Polymerases and Amplification Technologies

PCR, qPCR & Amplification Technologies

NEB offers a wide range of DNA polymerases, and through our commitment to research, ensures the development of innovative, high quality tools for DNA amplification, qPCR and PCR. Our amplification technologies product portfolio includes a broad variety of both DNA and RNA polymerases, nucleotide mixes, kits, master mixes and more, featuring:

  1. Overview of PCR

    This overview will walk you through how the Polymerase Chain Reaction (PCR) works.

  2. Overview of qPCR

    Learn the basics of qPCR in this short animation.                                                                                                  

  3. “Dots in Boxes" Visualization of qPCR Data

    Learn how we analyzed plates and plates of qPCR data, and how Luna stacks up to the competition.

PCR, qPCR & Amplification Technologies includes these subcategories:

Q5® High-Fidelity DNA Polymerases
OneTaq® DNA Polymerases
Taq DNA Polymerases
LongAmp® DNA Polymerases
Luna qPCR & RT-qPCR Products
Other PCR Polymerases
Phusion® High-Fidelity DNA Polymerases
Master Mixes Information
DNA Manipulation
Isothermal Amplification & Strand Displacement
Nucleotide Solutions Information
cDNA Synthesis & Reverse Transcriptases
RT-PCR Information

DNA Polymerase Selection Chart

NEB offers a guidelines for choosing the correct DNA polymerase for your application by providing a list of specific properites.
Several factors govern which polymerase should be used in a given application, including:
Template/product specificity: Is RNA or DNA involved? Is the 3´ terminus at a gap, nick or at the end of the template?
Removal of existing nucleotides: Will the nucleotide(s) be removed from the existing polynucleotide chain as part of the protocol? If so, will they be removed from the 5´ or the 3´ end?
Thermal stability: Does the polymerase need to survive incubation at high temperature or is heat inactivation desirable?
Fidelity: Will subsequent sequence analysis or expression depend on the fidelity of the synthesized products?

PCR Selection Tool

Choose from one of the largest selections of polymerases for PCR applications from the leader in enzyme technology and bring unparalleled confidence to your experiments.