- Dilute up to 1 μg DNA to 36 μl with dH20
- Add 2 μl of each 10X RE-Mix
- Incubate for 15 minutes at 37°C
- Analyze by agarose gel electrophoresis
Notes: Alternatively, double digest can be carried out in a 20 μl volume using 1 μl of each RE-Mix and a minimum incubation time of 30 minutes.
There is also a protocol
for double digest with a standard restriction enzyme with RE-Mix.