Polymerases and Amplification Technologies

PCR, qPCR & Amplification Technologies

NEB offers a wide range of DNA polymerases, and through our commitment to research, ensures the development of innovative, high quality tools for DNA amplification, qPCR and PCR. Our amplification technologies product portfolio includes a broad variety of both DNA and RNA polymerases, nucleotide mixes, kits, master mixes and more, featuring:

PCR, qPCR & Amplification Technologies

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OrderLuna® Universal One-Step RT-qPCR Kit

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OrderLuna® Universal Probe One-Step RT-qPCR Kit

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OrderLuna® Universal Probe qPCR Master Mix

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OrderLuna® Universal qPCR Master Mix

Q5® High-Fidelity DNA Polymerases

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OrderNEBNext® High-Fidelity 2X PCR Master Mix

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OrderNEBNext® Q5® Hot Start HiFi PCR Master Mix

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OrderNEBNext® Ultra™ II Q5® Master Mix

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OrderQ5® High-Fidelity 2X Master Mix

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OrderQ5® High-Fidelity DNA Polymerase

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OrderQ5® High-Fidelity PCR Kit

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OrderQ5® Hot Start High-Fidelity DNA Polymerase

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OrderQ5® Hot Start High-Fidelity 2X Master Mix

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OrderQ5® Reaction Buffer Pack

OneTaq® DNA Polymerases

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OrderOneTaq® Quick-Load® 2X Master Mix with GC Buffer

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OrderOneTaq® 2X Master Mix with Standard Buffer

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OrderOneTaq® DNA Polymerase

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OrderOneTaq® GC Reaction Buffer Pack

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OrderOneTaq® Hot Start 2X Master Mix with GC Buffer

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OrderOneTaq® Hot Start 2X Master Mix with Standard Buffer

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OrderOneTaq® Hot Start DNA Polymerase

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OrderOneTaq® Hot Start Quick-Load® 2X Master Mix with Standard Buffer

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OrderOneTaq® Quick-Load® 2X Master Mix with Standard Buffer

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OrderOneTaq® 2X Master Mix with GC Buffer

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OrderOneTaq® Hot Start Quick-Load® 2X Master Mix with GC Buffer

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OrderOneTaq® Standard Reaction Buffer Pack

Taq DNA Polymerases

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OrderTaq 2X Master Mix

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OrderTaq 5X Master Mix

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OrderTaq DNA Polymerase with Standard Taq Buffer

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OrderTaq DNA Polymerase with ThermoPol® Buffer

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OrderTaq PCR Kit

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OrderTaq DNA Polymerase with Standard Taq (Mg-free) Buffer

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OrderEpiMark® Hot Start Taq DNA Polymerase

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OrderHot Start Taq DNA Polymerase

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OrderHot Start Taq 2X Master Mix

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OrderMultiplex PCR 5X Master Mix

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OneTaq Hot Start 2X Master Mix with Standard Buffer

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OrderOneTaq® Quick-Load® 2X Master Mix with GC Buffer

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OrderOneTaq® 2X Master Mix with Standard Buffer

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OrderOneTaq® DNA Polymerase

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OrderOneTaq® GC Reaction Buffer Pack

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OrderOneTaq® Hot Start 2X Master Mix with GC Buffer

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OrderOneTaq® Hot Start 2X Master Mix with Standard Buffer

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OrderOneTaq® Hot Start DNA Polymerase

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OrderOneTaq® Hot Start Quick-Load® 2X Master Mix with Standard Buffer

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OrderOneTaq® Quick-Load® 2X Master Mix with Standard Buffer

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OrderOneTaq® 2X Master Mix with GC Buffer

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OrderOneTaq® Hot Start Quick-Load® 2X Master Mix with GC Buffer

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OrderOneTaq® Standard Reaction Buffer Pack

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OrderQuick-Load® Taq 2X Master Mix

