Restriction Endonucleases

Restriction Endonucleases

Cut Smarter with Restriction Enzymes from NEB

With over 40 years of offering restriction enzymes to the research community, NEB has earned the reputation of being a leader in enzyme technologies. Working continuously to be worthy of that distinction, NEB strives to develop enzymes of the highest purity and unparalleled performance.

All of NEB's Restriction enzymes have transitioned to a new buffer system. Visit NEBCutSmart.com for further details. 

Convenience


Performance

  • Choose a High-Fidelity (HF®) restriction enzyme, which has been engineered for reduced star activity, rapid digestion (5-15 minutes) and 100% activity in CutSmart Buffer. A vial of 6X Purple Load Dye is included with every HF restriction enzyme.
  • All of our restriction enzymes undergo stringent quality control testing, ensuring the highest levels of purity and lot-to-lot consistency.

Use Enzyme Finder to select restriction enzymes by name, sequence, overhang or type.

  1. Type II Restriction Enzymes

    Type II restriction enzymes are most commonly used for molecular biology applications, as they recognize stereotypical sequences and produce a predictable cleavage pattern. Learn more about how Type II REs work.

  2. Type I Restriction Enzymes

    Type I restriction enzymes are a group of endonucleases that recognize a bipartite sequence, but do not produce a predictable cleavage pattern. Learn more about how Type I REs work.

  3. Type III Restriction Enzymes

    Type III restriction enzymes are a group of endonucleases that recognize a non-pallindromic sequence, comprising two inversely oriented sites. Learn more about these poorly understood enzymes.

