Polymerases and Amplification Technologies

PCR, qPCR & Amplification Technologies

NEB offers a wide range of DNA polymerases, and through our commitment to research, ensures the development of innovative, high quality tools for DNA amplification, qPCR and PCR. Our amplification technologies product portfolio includes a broad variety of both DNA and RNA polymerases, nucleotide mixes, kits, master mixes and more, featuring:

PCR, qPCR & Amplification Technologies

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Luna® Universal One-Step RT-qPCR Kit

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Luna® Universal Probe One-Step RT-qPCR Kit

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Luna® Universal Probe qPCR Master Mix

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Luna® Universal qPCR Master Mix

Q5® High-Fidelity DNA Polymerases

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NEBNext® High-Fidelity 2X PCR Master Mix

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NEBNext® Q5® Hot Start HiFi PCR Master Mix

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NEBNext® Ultra™ II Q5® Master Mix

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Q5® High-Fidelity 2X Master Mix

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Q5® High-Fidelity DNA Polymerase

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Q5® High-Fidelity PCR Kit

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Q5® Hot Start High-Fidelity DNA Polymerase

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Q5® Hot Start High-Fidelity 2X Master Mix

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Q5® Reaction Buffer Pack

OneTaq® DNA Polymerases

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OneTaq® Quick-Load® 2X Master Mix with GC Buffer

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OneTaq® 2X Master Mix with Standard Buffer

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OneTaq® DNA Polymerase

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OneTaq® Hot Start 2X Master Mix with GC Buffer

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OneTaq® Hot Start 2X Master Mix with Standard Buffer

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OneTaq® Hot Start DNA Polymerase

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OneTaq® Hot Start Quick-Load® 2X Master Mix with Standard Buffer

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OneTaq® Quick-Load® 2X Master Mix with Standard Buffer

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OneTaq® 2X Master Mix with GC Buffer

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OneTaq® Hot Start Quick-Load® 2X Master Mix with GC Buffer

Taq DNA Polymerases

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Taq 2X Master Mix

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Taq 5X Master Mix

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Taq DNA Polymerase with Standard Taq Buffer

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Taq DNA Polymerase with ThermoPol® Buffer

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Taq PCR Kit

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Taq DNA Polymerase with Standard Taq (Mg-free) Buffer

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EpiMark® Hot Start Taq DNA Polymerase

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Hot Start Taq DNA Polymerase

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Hot Start Taq 2X Master Mix

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Multiplex PCR 5X Master Mix

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OneTaq Hot Start 2X Master Mix with Standard Buffer

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OneTaq® Quick-Load® 2X Master Mix with GC Buffer

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OneTaq® 2X Master Mix with Standard Buffer

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OneTaq® DNA Polymerase

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OneTaq® Hot Start 2X Master Mix with GC Buffer

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OneTaq® Hot Start 2X Master Mix with Standard Buffer

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OneTaq® Hot Start DNA Polymerase

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OneTaq® Hot Start Quick-Load® 2X Master Mix with Standard Buffer

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OneTaq® Quick-Load® 2X Master Mix with Standard Buffer

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OneTaq® 2X Master Mix with GC Buffer

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OneTaq® Hot Start Quick-Load® 2X Master Mix with GC Buffer

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Quick-Load® Taq 2X Master Mix

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Standard Taq (Mg-free) Reaction Buffer Pack

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Standard Taq Reaction Buffer Pack

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ThermoPol® II (Mg-free) Reaction Buffer Pack

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ThermoPol® Reaction Buffer Pack

LongAmp™ DNA Polymerases

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LongAmp® Taq DNA Polymerase

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LongAmp® Taq PCR Kit

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LongAmp® Taq 2X Master Mix

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LongAmp® Hot Start Taq 2X Master Mix

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LongAmp® Hot Start Taq DNA Polymerase

Luna® qPCR & RT-qPCR Products

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Luna® Universal One-Step RT-qPCR Kit

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Luna® Universal Probe One-Step RT-qPCR Kit

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Luna® Universal Probe qPCR Master Mix

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Luna® Universal qPCR Master Mix

Other PCR Polymerases

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Deep Vent® (exo-) DNA Polymerase

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Deep Vent® DNA Polymerase

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Hemo KlenTaq®

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Vent® (exo-) DNA Polymerase

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Vent® DNA Polymerase

Phusion® High-Fidelity DNA Polymerases

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Phusion® GC Buffer Pack

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Phusion® HF Buffer Pack

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Phusion® High-Fidelity DNA Polymerase

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Phusion® High-Fidelity PCR Kit

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Phusion® High-Fidelity PCR Master Mix with GC Buffer

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Phusion® High-Fidelity PCR Master Mix with HF Buffer

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Phusion® Hot Start Flex 2X Master Mix