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OrderStandard Taq (Mg-free) Reaction Buffer Pack

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OrderStandard Taq Reaction Buffer Pack

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OrderThermoPol® II (Mg-free) Reaction Buffer Pack

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OrderThermoPol® Reaction Buffer Pack

LongAmp™ DNA Polymerases

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OrderLongAmp® Taq DNA Polymerase

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OrderLongAmp® Taq PCR Kit

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OrderLongAmp® Taq Reaction Buffer Pack

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OrderLongAmp® Taq 2X Master Mix

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OrderLongAmp® Hot Start Taq 2X Master Mix

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OrderLongAmp® Hot Start Taq DNA Polymerase

Luna qPCR & RT-qPCR Products

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OrderLuna® Universal One-Step RT-qPCR Kit

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OrderLuna® Universal Probe One-Step RT-qPCR Kit

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OrderLuna® Universal Probe qPCR Master Mix

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OrderLuna® Universal qPCR Master Mix

Other PCR Polymerases

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OrderDeep Vent® (exo-) DNA Polymerase

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OrderDeep Vent® DNA Polymerase

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OrderHemo KlenTaq®

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OrderVent® (exo-) DNA Polymerase

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OrderVent® DNA Polymerase

Phusion® High-Fidelity DNA Polymerases

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OrderPhusion® GC Buffer Pack

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OrderPhusion® HF Buffer Pack

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OrderPhusion® High-Fidelity DNA Polymerase

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OrderPhusion® High-Fidelity PCR Kit

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OrderPhusion® High-Fidelity PCR Master Mix with GC Buffer

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OrderPhusion® High-Fidelity PCR Master Mix with HF Buffer

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OrderPhusion® Hot Start Flex 2X Master Mix

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OrderPhusion® Hot Start Flex DNA Polymerase

Master Mixes

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OrderTaq 2X Master Mix

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OrderTaq 5X Master Mix

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OrderHot Start Taq 2X Master Mix

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OrderLongAmp® Taq 2X Master Mix

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OrderLongAmp® Hot Start Taq 2X Master Mix

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OrderMultiplex PCR 5X Master Mix

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OrderNEBNext® Q5® Hot Start HiFi PCR Master Mix

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OrderOneTaq® Quick-Load® 2X Master Mix with GC Buffer

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OrderOneTaq® 2X Master Mix with Standard Buffer

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OrderOneTaq® Hot Start 2X Master Mix with GC Buffer

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OrderOneTaq® Hot Start 2X Master Mix with Standard Buffer

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OrderOneTaq® Hot Start Quick-Load® 2X Master Mix with Standard Buffer

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OrderOneTaq® Quick-Load® 2X Master Mix with Standard Buffer

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OrderOneTaq® 2X Master Mix with GC Buffer

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OrderOneTaq® Hot Start Quick-Load® 2X Master Mix with GC Buffer

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OrderPhusion® High-Fidelity PCR Master Mix with GC Buffer

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OrderPhusion® High-Fidelity PCR Master Mix with HF Buffer

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OrderPhusion® Hot Start Flex 2X Master Mix

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OrderQ5® High-Fidelity 2X Master Mix

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OrderQ5® Hot Start High-Fidelity 2X Master Mix

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OrderQuick-Load® Taq 2X Master Mix

DNA Manipulation

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OrderBsu DNA Polymerase, Large Fragment

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OrderSulfolobus DNA Polymerase IV

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OrderDNA Polymerase I (E. coli)

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OrderDNA Polymerase I, Large (Klenow) Fragment

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OrderKlenow Fragment (3'→5' exo-)

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Orderphi29 DNA Polymerase Reaction Buffer

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OrderPreCR® Repair Mix

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OrderQuick Blunting™ Kit

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OrderT4 DNA Polymerase

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OrderT7 DNA Polymerase (unmodified)

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OrderTerminal Transferase

Isothermal Amplification & Strand Displacement

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OrderBst 2.0 WarmStart® DNA Polymerase