Restriction Endonucleases

Restriction Endonucleases: A

Close

AatII

Close

AbaSI

Close

Acc65I

Close

AccI

Close

AciI

Close

AclI

Close

AcuI

Close

AfeI

Close

AflII

Close

AflIII

Close

AgeI

Close

AgeI-HF®

Close

AhdI

Close

AleI

Close

AluI

Close

AlwI

Close

AlwNI

Close

ApaI

Close

ApaLI

Close

ApeKI

Close

ApoI

Close

ApoI-HF®

Close

AscI

Close

AseI

Close

AsiSI

Close

AvaI

Close

AvaII

Close

AvrII

Restriction Endonucleases: B

Close

BaeGI

Close

BaeI

Close

BamHI

Close

BamHI-HF®

Close

BanI

Close

BanII

Close

BbsI

Close

BbsI-HF®

Close

BbvCI

Close

BbvI

Close

BccI

Close

BceAI

Close

BcgI

Close

BciVI

Close

BclI

Close

BclI-HF

Close

BcoDI

Close

BfaI

Close

BfuAI

Close

BglI

Close

BglII

Close

BlpI

Close

BmgBI

Close

BmrI

Close

BmtI

Close

BmtI-HF®

Close

BpmI

Close

Bpu10I

Close

BpuEI

Close

BsaAI

Close

BsaBI

Close

BsaHI

Close

BsaI

Close

BsaI-HF®

Close

BsaJI

Close

BsaWI

Close

BsaXI

Close

BseRI

Close

BseYI

Close

BsgI

Close

BsiEI

Close

BsiHKAI

Close

BsiWI

Close

BsiWI-HF®

Close

BslI

Close

BsmAI

Close

BsmBI

Close

BsmFI

Close

BsmI

Close

BsoBI

Close

Bsp1286I

Close

BspCNI

Close

BspDI

Close

BspEI

Close

BspHI

Close

BspMI

Close

BspQI

Close

BsrBI

Close

BsrDI

Close

BsrFαI

Close

BsrGI

Close

BsrGI-HF®

Close

BsrI

Close

BssHII

Close

BssSαI

Close

BstAPI

Close

BstBI

Close

BstEII

Close

BstEII-HF®

Close

BstNI

Close

BstUI

Close

BstXI

Close

BstYI

Close

BstZ17I-HF®

Close

Bsu36I

Close

BtgI

Close

BtgZI

Close

BtsαI

Close

BtsCI

Close

BtsIMutI

Restriction Endonucleases: C-G

Close

Cac8I

Close

ClaI

Close

CspCI

Close

CviAII

Close

CviKI-1

Close

CviQI

Close

DdeI

Close

DpnI

Close

DpnII

Close

DraI

Close

DraIII-HF®

Close

DrdI

Close

EaeI

Close

EagI

Close

EagI-HF®

Close

EarI

Close

EciI

Close

Eco53kI

Close

EcoNI

Close

EcoO109I

Close

EcoP15I

Close

EcoRI

Close

EcoRI-HF®

Close

EcoRV

Close

EcoRV-HF®

Close

FatI

Close

FauI

Close

Fnu4HI

Close

FokI

Close

FseI

Close

FspEI

Close

FspI

Restriction Endonucleases: H-M

Close

HaeII

Close

HaeIII

Close

HgaI

Close

HhaI

Close

HincII

Close

HindIII

Close

HindIII-HF®

Close

HinfI

Close

HinP1I

Close

HpaI

Close

HpaII

Close

HphI

Close

Hpy166II

Close

Hpy188I

Close

Hpy188III

Close

Hpy99I

Close

HpyAV

Close

HpyCH4III

Close

HpyCH4IV

Close

HpyCH4V

Close

I-CeuI

Close

I-SceI

Close

KasI

Close

KpnI

Close

KpnI-HF®

Close

LpnPI

Close

MboI

Close

MboII

Close

MfeI

Close

MfeI-HF®

Close

MluCI

Close

MluI

Close

MluI-HF®

Close

MlyI

Close

MmeI

Close

MnlI

Close

MscI

Close

MseI

Close

MslI

Close

MspA1I

Close

MspI

Close

MspJI

Close

MwoI

Restriction Endonucleases: N-O

Close

NaeI

Close

NarI

Close

Nb.BbvCI

Close

Nb.BsmI

Close

Nb.BsrDI

Close

Nb.BssSI

Close

Nb.BtsI

Close

NciI

Close

NcoI

Close

NcoI-HF®

Close

NdeI

Close

NgoMIV

Close

NheI

Close

NheI-HF®

Close

NlaIII

Close

NlaIV

Close

NmeAIII

Close

NotI

Close

NotI-HF®

Close

NruI

Close

NruI-HF®

Close

NsiI

Close

NsiI-HF®

Close

NspI

Close

Nt.AlwI

Close

Nt.BbvCI

Close

Nt.BsmAI

Close

Nt.BspQI

Close

Nt.BstNBI

Restriction Endonucleases: P-R

Close

PacI

Close

PaeR7I

Close

PciI

Close

PflFI

Close

PflMI

Close

PI-PspI

Close

PI-SceI

Close

PleI

Close

PluTI

Close

PmeI

Close

PmlI

Close

PpuMI

Close

PshAI

Close

PsiI

Close

PspGI

Close

PspOMI

Close

PspXI

Close

PstI

Close

PstI-HF®

Close

PvuI

Close

PvuI-HF®

Close

PvuII

Close

PvuII-HF®

Close

RsaI

Close

RsrII

Restriction Endonucleases: S

Close

SacI

Close

SacI-HF®

Close

SacII

Close

SalI

Close

SalI-HF®

Close

SapI

Close

Sau3AI

Close

Sau96I

Close

SbfI

Close

SbfI-HF®

Close

ScaI-HF®

Close

ScrFI

Close

SexAI

Close

SfaNI

Close

SfcI

Close

