kit for Illumina®

Ion Torrent™ DNA Library Preparation

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NEBNext® Fast DNA Fragmentation & Library Prep Set for Ion Torrent™

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NEBNext® Fast DNA Library Prep Set for Ion Torrent™

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NEBNext® Q5® Hot Start HiFi PCR Master Mix

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NEBNext® FFPE DNA Repair Mix

454™ Library Preparation

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NEBNext® DNA Library Prep Master Mix Set for 454™

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NEBNext® End Repair Module

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NEBNext® Magnesium RNA Fragmentation Module

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NEBNext® Poly(A) mRNA Magnetic Isolation Module

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NEBNext® Quick Ligation Module

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NEBNext® Ultra II Non-Directional RNA Second Strand Synthesis Module

DNA Library Preparation

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NEBNext® High-Fidelity 2X PCR Master Mix

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NEBNext Direct® BRCA1/BRCA2 Panel

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NEBNext Direct® Cancer HotSpot Panel

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NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 3)

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NEBNext® dA-Tailing Reaction Buffer

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NEBNext® End Repair Reaction Buffer

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NEBNext® Q5® Hot Start HiFi PCR Master Mix

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NEBNext® Quick Ligation Reaction Buffer

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NEBNext® Ultra™ II Ligation Module 

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NEBNext® Ultra™ II Q5® Master Mix

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NEBNext® Ultra™ II End Repair/dA-Tailing Module

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NEBNext® Library Dilution Buffer

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NEBNext® FFPE DNA Repair Mix

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NEBNext® Multiplex Oligos for Illumina® (96 Index Primers)

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NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 4)

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NEBNext® Ultra™ II DNA Library Prep Kit for Illumina®

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NEBNext® Ultra II DNA Library Prep with Sample Purification Beads

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NEBNext® Library Quant Kit for Illumina®

RNA Library Preparation

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5´ DNA Adenylation Kit

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NEBNext® Multiplex Small RNA Library Prep Kit for Illumina® (Index Primers 1-48)

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NEBNext® Q5® Hot Start HiFi PCR Master Mix

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NEBNext® Second Strand Synthesis (dNTP-free) Reaction Buffer

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NEBNext® Library Dilution Buffer

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NEBNext® rRNA Depletion Kit (Human/Mouse/Rat)

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NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina®

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NEBNext® Ultra II Directional RNA Library Prep with Sample Purification Beads

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NEBNext® Ultra II RNA First Strand Synthesis Module

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NEBNext® Ultra II RNA Library Prep Kit for Illumina®

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NEBNext® Ultra II RNA Library Prep with Sample Purification Beads

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NEBNext® Library Quant Kit for Illumina®

DNA Fragmentation

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NEBNext® dsDNA Fragmentase® Reaction Buffer

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NEBNext® dsDNA Fragmentase®

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NEBNext® dsDNA Fragmentase® Reaction Buffer v2

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NEBNext® Fast DNA Fragmentation & Library Prep Set for Ion Torrent™

NEBNext® Automation

FFPE DNA Repair

Illumina® Library Preparation

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NEBNext Direct® BRCA1/BRCA2 Panel

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NEBNext Direct® Cancer HotSpot Panel

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NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 3)

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NEBNext® Multiplex Small RNA Library Prep Kit for Illumina® (Index Primers 1-48)

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NEBNext® ChIP-Seq Library Prep Master Mix Set for Illumina®

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NEBNext® ChIP-Seq Library Prep Reagent Set for Illumina®

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NEBNext® dA-Tailing Module

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NEBNext® DNA Library Prep Master Mix Set for Illumina®

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NEBNext® DNA Library Prep Reagent Set for Illumina®

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NEBNext® End Repair Module

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NEBNext® Magnesium RNA Fragmentation Module

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NEBNext® mRNA Library Prep Master Mix Set for Illumina®

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NEBNext® mRNA Library Prep Reagent Set for Illumina®

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NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 1)

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NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 2)

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NEBNext® Multiplex Oligos for Illumina® (Methylated Adaptor, Index Primers Set 1)

