Activity of DNA Modifying Enzymes in CutSmart Buffer

Enzyme Activity in CutSmart Buffer Required Supplements
Alkaline Phosphatase (CIP) +++  
Antarctic Phosphatase +++  Requires Zn2+
Bst DNA Polymerase +++  
CpG Methyltransferase (M. SssI) +++  
DNA Polymerase I +++  
DNA Polymerase I, Large (Klenow) Fragment
+++  
DNA Polymerase Klenow Exo- +++  
DNase I (RNase-free) +++ Requires Ca2+
E. coli DNA Ligase +++ Requires NAD
Endonuclease III (Nth), recombinant +++  
Endonuclease VIII +++  
Exonuclease III +++  
GpC Methyltransferase (M. CviPI) + Requires DTT
McrBC +++  
Micrococcal Nuclease +++  
Nuclease BAL-31 +++  
phi29 DNA Polymerase +++  
RecJf +++  
T3 DNA Ligase +++ Requires ATP + PEG
T4 DNA Ligase +++ Requires ATP
T4 DNA Polymerase +++  
T4 Phage β-glucosyltransferase (T4-BGT) +++  
T4 Polynucleotide Kinase +++ Requires ATP
T4 PNK (3 ' phosphatase minus) +++ Requires ATP
T7 DNA Ligase +++ Requires ATP + PEG
T7 Exonuclease +++  
USER Enzyme, recombinant
+++  

+++ full functional activity
++ 50-100% functional activity
+ 0-50% functional activity