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Phusion® Hot Start Flex DNA Polymerase

Master Mixes

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Taq 2X Master Mix

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Taq 5X Master Mix

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Hot Start Taq 2X Master Mix

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LongAmp® Taq 2X Master Mix

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LongAmp® Hot Start Taq 2X Master Mix

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Multiplex PCR 5X Master Mix

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NEBNext® Q5® Hot Start HiFi PCR Master Mix

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OneTaq® Quick-Load® 2X Master Mix with GC Buffer

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OneTaq® 2X Master Mix with Standard Buffer

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OneTaq® Hot Start 2X Master Mix with GC Buffer

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OneTaq® Hot Start 2X Master Mix with Standard Buffer

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OneTaq® Hot Start Quick-Load® 2X Master Mix with Standard Buffer

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OneTaq® Quick-Load® 2X Master Mix with Standard Buffer

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OneTaq® 2X Master Mix with GC Buffer

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OneTaq® Hot Start Quick-Load® 2X Master Mix with GC Buffer

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Phusion® High-Fidelity PCR Master Mix with GC Buffer

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Phusion® High-Fidelity PCR Master Mix with HF Buffer

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Phusion® Hot Start Flex 2X Master Mix

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Q5® High-Fidelity 2X Master Mix

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Q5® Hot Start High-Fidelity 2X Master Mix

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Quick-Load® Taq 2X Master Mix

DNA Manipulation

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Bsu DNA Polymerase, Large Fragment

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Sulfolobus DNA Polymerase IV

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DNA Polymerase I (E. coli)

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DNA Polymerase I, Large (Klenow) Fragment

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Klenow Fragment (3'→5' exo-)

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PreCR® Repair Mix

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Quick Blunting™ Kit

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T4 DNA Polymerase

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T7 DNA Polymerase (unmodified)

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Terminal Transferase

Isothermal Amplification & Strand Displacement

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Bst 2.0 WarmStart® DNA Polymerase

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Bst 3.0 DNA Polymerase

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Bst DNA Polymerase, Full Length

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Bst DNA Polymerase, Large Fragment

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IsoAmp® II Universal tHDA Kit

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Isothermal Amplification Buffer II Pack

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Isothermal Amplification Buffer Pack

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Klenow Fragment (3'→5' exo-)

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phi29 DNA Polymerase

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WarmStart® Colorimetric LAMP 2X Master Mix (DNA & RNA)

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WarmStart® LAMP Kit (DNA & RNA)

Nucleotide Solutions

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3´-Biotin-GTP

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3´-Desthiobiotin-GTP

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5-methyl-dCTP

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7-deaza-dGTP

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Acyclonucleotide Set

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Adenosine 5'-Triphosphate (ATP)

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dATP Solution

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Deoxynucleotide (dNTP) Solution Mix

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Deoxynucleotide (dNTP) Solution Set

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Ribonucleotide Solution Set

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Ribonucleotide Solution Mix

cDNA Synthesis & Reverse Transcriptases

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E. coli Poly(A) Polymerase

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AMV Reverse Transcriptase

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DNA Polymerase I (E. coli)

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M13KO7 Helper Phage

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M-MuLV Reverse Transcriptase

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NEBNext® Ultra II Non-Directional RNA Second Strand Synthesis Module

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Oligo d(T)18 mRNA Primer

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Oligo d(T)23 VN

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OneTaq® One-Step RT-PCR Kit

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OneTaq® RT-PCR Kit

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Poly(U) Polymerase

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ProtoScript® II First Strand cDNA Synthesis Kit

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ProtoScript® II Reverse Transcriptase

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ProtoScript® First Strand cDNA Synthesis Kit

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Random Primer 9

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RNase H

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RNase Inhibitor, Murine

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SP6 RNA Polymerase

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T3 RNA Polymerase

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T4 RNA Ligase 2, truncated KQ

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WarmStart® RTx Reverse Transcriptase

RT-PCR

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AMV Reverse Transcriptase

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M-MuLV Reverse Transcriptase

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Oligo d(T)18 mRNA Primer

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Oligo d(T)23 VN

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OneTaq® One-Step RT-PCR Kit

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OneTaq® RT-PCR Kit

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ProtoScript® II First Strand cDNA Synthesis Kit

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ProtoScript® II Reverse Transcriptase

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ProtoScript® First Strand cDNA Synthesis Kit

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Random Primer 9

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T4 RNA Ligase 2, truncated KQ

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WarmStart® RTx Reverse Transcriptase

Reaction Buffers

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β-Nicotinamide adenine dinucleotide (NAD+)

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Isothermal Amplification Buffer II Pack

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Isothermal Amplification Buffer Pack

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Magnesium Chloride (MgCl2) Solution

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Magnesium Sulfate (MgSO4) Solution