SfiI

Close

SfoI

Close

SgrAI

Close

SmaI

Close

SmlI

Close

SnaBI

Close

SpeI

Close

SpeI-HF®

Close

SphI

Close

SphI-HF®

Close

SrfI

Close

SspI-HF®

Close

StuI

Close

StyD4I

Close

StyI

Close

StyI-HF®

Close

SwaI

Restriction Endonucleases: T-Z

Close

TaqαI

Close

TfiI

Close

TseI

Close

Tsp45I

Close

TspMI

Close

TspRI

Close

Tth111I

Close

XbaI

Close

XcmI

Close

XhoI

Close

XmaI

Close

XmnI

Close

ZraI

HF®, Nicking, Time-Saver & Other

Close

AatII

Close

AbaSI

Close

Acc65I

Close

AccI

Close

AciI

Close

AclI

Close

AcuI

Close

AflII

Close

AgeI-HF®

Close

AhdI

Close

AluI

Close

AlwNI

Close

ApaI

Close

ApaLI

Close

ApeKI

Close

ApoI

Close

ApoI-HF®

Close

AscI

Close

AseI

Close

AvaI

Close

AvaII

Close

AvrII

Close

BaeGI

Close

BaeI

Close

BamHI

Close

BamHI-HF®

Close

BbsI

Close

BbsI-HF®

Close

BbvI

Close

BciVI

Close

BclI

Close

BclI-HF

Close

BcoDI

Close

BfuAI

Close

BglI

Close

BglII

Close

BlpI

Close

BmgBI

Close

BmtI-HF®

Close

BpmI

Close

BpuEI

Close

BsaAI

Close

BsaHI

Close

BsaI-HF®

Close

BsaWI

Close

BsaXI

Close

BseRI

Close

BsgI

Close

BsiEI

Close

BsiWI

Close

BsiWI-HF®

Close

BslI

Close

BsmAI

Close

BsmBI

Close

BsmI

Close

BsoBI

Close

Bsp1286I

Close

BspCNI

Close

BspEI

Close

BspHI

Close

BspQI

Close

BsrBI

Close

BsrDI

Close

BsrGI

Close

BsrGI-HF®

Close

BsrI

Close

BssHII

Close

BstBI

Close

BstEII

Close

BstEII-HF®

Close

BstNI

Close

BstUI

Close

BstXI

Close

BstYI

Close

BstZ17I-HF®

Close

Bsu36I

Close

BtgI

Close

BtsCI

Close

Cac8I

Close

ClaI

Close

CspCI

Close

CviAII

Close

CviQI

Close

DdeI

Close

DpnI

Close

DpnII

Close

DraI

Close

DraIII-HF®

Close

DrdI

Close

EagI

Close

EagI-HF®

Close

EarI

Close

Eco53kI

Close

EcoNI

Close

EcoO109I

Close

EcoP15I

Close

EcoRI

Close

EcoRI-HF®

Close

EcoRV

Close

EcoRV-HF®

Close

Fnu4HI

Close

FseI

Close

FspEI

Close

FspI

Close

HaeIII

Close

HhaI

Close

HincII

Close

HindIII-HF®

Close

HinfI

Close

HinP1I

Close

HpaII

Close

HphI

Close

Hpy166II

Close

HpyAV

Close

HpyCH4IV

Close

HpyCH4V

Close

I-CeuI

Close

I-SceI

Close

KpnI

Close

KpnI-HF®

Close

LpnPI

Close

MboI

Close

McrBC

Close

MfeI-HF®

Close

MluCI

Close

MluI

Close

MluI-HF®

Close

MlyI

Close

MmeI

Close

MnlI

Close

MseI

Close

MslI

Close

MspA1I

Close

MspI

Close

MspJI

Close

MwoI

Close

Nb.BbvCI

Close

Nb.BsmI

Close

Nb.BsrDI

Close

Nb.BssSI

Close

Nb.BtsI

Close

NciI

Close

NcoI

Close

NcoI-HF®

Close

NdeI

Close

NgoMIV

Close

NheI

Close

NheI-HF®

Close

NlaIII

Close

NotI

Close

NotI-HF®

Close

NruI

Close

NruI-HF®

Close

NsiI

Close

NsiI-HF®

Close

NspI

Close

Nt.AlwI

Close

Nt.BbvCI

Close

Nt.BsmAI

Close

Nt.BspQI

Close

Nt.BstNBI

Close

PacI

Close

PaeR7I

Close

PflFI

Close

PflMI

Close

PI-PspI

Close

PI-SceI

Close

PmeI

Close

PmlI

Close

PpuMI

Close

PshAI

Close

PstI

Close

PstI-HF®

Close

PvuI

Close

PvuI-HF®

Close

PvuII

Close

PvuII-HF®

Close

RsaI

Close

SacI

Close

SacI-HF®

Close

SacII

Close

SalI

Close

SalI-HF®

Close

SapI

Close

SbfI

Close

SbfI-HF®

Close

ScaI-HF®

Close

SfiI

Close

SfoI

Close

SmaI

Close

SpeI

Close

SpeI-HF®

Close

SphI-HF®

Close

SspI-HF®

Close

StuI

Close

StyD4I

Close

StyI

Close

StyI-HF®

Close

SwaI

Close

TaqαI

Close

TfiI

Close

TseI

Close

TspMI

Close

TspRI

Close

Tth111I

Close

XbaI

Close

XhoI

Close

XmaI

Close

XmnI

Restriction Endonuclease Buffers & Diluents

Close

BSA, Molecular Biology Grade

Close

CutSmart® Buffer

Close

Diluent A

Close

Diluent B

Close

Diluent C

Close

NEBuffer™ 1

Close

NEBuffer™ 1.1

Close

NEBuffer™ 2

Close

NEBuffer™ 2.1

Close

NEBuffer™ 3

Close

NEBuffer™ 3.1

Close

NEBuffer™ 4

Close

NEBuffer™ EcoRI

Close

NEBuffer™ Set (1.1, 2.1, 3.1 and CutSmart®)

