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  • NEBNext® Ultra™ II Ligation Module 

    Description







     
    The NEBNext Ultra II Ligation Module has been optimized to efficiently ligate DNA adaptors compatible with Illumina® sequencing to end-repaired, dA-tailed DNA fragments. The module is optimized for use with the NEBNext Ultra II End Repair/dA-Tailing Module (NEB #E7546), and is part of the Ultra II workflow which enables high yield preparation of high quality libraries from 500 picograms to 1 microgram of input DNA.

    The NEBNext Ultra II Ligation Module is Designed for use with the Following:
    • NEBNext Ultra II End Repair/dA-Tailing Module (NEB #E7546)
    • NEBNext Ultra II Q5® Master Mix (NEB #M0544)
    • NEBNext Singleplex or Multiplex Oligos for Illumina (NEB #E7350, #E7335, #E7500, #E7600 or #E7535)

    Functional Validation

    Each set of reagents is functionally validated together through construction and sequencing of a transcriptome library and sequenced on a DNA library on the Illumina sequencing platform.

    Lot Control

    The lots provided are managed separately and qualified by additional functional validation. Individual reagents undergo standard enzyme activity and quality control assays, and also meet stringent criteria in the additional quality controls listed on each individual component page

    Reagents Supplied

    The following reagents are supplied with this product:

    Store at (°C)Concentration
    NEBNext Ultra II Ligation Master Mix-20
    NEBNext Ligation Enhancer-20

    Properties and Usage

    Materials Required but not Supplied

    NEBNext Ultra II End Repair/ dA-Tailing Module (NEB #E7546)
    NEBNext Ultra II Q5® Master Mix (NEB #M0544)
    NEBNext Singleplex or Multiplex Oligos for Illumina® (NEB #E7350, #E7335, #E7500, #E7710, #E7730, #E6609, #E7600 or #E7535)

    Storage Temperature

    -20°C

    FAQs

    1. What is the difference between the three NEBNext Ligation Modules?
    2. How do I obtain the NEBNext Adaptor and USER enzyme for use with the NEBNext Ultra II Ligation Module?

    Protocols

    1. Protocol for use with NEBNext® Ultra™ II Ligation Module (E7595)

    Manuals

    The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name these document files: manual[Catalog Number].

    Safety Data Sheet

    The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.