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Phusion® GC Buffer Pack

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Phusion® HF Buffer Pack

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Q5® Reaction Buffer Pack

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Standard Taq (Mg-free) Reaction Buffer Pack

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Standard Taq Reaction Buffer Pack

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ThermoPol® II (Mg-free) Reaction Buffer Pack

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ThermoPol® Reaction Buffer Pack

Q5® High-Fidelity DNA Polymerases

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NEBNext® High-Fidelity 2X PCR Master Mix

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NEBNext® Q5® Hot Start HiFi PCR Master Mix

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NEBNext® Ultra™ II Q5® Master Mix

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Q5® High-Fidelity 2X Master Mix

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Q5® High-Fidelity DNA Polymerase

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Q5® High-Fidelity PCR Kit

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Q5® Hot Start High-Fidelity DNA Polymerase

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Q5® Hot Start High-Fidelity 2X Master Mix

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Q5® Reaction Buffer Pack

OneTaq® DNA Polymerases

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OneTaq® Quick-Load® 2X Master Mix with GC Buffer

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OneTaq® 2X Master Mix with Standard Buffer

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OneTaq® DNA Polymerase

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OneTaq® Hot Start 2X Master Mix with GC Buffer

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OneTaq® Hot Start 2X Master Mix with Standard Buffer

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OneTaq® Hot Start DNA Polymerase

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OneTaq® Hot Start Quick-Load® 2X Master Mix with Standard Buffer

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OneTaq® Quick-Load® 2X Master Mix with Standard Buffer

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OneTaq® 2X Master Mix with GC Buffer

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OneTaq® Hot Start Quick-Load® 2X Master Mix with GC Buffer

Taq DNA Polymerases

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Taq 2X Master Mix

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Taq 5X Master Mix

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Taq DNA Polymerase with Standard Taq Buffer

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Taq DNA Polymerase with ThermoPol® Buffer

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Taq PCR Kit

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Taq DNA Polymerase with Standard Taq (Mg-free) Buffer

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EpiMark® Hot Start Taq DNA Polymerase

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Hot Start Taq DNA Polymerase

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Hot Start Taq 2X Master Mix

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Multiplex PCR 5X Master Mix

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OneTaq Hot Start 2X Master Mix with Standard Buffer

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OneTaq® Quick-Load® 2X Master Mix with GC Buffer

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OneTaq® 2X Master Mix with Standard Buffer

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OneTaq® DNA Polymerase

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OneTaq® Hot Start 2X Master Mix with GC Buffer

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OneTaq® Hot Start 2X Master Mix with Standard Buffer

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OneTaq® Hot Start DNA Polymerase

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OneTaq® Hot Start Quick-Load® 2X Master Mix with Standard Buffer

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OneTaq® Quick-Load® 2X Master Mix with Standard Buffer

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OneTaq® 2X Master Mix with GC Buffer

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OneTaq® Hot Start Quick-Load® 2X Master Mix with GC Buffer

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Quick-Load® Taq 2X Master Mix

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Standard Taq (Mg-free) Reaction Buffer Pack

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Standard Taq Reaction Buffer Pack

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ThermoPol® II (Mg-free) Reaction Buffer Pack

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ThermoPol® Reaction Buffer Pack

LongAmp™ DNA Polymerases

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LongAmp® Taq DNA Polymerase

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LongAmp® Taq PCR Kit

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LongAmp® Taq 2X Master Mix

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LongAmp® Hot Start Taq 2X Master Mix

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LongAmp® Hot Start Taq DNA Polymerase

Luna® qPCR & RT-qPCR Products

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Luna® Universal One-Step RT-qPCR Kit

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Luna® Universal Probe One-Step RT-qPCR Kit

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Luna® Universal Probe qPCR Master Mix

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Luna® Universal qPCR Master Mix

Other PCR Polymerases

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Deep Vent® (exo-) DNA Polymerase

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Deep Vent® DNA Polymerase

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Hemo KlenTaq®

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Vent® (exo-) DNA Polymerase

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Vent® DNA Polymerase

Phusion® High-Fidelity DNA Polymerases

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Phusion® GC Buffer Pack

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Phusion® HF Buffer Pack

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Phusion® High-Fidelity DNA Polymerase

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Phusion® High-Fidelity PCR Kit

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Phusion® High-Fidelity PCR Master Mix with GC Buffer

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Phusion® High-Fidelity PCR Master Mix with HF Buffer

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Phusion® Hot Start Flex 2X Master Mix

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Phusion® Hot Start Flex DNA Polymerase

Master Mixes

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Taq 2X Master Mix

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Taq 5X Master Mix

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Hot Start Taq 2X Master Mix

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LongAmp® Taq 2X Master Mix

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LongAmp® Hot Start Taq 2X Master Mix

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Multiplex PCR 5X Master Mix

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NEBNext® Q5® Hot Start HiFi PCR Master Mix