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NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (Set 2)

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NEBNext® Multiplex Oligos for Illumina® (Dual Index Primers Set 1)

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NEBNext® Poly(A) mRNA Magnetic Isolation Module

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NEBNext® Q5® Hot Start HiFi PCR Master Mix

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NEBNext® Quick Ligation Module

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NEBNext® RNA First Strand Synthesis Module

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NEBNext® Singleplex Oligos for Illumina®

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NEBNext® Small RNA Library Prep Set for Illumina® (Multiplex Compatible)

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NEBNext® Ultra™ End Repair/dA-Tailing Module

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NEBNext® Ultra™ II Directional RNA Second Strand Synthesis Module

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NEBNext® Ultra II Non-Directional RNA Second Strand Synthesis Module

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NEBNext® Ultra™ Ligation Module

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NEBNext® Ultra™ II Ligation Module 

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NEBNext® Ultra™ II Q5® Master Mix

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NEBNext® Ultra™ II End Repair/dA-Tailing Module

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NEBNext® Ultra™ DNA Library Prep Kit for Illumina®

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NEBNext® Library Dilution Buffer

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NEBNext® FFPE DNA Repair Mix

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NEBNext® Multiplex Oligos for Illumina® (96 Index Primers)

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NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 4)

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NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (Set 1)

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NEBNext® rRNA Depletion Kit (Human/Mouse/Rat)

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NEBNext® Ultra™ II DNA Library Prep Kit for Illumina®

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NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina®

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NEBNext® Ultra™ RNA Library Prep Kit for Illumina®

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NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina®

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NEBNext® Ultra II Directional RNA Library Prep with Sample Purification Beads

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NEBNext® Ultra II DNA Library Prep with Sample Purification Beads

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NEBNext® Ultra II RNA First Strand Synthesis Module

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NEBNext® Ultra II RNA Library Prep Kit for Illumina®

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NEBNext® Ultra II RNA Library Prep with Sample Purification Beads

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NEBNext® Library Quant Kit for Illumina®

NEBNext® Ultra™ II DNA Library Prep

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NEBNext® Ultra™ II Ligation Module 

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NEBNext® Ultra™ II End Repair/dA-Tailing Module

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NEBNext® Ultra™ II DNA Library Prep Kit for Illumina®

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NEBNext® Ultra II DNA Library Prep with Sample Purification Beads

NEBNext® Ultra™ II RNA Library Prep

DNA for Illumina®

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NEBNext® ChIP-Seq Library Prep Master Mix Set for Illumina®

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NEBNext® ChIP-Seq Library Prep Reagent Set for Illumina®

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NEBNext® dA-Tailing Module

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NEBNext® DNA Library Prep Master Mix Set for Illumina®

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NEBNext® DNA Library Prep Reagent Set for Illumina®

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NEBNext® End Repair Module

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NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 2)

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NEBNext® Multiplex Oligos for Illumina® (Dual Index Primers Set 1)

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NEBNext® Quick Ligation Module

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NEBNext® Ultra™ End Repair/dA-Tailing Module

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NEBNext® Ultra™ Ligation Module

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NEBNext® Ultra™ DNA Library Prep Kit for Illumina®

RNA for Illumina®

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NEBNext® Magnesium RNA Fragmentation Module

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NEBNext® mRNA Library Prep Master Mix Set for Illumina®

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NEBNext® mRNA Library Prep Reagent Set for Illumina®

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NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 1)

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NEBNext® Multiplex Oligos for Illumina® (Methylated Adaptor, Index Primers Set 1)

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NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (Set 2)

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NEBNext® Poly(A) mRNA Magnetic Isolation Module

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NEBNext® RNA First Strand Synthesis Module

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NEBNext® Singleplex Oligos for Illumina®

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NEBNext® Small RNA Library Prep Set for Illumina® (Multiplex Compatible)

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NEBNext® Ultra™ II Directional RNA Second Strand Synthesis Module

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NEBNext® Ultra II Non-Directional RNA Second Strand Synthesis Module