Close

S-adenosylmethionine (SAM)

Restriction Endonucleases: A

Close

AatII

Close

AbaSI

Close

Acc65I

Close

AccI

Close

AciI

Close

AclI

Close

AcuI

Close

AfeI

Close

AflII

Close

AflIII

Close

AgeI

Close

AgeI-HF®

Close

AhdI

Close

AleI

Close

AluI

Close

AlwI

Close

AlwNI

Close

ApaI

Close

ApaLI

Close

ApeKI

Close

ApoI

Close

ApoI-HF®

Close

AscI

Close

AseI

Close

AsiSI

Close

AvaI

Close

AvaII

Close

AvrII

Restriction Endonucleases: B

Close

BaeGI

Close

BaeI

Close

BamHI

Close

BamHI-HF®

Close

BanI

Close

BanII

Close

BbsI

Close

BbsI-HF®

Close

BbvCI

Close

BbvI

Close

BccI

Close

BceAI

Close

BcgI

Close

BciVI

Close

BclI

Close

BclI-HF

Close

BcoDI

Close

BfaI

Close

BfuAI

Close

BglI

Close

BglII

Close

BlpI

Close

BmgBI

Close

BmrI

Close

BmtI

Close

BmtI-HF®

Close

BpmI

Close

Bpu10I

Close

BpuEI

Close

BsaAI

Close

BsaBI

Close

BsaHI

Close

BsaI

Close

BsaI-HF®

Close

BsaJI

Close

BsaWI

Close

BsaXI

Close

BseRI

Close

BseYI

Close

BsgI

Close

BsiEI

Close

BsiHKAI

Close

BsiWI

Close

BsiWI-HF®

Close

BslI

Close

BsmAI

Close

BsmBI

Close

BsmFI

Close

BsmI

Close

BsoBI

Close

Bsp1286I

Close

BspCNI

Close

BspDI

Close

BspEI

Close

BspHI

Close

BspMI

Close

BspQI

Close

BsrBI

Close

BsrDI

Close

BsrFαI

Close

BsrGI

Close

BsrGI-HF®

Close

BsrI

Close

BssHII

Close

BssSαI

Close

BstAPI

Close

BstBI

Close

BstEII

Close

BstEII-HF®

Close

BstNI

Close

BstUI

Close

BstXI

Close

BstYI

Close

BstZ17I-HF®

Close

Bsu36I

Close

BtgI

Close

BtgZI

Close

BtsαI

Close

BtsCI

Close

BtsIMutI

Restriction Endonucleases: C-G

Close

Cac8I

Close

ClaI

Close

CspCI

Close

CviAII

Close

CviKI-1

Close

CviQI

Close

DdeI

Close

DpnI

Close

DpnII

Close

DraI

Close

DraIII-HF®

Close

DrdI

Close

EaeI

Close

EagI

Close

EagI-HF®

Close

EarI

Close

EciI

Close

Eco53kI

Close

EcoNI

Close

EcoO109I

Close

EcoP15I

Close

EcoRI

Close

EcoRI-HF®

Close

EcoRV

Close

EcoRV-HF®

Close

FatI

Close

FauI

Close

Fnu4HI

Close

FokI

Close

FseI

Close

FspEI

Close

FspI

Restriction Endonucleases: H-M

Close

HaeII

Close

HaeIII

Close

HgaI

Close

HhaI

Close

HincII

Close

HindIII

Close

HindIII-HF®

Close

HinfI

Close

HinP1I

Close

HpaI

Close

HpaII

Close

HphI

Close

Hpy166II

Close

Hpy188I

Close

Hpy188III

Close

Hpy99I

Close

HpyAV

Close

HpyCH4III

Close

HpyCH4IV

Close

HpyCH4V

Close

I-CeuI

Close

I-SceI

Close

KasI

Close

KpnI

Close

KpnI-HF®

Close

LpnPI

Close

MboI

Close

MboII

Close

MfeI

Close

MfeI-HF®

Close

MluCI

Close

MluI

Close

MluI-HF®

Close

MlyI

Close

MmeI

Close

MnlI

