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Product Information
NEB 10-beta electrocompetent E. coli cells are optimized for high efficiency transformation by electroporation. These cells are ideal for DNA library constructions and all cloning purposes.
Highlights
- DH10B™ derivative
- Accomodation of large plasmids including BAC and cosmid constructs
- Efficient transformation of methylated DNA derived from eukaryotic sources and unmethylated DNA derived from PCR, cDNA and other sources
- Activity of nonspecific endonuclease I eliminated for highest quality plasmid preparations (endA1)
- Suitable for blue/white screening by α-complementation of the β-galactosidase gene (Φ80ΔlacZM15)
- Reduced recombination of cloned DNA (recA)
- Resistance to phage T1 (fhuA)
- K-12 Strain
- Free of animal products
Transformation Efficiency
> 2 x 1010 cfu/μg pUC19Genotype
Δ(ara-leu) 7697 araD139 fhuA ΔlacX74 galK16 galE15 e14- Φ80dlacZΔM15 recA1 relA1 endA1 nupG rpsL (StrR) rph spoT1 Δ(mrr-hsdRMS-mcrBC)
- This product is related to the following categories:
- Cloning Competent Cell Strains Products
- This product can be used in the following applications:
- Transformation
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