Protein Expression

Protein Expression & Purification Technologies

Protein expression and purification can be very challenging because there is no single approach that is appropriate for every protein or downstream application.

Successful expression can be affected by each individual protein’s solubility, toxicity, need for post-translational modifications or its intrinsic ability to fold. Additionally, the application for which the protein is ultimately to be used may dictate how the protein needs to be expressed or purified. It is therefore important to have a flexible set of tools and methods that can be applied to each unique project.

Each NEB expression technology offers different advantages enabling you to choose the strategy that best suits your protein expression and purification needs. Many of the expression vectors share a compatible polylinker, enabling the gene of interest to be easily shuffled between systems. Various applications along with the recommended NEB kit can be found by clicking the sidebar tab.

Solutions for the Expression of Difficult Proteins

NEB has a long history in recombinant protein expression and has developed a wide array of solutions for proteins that are difficult to express.

NEB has a long history in recombinant protein expression and has developed a wide array of solutions for proteins that are difficult to express.

Featured Products

Protein Expression & Purification Technologies includes these subcategories:

Cell-Free Expression
E. coli
SHuffle strains for the expression of multi-disulfide bonded and difficult-to-express proteins
Magnetic Matrices and Racks
Yeast

Protein Expression and Purification: Selection Chart

Each NEB expression technology offers different advantages which enable you to choose the strategy that best suits your protein expression and purification needs.