Close

MscI

Close

MseI

Close

MslI

Close

MspA1I

Close

MspI

Close

MspJI

Close

MwoI

Restriction Endonucleases: N-O

Close

NaeI

Close

NarI

Close

Nb.BbvCI

Close

Nb.BsmI

Close

Nb.BsrDI

Close

Nb.BssSI

Close

Nb.BtsI

Close

NciI

Close

NcoI

Close

NcoI-HF®

Close

NdeI

Close

NgoMIV

Close

NheI

Close

NheI-HF®

Close

NlaIII

Close

NlaIV

Close

NmeAIII

Close

NotI

Close

NotI-HF®

Close

NruI

Close

NruI-HF®

Close

NsiI

Close

NsiI-HF®

Close

NspI

Close

Nt.AlwI

Close

Nt.BbvCI

Close

Nt.BsmAI

Close

Nt.BspQI

Close

Nt.BstNBI

Restriction Endonucleases: P-R

Close

PacI

Close

PaeR7I

Close

PciI

Close

PflFI

Close

PflMI

Close

PI-PspI

Close

PI-SceI

Close

PleI

Close

PluTI

Close

PmeI

Close

PmlI

Close

PpuMI

Close

PshAI

Close

PsiI

Close

PspGI

Close

PspOMI

Close

PspXI

Close

PstI

Close

PstI-HF®

Close

PvuI

Close

PvuI-HF®

Close

PvuII

Close

PvuII-HF®

Close

RsaI

Close

RsrII

Restriction Endonucleases: S

Close

SacI

Close

SacI-HF®

Close

SacII

Close

SalI

Close

SalI-HF®

Close

SapI

Close

Sau3AI

Close

Sau96I

Close

SbfI

Close

SbfI-HF®

Close

ScaI-HF®

Close

ScrFI

Close

SexAI

Close

SfaNI

Close

SfcI

Close

SfiI

Close

SfoI

Close

SgrAI

Close

SmaI

Close

SmlI

Close

SnaBI

Close

SpeI

Close

SpeI-HF®

Close

SphI

Close

SphI-HF®

Close

SrfI

Close

SspI-HF®

Close

StuI

Close

StyD4I

Close

StyI

Close

StyI-HF®

Close

SwaI

Restriction Endonucleases: T-Z

Close

TaqαI

Close

TfiI

Close

TseI

Close

Tsp45I

Close

TspMI

Close

TspRI

Close

Tth111I

Close

XbaI

Close

XcmI

Close

XhoI

Close

XmaI

Close

XmnI

Close

ZraI

HF®, Nicking, Time-Saver & Other

High-Fidelity (HF®) Restriction Endonucleases

Close

AgeI-HF®

Close

ApoI-HF®

Close

BamHI-HF®

Close

BbsI-HF®

Close

BclI-HF

Close

BmtI-HF®

Close

BsaI-HF®

Close

BsiWI-HF®

Close

BsrGI-HF®

Close

BstEII-HF®

Close

BstZ17I-HF®

Close

DraIII-HF®

Close

EagI-HF®

Close

EcoRI-HF®

Close

EcoRV-HF®

Close

HindIII-HF®

Close

KpnI-HF®

Close

MfeI-HF®

Close

MluI-HF®

Close

NcoI-HF®

Close

NheI-HF®

Close

NotI-HF®

Close

NruI-HF®

Close

NsiI-HF®

Close

PstI-HF®

Close

PvuI-HF®

Close

PvuII-HF®

Close

SacI-HF®

Close

SalI-HF®

Close

SbfI-HF®

Close

ScaI-HF®

Close

SpeI-HF®

Close

SphI-HF®

Close

SspI-HF®

Close

StyI-HF®

Homing Endonucleases

Close

I-CeuI

Close

I-SceI

Close

PI-PspI

Close

PI-SceI

Nicking Endonucleases

Close

Nb.BbvCI

Close

Nb.BsmI

Close

Nb.BsrDI

Close

Nb.BssSI

Close

Nb.BtsI

Close

Nt.AlwI

Close

Nt.BbvCI

Close

Nt.BsmAI

Close

Nt.BspQI

Close

Nt.BstNBI

Restriction Enzymes for Epigenetic Analysis (EpiMark® Validated)

