Protein Expression

Protein Expression and Purification

New England Biolabs is committed to providing advanced tools for protein expression and purification. As a leading supplier for reagents in the biotechnology field, NEB understands the importance of convenient and easy-to-use systems for high level expression and purification of proteins. We invite you to review our growing range of expression systems resulting from our 30 years of experience in cloning, over-expression and purification.

Protein Expression & Purification

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Nuclease-free Water

Protein Expression Approaches

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E. coli Ribosome

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Amylose Magnetic Beads

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Amylose Resin

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Amylose Resin High Flow

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Anti-CBD Monoclonal Antibody

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Anti-GLuc Antibody

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Anti-MBP Monoclonal Antibody

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Anti-MBP Monoclonal Antibody (HRP conjugated)

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BL21 Competent E. coli

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BL21(DE3) Competent E. coli

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Enterokinase, light chain

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Furin

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IMPACT™ Kit

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K. lactis GG799 Competent Cells

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K. lactis Protein Expression Kit

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Lemo21(DE3) Competent E. coli

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NEB® 10-beta Electrocompetent E. coli

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NEB® 5-alpha Electrocompetent E. coli

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NEB® Express Iq Competent E. coli (High Efficiency)

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NEB® Express Competent E. coli (High Efficiency)

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NEB® Turbo Electrocompetent E. coli

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NiCo21(DE3) Competent E. coli

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pCLuc-Basic 2 Vector

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pCMV-CLuc 2 Control Plasmid

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pKLAC2 Vector

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pMAL™ Protein Fusion and Purification System

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pMAL™-c5X Vector

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pMAL™-p5X Vector

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pSNAP-tag® (T7)-2 Vector

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pSV40-CLuc Control Plasmid

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pTXB1 Vector

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pTYB21 Vector

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PURExpress® Δ (aa, tRNA) Kit

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PURExpress® Δ RF123 Kit

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PURExpress® Δ Ribosome Kit

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PURExpress® In Vitro Protein Synthesis Kit

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PURExpress® Disulfide Bond Enhancer

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RNase Inhibitor, Murine

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SHuffle® Express Competent E. coli

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SHuffle® T7 Competent E. coli

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SHuffle® T7 Express lysY Competent E. coli

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SHuffle® T7 Express Competent E. coli

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T7 Express lysY/Iq Competent E. coli (High Efficiency)

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T7 Express Competent E. coli (High Efficiency)

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T7 Express Crystal Competent E. coli (High Efficiency)

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T7 Express Iq Competent E. coli (High Efficiency)

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T7 Express lysY Competent E. coli (High Efficiency)

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Yeast Carbon Base Medium Powder

Coupled Protein Expression & Purification

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Amylose Resin

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Amylose Resin High Flow

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BL21 Competent E. coli

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BL21(DE3) Competent E. coli

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Chitin Resin

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IMPACT™ Kit

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K. lactis Protein Expression Kit

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Lemo21(DE3) Competent E. coli

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NEB® Express Iq Competent E. coli (High Efficiency)

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NEB® Express Competent E. coli (High Efficiency)

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NiCo21(DE3) Competent E. coli

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pMAL™ Protein Fusion and Purification System

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T7 Express lysY/Iq Competent E. coli (High Efficiency)

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T7 Express Competent E. coli (High Efficiency)

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T7 Express Iq Competent E. coli (High Efficiency)

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T7 Express lysY Competent E. coli (High Efficiency)

Expression of Difficult Proteins

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Lemo21(DE3) Competent E. coli

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PURExpress® In Vitro Protein Synthesis Kit

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PURExpress® Disulfide Bond Enhancer

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SHuffle® Express Competent E. coli

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SHuffle® T7 Competent E. coli

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SHuffle® T7 Express lysY Competent E. coli

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SHuffle® T7 Express Competent E. coli

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T7 Express lysY/Iq Competent E. coli (High Efficiency)

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T7 Express Iq Competent E. coli (High Efficiency)

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T7 Express lysY Competent E. coli (High Efficiency)

Affinity Purification

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12-Tube Magnetic Separation Rack

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50 ml Magnetic Separation Rack

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6-Tube Magnetic Separation Rack

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96-Well Microtiter Plate Magnetic Separation Rack

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Amylose Magnetic Beads

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Anti-MBP Magnetic Beads

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Chitin Magnetic Beads

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Chitin Resin

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Goat Anti-Mouse IgG Magnetic Beads

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Goat Anti-Rabbit IgG Magnetic Beads

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Goat Anti-Rat IgG Magnetic Beads

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Hydrophilic Streptavidin Magnetic Beads

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pMAL™ Protein Fusion and Purification System

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Protein A Magnetic Beads

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Protein G Magnetic Beads

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SNAP-Capture Magnetic Beads

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Streptavidin Magnetic Beads

Protein Expression Approaches

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E. coli Ribosome

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Anti-GLuc Antibody

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BL21 Competent E. coli

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Enterokinase, light chain

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Furin

E.coli Protein Expression

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Amylose Magnetic Beads

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Amylose Resin

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Amylose Resin High Flow

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Anti-CBD Monoclonal Antibody