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NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (Set 1)

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NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina®

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NEBNext® Ultra™ RNA Library Prep Kit for Illumina®

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NEBNext® Ultra II RNA First Strand Synthesis Module

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NEBNext® Ultra II RNA Library Prep Kit for Illumina®

Ion Torrent™ DNA Library Preparation

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NEBNext® Fast DNA Fragmentation & Library Prep Set for Ion Torrent™

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NEBNext® Fast DNA Library Prep Set for Ion Torrent™

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NEBNext® Q5® Hot Start HiFi PCR Master Mix

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NEBNext® FFPE DNA Repair Mix

DNA for Ion Torrent™

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NEBNext® Fast DNA Fragmentation & Library Prep Set for Ion Torrent™

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NEBNext® Fast DNA Library Prep Set for Ion Torrent™

454™ Library Preparation

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NEBNext® DNA Library Prep Master Mix Set for 454™

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NEBNext® End Repair Module

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NEBNext® Magnesium RNA Fragmentation Module

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NEBNext® Poly(A) mRNA Magnetic Isolation Module

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NEBNext® Quick Ligation Module

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NEBNext® Ultra II Non-Directional RNA Second Strand Synthesis Module

DNA for 454™

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NEBNext® DNA Library Prep Master Mix Set for 454™

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NEBNext® End Repair Module

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NEBNext® Quick Ligation Module

RNA for 454™

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NEBNext® Magnesium RNA Fragmentation Module

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NEBNext® Poly(A) mRNA Magnetic Isolation Module

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NEBNext® Ultra II Non-Directional RNA Second Strand Synthesis Module

DNA Library Preparation

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NEBNext® High-Fidelity 2X PCR Master Mix

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NEBNext Direct® BRCA1/BRCA2 Panel

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NEBNext Direct® Cancer HotSpot Panel

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NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 3)

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NEBNext® dA-Tailing Reaction Buffer

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NEBNext® End Repair Reaction Buffer

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NEBNext® Q5® Hot Start HiFi PCR Master Mix

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NEBNext® Quick Ligation Reaction Buffer

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NEBNext® Ultra™ II Ligation Module 

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NEBNext® Ultra™ II Q5® Master Mix

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NEBNext® Ultra™ II End Repair/dA-Tailing Module

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NEBNext® Library Dilution Buffer

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NEBNext® FFPE DNA Repair Mix

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NEBNext® Multiplex Oligos for Illumina® (96 Index Primers)

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NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 4)

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NEBNext® Ultra™ II DNA Library Prep Kit for Illumina®

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NEBNext® Ultra II DNA Library Prep with Sample Purification Beads

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NEBNext® Library Quant Kit for Illumina®

RNA Library Preparation

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5´ DNA Adenylation Kit

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NEBNext® Multiplex Small RNA Library Prep Kit for Illumina® (Index Primers 1-48)

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NEBNext® Q5® Hot Start HiFi PCR Master Mix

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NEBNext® Second Strand Synthesis (dNTP-free) Reaction Buffer

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NEBNext® Library Dilution Buffer

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NEBNext® rRNA Depletion Kit (Human/Mouse/Rat)

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NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina®

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NEBNext® Ultra II Directional RNA Library Prep with Sample Purification Beads

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NEBNext® Ultra II RNA First Strand Synthesis Module

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NEBNext® Ultra II RNA Library Prep Kit for Illumina®

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NEBNext® Ultra II RNA Library Prep with Sample Purification Beads

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NEBNext® Library Quant Kit for Illumina®

DNA Fragmentation

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NEBNext® Fast DNA Fragmentation & Library Prep Set for Ion Torrent™

NEBNext® dsDNA Fragmentase®

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NEBNext® dsDNA Fragmentase® Reaction Buffer

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NEBNext® dsDNA Fragmentase®

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NEBNext® dsDNA Fragmentase® Reaction Buffer v2

NEBNext® Automation

FFPE DNA Repair

NEBNext FFPE DNA Repair Mix

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