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Buffer and Diluent Formulation Table

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  • The following chart lists the components of various common buffers and diluents from New England Biolabs.

    Components NEBuffer 1.1
    B7201S
    NEBuffer 2.1 B7202S NEBuffer 3.1 B7203S CutSmart Buffer
    B7204S
    T4 DNA Ligase Reaction Buffer
    B0202S
    Quick Ligation Reaction Buffer
    B6058S
    Thermopol Reaction Buffer Pack
    B9004S
    T4 Polynucleotide Kinase Buffer B0201S NEBuffer EcoRI
    B0101S 
    Antarctic Phosphatase Reaction Buffer
    B0289S
    Exonuclease I Reaction Buffer
    B0293S
    Exonuclease VII Reaction Buffer Diluent A |
    B8001S
    Diluent B
    B8002S
    Diluent C
    B8003S
    Tris-HCl 
    10mM  50mM 
    50mM  66mM  20mM  70mM  100mM 

    50 mM 10mM 10mM  10mM 
    Bis-Tris-Propane-HCl  10mM 







    50mM 




    Tris-acetate 


    20mM 










    Sodium Phosphate










    50 mM


    Potassium Acetate 


    50mM 










    Magnesium Acetate 


    10mM 










    EDTA 










    8 mM 0.1mM  0.1mM  0.1mM 
    NaCl 
    50mM  100mM 




    50mM 



    300mM  250mM 
    KCl 





    10mM 




    50mM 

    MgCl2  10mM 10mM  10mM 
    10mM 10mM 
    10mM  10mM  1mM  6.7mM 



    ZnCl2 








    0.1mM 




    MgSO4 





    2mM 







    (NH4)2SO4





    10mM 







    Glycine-KOH









    67mM 



    BSA  100μg/ml 100μg/ml 100μg/ml 100μg/ml







    200μg/ml 500μg/ml 200μg/ml
    ATP 



    1mM  1mM 








    DTT 




    1mM 
    5mM



    1mM  1mM  1mM 
    β-ME 









    10mM 



    2-mercapto-ethanol










    10 mM


    Polyethylene glycol (PEG 6000) 




    6%








    Triton® X-100 





    0.10%
    0.025%




    0.15%
    Glycerol











    50% 50% 50%
    pH @ 25 7.0 7.9 7.9 7.9 7.5 7.6 8.8 7.6 7.5 6.0 9.5 8 7.4 7.4 7.4