Home FAQs Why is the product a smear when visualized on an agarose gel?

FAQ: Why is the product a smear when visualized on an agarose gel?

When PCR conditions are not optimal, a smear or high level of background is often observed. Try one or more of the following suggestions:
  • use lower amount of enzyme
  • use 65°C for extension
  • raise annealing temperature
  • try 2-step cycling protocols