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OneTaq® Quick-Load® 2X Master Mix with GC Buffer

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OneTaq® 2X Master Mix with Standard Buffer

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OneTaq® Hot Start 2X Master Mix with GC Buffer

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OneTaq® Hot Start 2X Master Mix with Standard Buffer

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OneTaq® Hot Start Quick-Load® 2X Master Mix with Standard Buffer

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OneTaq® Quick-Load® 2X Master Mix with Standard Buffer

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OneTaq® 2X Master Mix with GC Buffer

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OneTaq® Hot Start Quick-Load® 2X Master Mix with GC Buffer

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Phusion® High-Fidelity PCR Master Mix with GC Buffer

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Phusion® High-Fidelity PCR Master Mix with HF Buffer

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Phusion® Hot Start Flex 2X Master Mix

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Q5® High-Fidelity 2X Master Mix

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Q5® Hot Start High-Fidelity 2X Master Mix

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Quick-Load® Taq 2X Master Mix

DNA Manipulation

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Bsu DNA Polymerase, Large Fragment

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Sulfolobus DNA Polymerase IV

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DNA Polymerase I (E. coli)

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DNA Polymerase I, Large (Klenow) Fragment

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Klenow Fragment (3'→5' exo-)

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PreCR® Repair Mix

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Quick Blunting™ Kit

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T4 DNA Polymerase

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T7 DNA Polymerase (unmodified)

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Terminal Transferase

Isothermal Amplification & Strand Displacement

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Bst 2.0 WarmStart® DNA Polymerase

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Bst 3.0 DNA Polymerase

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Bst DNA Polymerase, Full Length

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Bst DNA Polymerase, Large Fragment

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IsoAmp® II Universal tHDA Kit

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Isothermal Amplification Buffer II Pack

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Isothermal Amplification Buffer Pack

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Klenow Fragment (3'→5' exo-)

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phi29 DNA Polymerase

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WarmStart® Colorimetric LAMP 2X Master Mix (DNA & RNA)

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WarmStart® LAMP Kit (DNA & RNA)

Nucleotide Solutions

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3´-Biotin-GTP

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3´-Desthiobiotin-GTP

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5-methyl-dCTP

Close

7-deaza-dGTP

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Acyclonucleotide Set

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Adenosine 5'-Triphosphate (ATP)

Close

dATP Solution

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Deoxynucleotide (dNTP) Solution Mix

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Deoxynucleotide (dNTP) Solution Set

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Ribonucleotide Solution Set

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Ribonucleotide Solution Mix

cDNA Synthesis & Reverse Transcriptases

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E. coli Poly(A) Polymerase

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AMV Reverse Transcriptase

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DNA Polymerase I (E. coli)

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M13KO7 Helper Phage

Close

M-MuLV Reverse Transcriptase

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NEBNext® Ultra II Non-Directional RNA Second Strand Synthesis Module

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Oligo d(T)18 mRNA Primer

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Oligo d(T)23 VN

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OneTaq® One-Step RT-PCR Kit

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OneTaq® RT-PCR Kit

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Poly(U) Polymerase

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ProtoScript® II First Strand cDNA Synthesis Kit

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ProtoScript® II Reverse Transcriptase

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ProtoScript® First Strand cDNA Synthesis Kit

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Random Primer 9

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RNase H

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RNase Inhibitor, Murine

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SP6 RNA Polymerase

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T3 RNA Polymerase

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T4 RNA Ligase 2, truncated KQ

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WarmStart® RTx Reverse Transcriptase

RT-PCR

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AMV Reverse Transcriptase

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M-MuLV Reverse Transcriptase

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Oligo d(T)18 mRNA Primer

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Oligo d(T)23 VN

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OneTaq® One-Step RT-PCR Kit

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OneTaq® RT-PCR Kit

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ProtoScript® II First Strand cDNA Synthesis Kit

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ProtoScript® II Reverse Transcriptase

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ProtoScript® First Strand cDNA Synthesis Kit

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Random Primer 9

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T4 RNA Ligase 2, truncated KQ

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WarmStart® RTx Reverse Transcriptase

Reaction Buffers

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β-Nicotinamide adenine dinucleotide (NAD+)

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Isothermal Amplification Buffer II Pack

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Isothermal Amplification Buffer Pack

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Magnesium Chloride (MgCl2) Solution

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Magnesium Sulfate (MgSO4) Solution

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Phusion® GC Buffer Pack

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Phusion® HF Buffer Pack

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Q5® Reaction Buffer Pack

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Standard Taq (Mg-free) Reaction Buffer Pack

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Standard Taq Reaction Buffer Pack

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ThermoPol® II (Mg-free) Reaction Buffer Pack

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ThermoPol® Reaction Buffer Pack

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