Close

AbaSI

Close

DpnI

Close

DpnII

Close

FspEI

Close

HpaII

Close

LpnPI

Close

McrBC

Close

MspI

Close

MspJI

Time-Saver™ Qualified Restriction Enzymes

Close

AatII

Close

Acc65I

Close

AccI

Close

AciI

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AclI

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AcuI

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AflII

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AgeI-HF®

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AhdI

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AluI

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AlwNI

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ApaI

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ApaLI

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ApeKI

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ApoI

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ApoI-HF®

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AscI

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AseI

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AvaI

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AvaII

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AvrII

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BaeGI

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BaeI

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BamHI

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BamHI-HF®

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BbsI

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BbsI-HF®

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BbvI

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BciVI

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BclI

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BclI-HF

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BcoDI

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BfuAI

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BglI

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BglII

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BlpI

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BmgBI

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BmtI-HF®

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BpmI

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BpuEI

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BsaAI

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BsaHI

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BsaI-HF®

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BsaWI

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BsaXI

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BseRI

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BsgI

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BsiEI

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BsiWI

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BsiWI-HF®

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BslI

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BsmAI

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BsmBI

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BsmI

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BsoBI

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Bsp1286I

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BspCNI

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BspEI

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BspHI

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BspQI

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BsrBI

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BsrDI

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BsrGI

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BsrI

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BssHII

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BstBI

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BstEII

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BstEII-HF®

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BstNI

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BstUI

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BstXI

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BstYI

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BstZ17I-HF®

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Bsu36I

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BtgI

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BtsCI

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Cac8I

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ClaI

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CspCI

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CviAII

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CviQI

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DdeI

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DpnI

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DpnII

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DraI

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DraIII-HF®

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DrdI

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EagI

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EagI-HF®

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EarI

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Eco53kI

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EcoNI

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EcoO109I

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EcoP15I

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EcoRI

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EcoRI-HF®

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EcoRV

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EcoRV-HF®

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Fnu4HI

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FseI

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FspI

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HaeIII

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HhaI

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HincII

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HindIII-HF®

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HinfI

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HinP1I

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HpaII

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HphI

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Hpy166II

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HpyAV

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HpyCH4IV

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HpyCH4V

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KpnI

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KpnI-HF®

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MboI

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MfeI-HF®

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MluCI

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MluI

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MlyI

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MmeI

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MnlI

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MseI

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MslI

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MspA1I

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MspI

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MwoI

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NciI

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NcoI

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NcoI-HF®

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NdeI

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NgoMIV

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NheI

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NheI-HF®

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NlaIII

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NotI

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NotI-HF®

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NruI

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NsiI

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NspI

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PacI

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PaeR7I

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PflFI

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PflMI

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PmeI

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PmlI

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PpuMI

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PshAI

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PstI

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PstI-HF®

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PvuI

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PvuI-HF®

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PvuII

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PvuII-HF®

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RsaI

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SacI

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SacI-HF®

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SacII

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SalI

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SalI-HF®

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SapI

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SbfI

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SbfI-HF®

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ScaI-HF®

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SfiI

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SfoI

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SmaI

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SpeI

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SpeI-HF®

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SphI-HF®

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SspI-HF®

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StuI

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StyD4I

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StyI

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StyI-HF®

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SwaI

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TaqαI

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TfiI

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TseI

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TspMI

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TspRI

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Tth111I

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XbaI

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XhoI

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XmaI

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XmnI

Restriction Endonuclease Buffers & Diluents

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BSA, Molecular Biology Grade

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CutSmart® Buffer

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Diluent A

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Diluent B

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Diluent C

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NEBuffer™ 1

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NEBuffer™ 1.1

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NEBuffer™ 2

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NEBuffer™ 2.1

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NEBuffer™ 3

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NEBuffer™ 3.1

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NEBuffer™ 4

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NEBuffer™ EcoRI

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NEBuffer™ Set (1.1, 2.1, 3.1 and CutSmart®)

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S-adenosylmethionine (SAM)

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