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OrderBst 3.0 DNA Polymerase

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OrderBst DNA Polymerase, Full Length

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OrderBst DNA Polymerase, Large Fragment

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OrderIsoAmp® II Universal tHDA Kit

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OrderIsothermal Amplification Buffer II Pack

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OrderIsothermal Amplification Buffer Pack

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OrderKlenow Fragment (3'→5' exo-)

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Orderphi29 DNA Polymerase

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OrderWarmStart® Colorimetric LAMP 2X Master Mix (DNA & RNA)

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OrderWarmStart® LAMP Kit (DNA & RNA)

Nucleotide Solutions

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Order3´-Desthiobiotin-GTP

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Order5-methyl-dCTP

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Order7-deaza-dGTP

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OrderAcyclonucleotide Set

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OrderAdenosine 5'-Triphosphate (ATP)

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OrderdATP Solution

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OrderDeoxynucleotide (dNTP) Solution Mix

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OrderDeoxynucleotide (dNTP) Solution Set

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OrderRibonucleotide Solution Set

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OrderRibonucleotide Solution Mix

cDNA Synthesis & Reverse Transcriptases

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OrderE. coli Poly(A) Polymerase

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OrderAMV Reverse Transcriptase

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OrderDNA Polymerase I (E. coli)

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OrderM13KO7 Helper Phage

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OrderM-MuLV Reverse Transcriptase

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OrderM-MuLV Reverse Transcriptase Reaction Buffer

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OrderNEBNext® Ultra II Non-Directional RNA Second Strand Synthesis Module

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OrderOligo d(T)18 mRNA Primer

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OrderOligo d(T)23 VN

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OrderOneTaq® One-Step RT-PCR Kit

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OrderOneTaq® RT-PCR Kit

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OrderPoly(U) Polymerase

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OrderProtoScript® II First Strand cDNA Synthesis Kit

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OrderProtoScript® II Reverse Transcriptase

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OrderProtoScript® First Strand cDNA Synthesis Kit

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OrderRandom Primer 9

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OrderRNase H

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OrderRNase Inhibitor, Murine

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OrderSP6 RNA Polymerase

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OrderT3 RNA Polymerase

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OrderT4 RNA Ligase 2, truncated KQ

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OrderWarmStart® RTx Reverse Transcriptase

RT-PCR

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OrderAMV Reverse Transcriptase

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OrderM-MuLV Reverse Transcriptase

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OrderM-MuLV Reverse Transcriptase Reaction Buffer

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OrderOligo d(T)18 mRNA Primer

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OrderOligo d(T)23 VN

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OrderOneTaq® One-Step RT-PCR Kit

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OrderOneTaq® RT-PCR Kit

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OrderProtoScript® II First Strand cDNA Synthesis Kit

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OrderProtoScript® II Reverse Transcriptase

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OrderProtoScript® First Strand cDNA Synthesis Kit

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OrderRandom Primer 9

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OrderT4 RNA Ligase 2, truncated KQ

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OrderWarmStart® RTx Reverse Transcriptase

Reaction Buffers

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Orderβ-Nicotinamide adenine dinucleotide (NAD+)

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OrderHemo KlenTaq® Reaction Buffer Pack

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OrderIsothermal Amplification Buffer II Pack

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OrderIsothermal Amplification Buffer Pack

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OrderLongAmp® Taq Reaction Buffer Pack

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OrderMagnesium Chloride (MgCl2) Solution

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OrderMagnesium Sulfate (MgSO4) Solution

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OrderM-MuLV Reverse Transcriptase Reaction Buffer

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OrderOneTaq® GC Reaction Buffer Pack

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OrderOneTaq® Standard Reaction Buffer Pack

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Orderphi29 DNA Polymerase Reaction Buffer

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OrderPhusion® GC Buffer Pack

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OrderPhusion® HF Buffer Pack

