FAQ: What is the Proteinase K activity in commonly used buffers?

Buffer: 30 mM Tris HCl, pH 8.0
Application: Reference
Relative activity (approx.): 100%

Buffer: 50mM Tris-HCl, pH 8.0, 1 mM CaCl2, 3 mM DTT, 2.0 M Urea
Application: Denaturation of proteins
Relative activity (approx.): 70%

Buffer: 100 mM Tris-HCl, pH 8.0, 100 mM EDTA, 250mM NaCl, 1% Sarkosyl
Application: Plant tissue DNA isolation
Relative activity (approx.): 120%

Buffer: 10 mM Tris-HCl, pH 8.0, 1 mM EDTA, 0.5% SDS
Application: Bacterial DNA isolation
Relative activity (approx.): 100%

Buffer: 10 mM Tris HCl, pH 8.0, 50 mM NaCl, 5 mM EDTA, 1 mM DTT, 0.5% SDS 50 mM Tris-HCl, pH 8.0
Application: Denaturation of CIP
Relative activity (approx.): 100%

Buffer: 50 mM EDTA, 5% Tween 20, 0.5% Triton-X 100, 800 mM GuHCl
Relative activity (approx.): 300%