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Buffer and Diluent Formulation Table

The following chart lists the components of various common buffers and diluents from New England Biolabs.

Components NEBuffer 1.1
B7201S
NEBuffer 2.1 B7202S NEBuffer 3.1 B7203S CutSmart Buffer
B7204S
T4 DNA Ligase Reaction Buffer
B0202S
Quick Ligation Reaction Buffer
B6058S
Thermopol Reaction Buffer Pack
B9004S
T4 Polynucleotide Kinase Buffer B0201S NEBuffer EcoRI
B0101S 
Antarctic Phosphatase Reaction Buffer
B0289S
Exonuclease I Reaction Buffer
B0293S
Exonuclease VII Reaction Buffer Diluent A |
B8001S
Diluent B
B8002S
Diluent C
B8003S
Tris-HCl 
10mM  50mM 
50mM  66mM  20mM  70mM  100mM 

50 mM 10mM 10mM  10mM 
Bis-Tris-Propane-HCl  10mM 







50mM 




Tris-acetate 


20mM 










Sodium Phosphate










50 mM


Potassium Acetate 


50mM 










Magnesium Acetate 


10mM 










EDTA 










8 mM 0.1mM  0.1mM  0.1mM 
NaCl 
50mM  100mM 




50mM 



300mM  250mM 
KCl 





10mM 




50mM 

MgCl2  10mM 10mM  10mM 
10mM 10mM 
10mM  10mM  1mM  6.7mM 



ZnCl2 








0.1mM 




MgSO4 





2mM 







(NH4)2SO4





10mM 







Glycine-KOH









67mM 



BSA  100μg/ml 100μg/ml 100μg/ml 100μg/ml







200μg/ml 500μg/ml 200μg/ml
ATP 



1mM  1mM 








DTT 




1mM 
5mM



1mM  1mM  1mM 
β-ME 









10mM 



2-mercapto-ethanol










10 mM


Polyethylene glycol (PEG 6000) 




6%








Triton® X-100 





0.10%
0.025%




0.15%
Glycerol











50% 50% 50%
pH @ 25 7.0 7.9 7.9 7.9 7.5 7.6 8.8 7.6 7.5 6.0 9.5 8 7.4 7.4 7.4