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OrderQ5® Reaction Buffer Pack

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OrderStandard Taq (Mg-free) Reaction Buffer Pack

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OrderStandard Taq Reaction Buffer Pack

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OrderThermoPol® II (Mg-free) Reaction Buffer Pack

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OrderThermoPol® Reaction Buffer Pack

Q5® High-Fidelity DNA Polymerases

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OrderNEBNext® High-Fidelity 2X PCR Master Mix

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OrderNEBNext® Q5® Hot Start HiFi PCR Master Mix

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OrderNEBNext® Ultra™ II Q5® Master Mix

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OrderQ5® High-Fidelity 2X Master Mix

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OrderQ5® High-Fidelity DNA Polymerase

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OrderQ5® High-Fidelity PCR Kit

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OrderQ5® Hot Start High-Fidelity DNA Polymerase

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OrderQ5® Hot Start High-Fidelity 2X Master Mix

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OrderQ5® Reaction Buffer Pack

OneTaq® DNA Polymerases

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OrderOneTaq® Quick-Load® 2X Master Mix with GC Buffer

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OrderOneTaq® 2X Master Mix with Standard Buffer

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OrderOneTaq® DNA Polymerase

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OrderOneTaq® GC Reaction Buffer Pack

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OrderOneTaq® Hot Start 2X Master Mix with GC Buffer

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OrderOneTaq® Hot Start 2X Master Mix with Standard Buffer

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OrderOneTaq® Hot Start DNA Polymerase

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OrderOneTaq® Hot Start Quick-Load® 2X Master Mix with Standard Buffer

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OrderOneTaq® Quick-Load® 2X Master Mix with Standard Buffer

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OrderOneTaq® 2X Master Mix with GC Buffer

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OrderOneTaq® Hot Start Quick-Load® 2X Master Mix with GC Buffer

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OrderOneTaq® Standard Reaction Buffer Pack

Taq DNA Polymerases

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OrderTaq 2X Master Mix

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OrderTaq 5X Master Mix

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OrderTaq DNA Polymerase with Standard Taq Buffer

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OrderTaq DNA Polymerase with ThermoPol® Buffer

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OrderTaq PCR Kit

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OrderTaq DNA Polymerase with Standard Taq (Mg-free) Buffer

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OrderEpiMark® Hot Start Taq DNA Polymerase

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OrderHot Start Taq DNA Polymerase

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OrderHot Start Taq 2X Master Mix

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OrderMultiplex PCR 5X Master Mix

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OneTaq Hot Start 2X Master Mix with Standard Buffer

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OrderOneTaq® Quick-Load® 2X Master Mix with GC Buffer

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OrderOneTaq® 2X Master Mix with Standard Buffer

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OrderOneTaq® DNA Polymerase

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OrderOneTaq® GC Reaction Buffer Pack

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OrderOneTaq® Hot Start 2X Master Mix with GC Buffer

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OrderOneTaq® Hot Start 2X Master Mix with Standard Buffer

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OrderOneTaq® Hot Start DNA Polymerase

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OrderOneTaq® Hot Start Quick-Load® 2X Master Mix with Standard Buffer

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OrderOneTaq® Quick-Load® 2X Master Mix with Standard Buffer

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OrderOneTaq® 2X Master Mix with GC Buffer

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OrderOneTaq® Hot Start Quick-Load® 2X Master Mix with GC Buffer

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OrderOneTaq® Standard Reaction Buffer Pack

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OrderQuick-Load® Taq 2X Master Mix

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OrderStandard Taq (Mg-free) Reaction Buffer Pack

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OrderStandard Taq Reaction Buffer Pack

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OrderThermoPol® II (Mg-free) Reaction Buffer Pack

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OrderThermoPol® Reaction Buffer Pack

LongAmp™ DNA Polymerases

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OrderLongAmp® Taq DNA Polymerase

