The WarmStart® feature of Bst 2.0 DNA Polymerase ( NEB #M0538) is unique among isothermal polymerases. Like “Hot Start” PCR polymerases, this feature prevents activity at temperatures below the optimal reaction temperature. This enables room temperature reaction set up and minimizes undesirable reaction products, thereby increasing reaction efficiencies.
WarmStart Feature Enables Room Temperature Setup
For Bst DNA Polymerase and Bst 2.0, incubation of the sample at room temperature before beginning the SDA assay results in variable performance. For Bst 2.0 WarmStart, extended pre-assay incubation at room temperature does not affect performance. The different scales highlight the increased reaction speed with Bst 2.0 and Bst 2.0 WarmStart DNA Polymerases over wild-type Bst DNA Polymerase.
In contrast to chemical modifications or antibodies commonly used with hot start PCR polymerases, NEB’s
Bst 2.0 WarmStart DNA Polymerase utilizes aptamer technology. Aptamers are extensively modified, unique oligonucleotides which bind to the polymerase through non-covalent interactions, inhibiting activity at non-permissive temperatures (< 50°C). Additionally, no separate activation step is required for Bst 2.0 WarmStart DNA Polymerase.
While standard formulations of Bst DNA polymerase and homologs add non-templated nucleotides to substrate during room temperature reaction setup, this undesirable activity is eliminated in this WarmStart version.
WarmStart® is a registered trademark of New England Biolabs, Inc.