Next Generation Sequencing

SOLiD™ Library Preparation

Library preparation of fragmented DNA, ChIP DNA, and cDNA prepared from mRNA, for use with the SOLiD™ 4 sequencing platform, involves repair of 3’ and 5’ ends before ligation with an adaptor. Small RNA libraries are constructed by a different workflow, in which adaptors are ligated directly to the small RNA molecules, followed by reverse transcription and PCR amplification. Libraries prepared from DNA and mRNA are size selected after adaptor ligation and amplified via polymerase chain reaction (PCR). For more information, choose the workflow tabs below.

Libraries prepared in this manner are suitable for templated bead preparation, the next step in the SOLiD sequencing workflow.

NEBNext® reagents are available for each step in the library preparation workflow for the SOLiD platform. For DNA, ChIP, mRNA and small RNA library preparation, reagents from NEB are available in a master mix format. Each of these reagents has been functionally validated by preparation of a genomic DNA, ChIP-Seq DNA, mRNA or small RNA library followed by SOLiD sequencing.

SOLiD™ is a trademark owned by Life Technologies, Inc.
NEBNext® is a registered trademark of New England Biolabs, Inc.

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SOLiD™ Library Preparation includes these areas of focus:

DNA for SOLiD™
RNA for SOLiD™

FAQs for SOLiD™ Library Preparation

Protocols for SOLiD™ Library Preparation

Legal and Disclaimers

This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

For more information about commercial rights, please contact NEB's Global Business Development team at gbd@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

DNA Library Construction Workflow for SOLiD

mRNA Library Construction Workflow for SOLiD

Small RNA Library Construction Workflow for SOLiD