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OrderLongAmp® Taq PCR Kit

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OrderLongAmp® Taq Reaction Buffer Pack

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OrderLongAmp® Taq 2X Master Mix

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OrderLongAmp® Hot Start Taq 2X Master Mix

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OrderLongAmp® Hot Start Taq DNA Polymerase

Luna qPCR & RT-qPCR Products

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OrderLuna® Universal One-Step RT-qPCR Kit

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OrderLuna® Universal Probe One-Step RT-qPCR Kit

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OrderLuna® Universal Probe qPCR Master Mix

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OrderLuna® Universal qPCR Master Mix

Other PCR Polymerases

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OrderDeep Vent® (exo-) DNA Polymerase

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OrderDeep Vent® DNA Polymerase

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OrderHemo KlenTaq®

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OrderVent® (exo-) DNA Polymerase

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OrderVent® DNA Polymerase

Phusion® High-Fidelity DNA Polymerases

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OrderPhusion® GC Buffer Pack

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OrderPhusion® HF Buffer Pack

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OrderPhusion® High-Fidelity DNA Polymerase

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OrderPhusion® High-Fidelity PCR Kit

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OrderPhusion® High-Fidelity PCR Master Mix with GC Buffer

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OrderPhusion® High-Fidelity PCR Master Mix with HF Buffer

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OrderPhusion® Hot Start Flex 2X Master Mix

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OrderPhusion® Hot Start Flex DNA Polymerase

Master Mixes

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OrderTaq 2X Master Mix

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OrderTaq 5X Master Mix

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OrderHot Start Taq 2X Master Mix

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OrderLongAmp® Taq 2X Master Mix

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OrderLongAmp® Hot Start Taq 2X Master Mix

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OrderMultiplex PCR 5X Master Mix

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OrderNEBNext® Q5® Hot Start HiFi PCR Master Mix

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OrderOneTaq® Quick-Load® 2X Master Mix with GC Buffer

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OrderOneTaq® 2X Master Mix with Standard Buffer

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OrderOneTaq® Hot Start 2X Master Mix with GC Buffer

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OrderOneTaq® Hot Start 2X Master Mix with Standard Buffer

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OrderOneTaq® Hot Start Quick-Load® 2X Master Mix with Standard Buffer

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OrderOneTaq® Quick-Load® 2X Master Mix with Standard Buffer

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OrderOneTaq® 2X Master Mix with GC Buffer

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OrderOneTaq® Hot Start Quick-Load® 2X Master Mix with GC Buffer

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OrderPhusion® High-Fidelity PCR Master Mix with GC Buffer

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OrderPhusion® High-Fidelity PCR Master Mix with HF Buffer

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OrderPhusion® Hot Start Flex 2X Master Mix

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OrderQ5® High-Fidelity 2X Master Mix

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OrderQ5® Hot Start High-Fidelity 2X Master Mix

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OrderQuick-Load® Taq 2X Master Mix

DNA Manipulation

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OrderBsu DNA Polymerase, Large Fragment

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OrderSulfolobus DNA Polymerase IV

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OrderDNA Polymerase I (E. coli)

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OrderDNA Polymerase I, Large (Klenow) Fragment

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OrderKlenow Fragment (3'→5' exo-)

Close

Orderphi29 DNA Polymerase Reaction Buffer

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OrderPreCR® Repair Mix

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OrderQuick Blunting™ Kit

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OrderT4 DNA Polymerase

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OrderT7 DNA Polymerase (unmodified)

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OrderTerminal Transferase

Isothermal Amplification & Strand Displacement

Close

OrderBst 2.0 WarmStart® DNA Polymerase

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OrderBst 3.0 DNA Polymerase

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OrderBst DNA Polymerase, Full Length

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OrderBst DNA Polymerase, Large Fragment

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OrderIsoAmp® II Universal tHDA Kit

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OrderIsothermal Amplification Buffer II Pack

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OrderIsothermal Amplification Buffer Pack