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Anti-MBP Monoclonal Antibody

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Anti-MBP Monoclonal Antibody (HRP conjugated)

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BL21 Competent E. coli

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BL21(DE3) Competent E. coli

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Enterokinase, light chain

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Furin

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IMPACT™ Kit

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Lemo21(DE3) Competent E. coli

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NEB® 10-beta Electrocompetent E. coli

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NEB® 5-alpha Electrocompetent E. coli

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NEB® Express Iq Competent E. coli (High Efficiency)

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NEB® Express Competent E. coli (High Efficiency)

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NEB® Turbo Electrocompetent E. coli

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NiCo21(DE3) Competent E. coli

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pMAL™ Protein Fusion and Purification System

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pMAL™-c5X Vector

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pMAL™-p5X Vector

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pSNAP-tag® (T7)-2 Vector

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pTXB1 Vector

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pTYB21 Vector

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SHuffle® Express Competent E. coli

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SHuffle® T7 Competent E. coli

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SHuffle® T7 Express lysY Competent E. coli

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SHuffle® T7 Express Competent E. coli

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T7 Express lysY/Iq Competent E. coli (High Efficiency)

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T7 Express Competent E. coli (High Efficiency)

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T7 Express Crystal Competent E. coli (High Efficiency)

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T7 Express Iq Competent E. coli (High Efficiency)

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T7 Express lysY Competent E. coli (High Efficiency)

Yeast Protein Expression

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K. lactis GG799 Competent Cells

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K. lactis Protein Expression Kit

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pCLuc-Basic 2 Vector

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pCMV-CLuc 2 Control Plasmid

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pKLAC2 Vector

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pSV40-CLuc Control Plasmid

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pTYB21 Vector

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Yeast Carbon Base Medium Powder

Cell-Free Protein Expression

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E. coli Ribosome

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pTYB21 Vector

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PURExpress® Δ (aa, tRNA) Kit

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PURExpress® Δ RF123 Kit

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PURExpress® Δ Ribosome Kit

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PURExpress® In Vitro Protein Synthesis Kit

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PURExpress® Disulfide Bond Enhancer

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RNase Inhibitor, Murine

Coupled Protein Expression & Purification

Maltose Binding Protein Expression

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Amylose Resin

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Amylose Resin High Flow

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BL21 Competent E. coli

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NEB® Express Iq Competent E. coli (High Efficiency)

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NEB® Express Competent E. coli (High Efficiency)

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pMAL™ Protein Fusion and Purification System

IMPACT™ Protein Expression

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BL21(DE3) Competent E. coli

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IMPACT™ Kit

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Lemo21(DE3) Competent E. coli

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NiCo21(DE3) Competent E. coli

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T7 Express lysY/Iq Competent E. coli (High Efficiency)

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T7 Express Competent E. coli (High Efficiency)

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T7 Express Iq Competent E. coli (High Efficiency)

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T7 Express lysY Competent E. coli (High Efficiency)

His-tagged Protein Expression

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NiCo21(DE3) Competent E. coli

Yeast Chitin Binding Domain Tag

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Chitin Resin

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K. lactis Protein Expression Kit

Expression of Difficult Proteins

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SHuffle® T7 Competent E. coli

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SHuffle® T7 Express lysY Competent E. coli

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SHuffle® T7 Express Competent E. coli

Toxic Protein Expression

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Lemo21(DE3) Competent E. coli

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PURExpress® In Vitro Protein Synthesis Kit

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T7 Express lysY/Iq Competent E. coli (High Efficiency)

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T7 Express Iq Competent E. coli (High Efficiency)

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T7 Express lysY Competent E. coli (High Efficiency)

Disulfide-Bonded Protein Expression

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PURExpress® In Vitro Protein Synthesis Kit

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PURExpress® Disulfide Bond Enhancer

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SHuffle® Express Competent E. coli

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SHuffle® T7 Competent E. coli

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SHuffle® T7 Express lysY Competent E. coli

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SHuffle® T7 Express Competent E. coli

Membrane Protein Expression

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Lemo21(DE3) Competent E. coli

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T7 Express lysY/Iq Competent E. coli (High Efficiency)

Affinity Purification

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12-Tube Magnetic Separation Rack

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50 ml Magnetic Separation Rack

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6-Tube Magnetic Separation Rack

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96-Well Microtiter Plate Magnetic Separation Rack

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Amylose Magnetic Beads

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Anti-MBP Magnetic Beads

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Chitin Magnetic Beads

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Chitin Resin

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Goat Anti-Mouse IgG Magnetic Beads

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Goat Anti-Rabbit IgG Magnetic Beads

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Goat Anti-Rat IgG Magnetic Beads

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Hydrophilic Streptavidin Magnetic Beads

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pMAL™ Protein Fusion and Purification System

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Protein A Magnetic Beads

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Protein G Magnetic Beads

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SNAP-Capture Magnetic Beads

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Streptavidin Magnetic Beads

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