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OrderKlenow Fragment (3'→5' exo-)

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Orderphi29 DNA Polymerase

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OrderWarmStart® Colorimetric LAMP 2X Master Mix (DNA & RNA)

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OrderWarmStart® LAMP Kit (DNA & RNA)

Nucleotide Solutions

Close

Order3´-Desthiobiotin-GTP

Close

Order5-methyl-dCTP

Close

Order7-deaza-dGTP

Close

OrderAcyclonucleotide Set

Close

OrderAdenosine 5'-Triphosphate (ATP)

Close

OrderdATP Solution

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OrderDeoxynucleotide (dNTP) Solution Mix

Close

OrderDeoxynucleotide (dNTP) Solution Set

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OrderRibonucleotide Solution Set

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OrderRibonucleotide Solution Mix

cDNA Synthesis & Reverse Transcriptases

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OrderE. coli Poly(A) Polymerase

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OrderAMV Reverse Transcriptase

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OrderDNA Polymerase I (E. coli)

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OrderM13KO7 Helper Phage

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OrderM-MuLV Reverse Transcriptase

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OrderM-MuLV Reverse Transcriptase Reaction Buffer

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OrderNEBNext® Ultra II Non-Directional RNA Second Strand Synthesis Module

Close

OrderOligo d(T)18 mRNA Primer

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OrderOligo d(T)23 VN

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OrderOneTaq® One-Step RT-PCR Kit

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OrderOneTaq® RT-PCR Kit

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OrderPoly(U) Polymerase

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OrderProtoScript® II First Strand cDNA Synthesis Kit

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OrderProtoScript® II Reverse Transcriptase

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OrderProtoScript® First Strand cDNA Synthesis Kit

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OrderRandom Primer 9

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OrderRNase H

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OrderRNase Inhibitor, Murine

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OrderSP6 RNA Polymerase

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OrderT3 RNA Polymerase

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OrderT4 RNA Ligase 2, truncated KQ

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OrderWarmStart® RTx Reverse Transcriptase

RT-PCR

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OrderAMV Reverse Transcriptase

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OrderM-MuLV Reverse Transcriptase

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OrderM-MuLV Reverse Transcriptase Reaction Buffer

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OrderOligo d(T)18 mRNA Primer

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OrderOligo d(T)23 VN

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OrderOneTaq® One-Step RT-PCR Kit

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OrderOneTaq® RT-PCR Kit

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OrderProtoScript® II First Strand cDNA Synthesis Kit

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OrderProtoScript® II Reverse Transcriptase

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OrderProtoScript® First Strand cDNA Synthesis Kit

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OrderRandom Primer 9

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OrderT4 RNA Ligase 2, truncated KQ

Close

OrderWarmStart® RTx Reverse Transcriptase

Reaction Buffers

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Orderβ-Nicotinamide adenine dinucleotide (NAD+)

Close

OrderHemo KlenTaq® Reaction Buffer Pack

Close

OrderIsothermal Amplification Buffer II Pack

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OrderIsothermal Amplification Buffer Pack

Close

OrderLongAmp® Taq Reaction Buffer Pack

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OrderMagnesium Chloride (MgCl2) Solution

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OrderMagnesium Sulfate (MgSO4) Solution

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OrderM-MuLV Reverse Transcriptase Reaction Buffer

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OrderOneTaq® GC Reaction Buffer Pack

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OrderOneTaq® Standard Reaction Buffer Pack

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Orderphi29 DNA Polymerase Reaction Buffer

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OrderPhusion® GC Buffer Pack

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OrderPhusion® HF Buffer Pack

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OrderQ5® Reaction Buffer Pack

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OrderStandard Taq (Mg-free) Reaction Buffer Pack

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OrderStandard Taq Reaction Buffer Pack

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OrderThermoPol® II (Mg-free) Reaction Buffer Pack

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OrderThermoPol® Reaction Buffer Pack

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