Reduced Star Activities of HF® Enzymes

The following table indicates the number of units of HF®-enzyme that can be used compared to the wild type counterpart before any significant star activity is detected. The HF Factor refers to the X-fold increase that is achieved by choosing an HF enzyme. This data clearly illustrates the flexibility that is offered by using an HF restriction enzyme.

EnzymeBufferMax Units Without Star ActivityHF Factor
AgeI §NEBuffer 1.132
AgeI-HF®CutSmart® Buffer≥250≥8
ApoI §NEBuffer 3.1
EnzymeBack to topBufferMax Units Without Star ActivityHF Factor
BamHI §NEBuffer 3.132
BamHI-HF®CutSmart® Buffer≥4,000≥125
BbsI-HF®CutSmart® Buffer
BmtI §NEBuffer 3.132
BmtI-HF®CutSmart® Buffer100000062500
BsaI §CutSmart® Buffer32
BsaI-HF®CutSmart® Buffer≥8,000≥250
BsiWI-HF®CutSmart® Buffer
BsrGI §NEBuffer 2.116≥62
BsrGI-HF®CutSmart® Buffer≥2000
BstEII  §NEBuffer 3.116
BstEII-HF®CutSmart® Buffer>2000>125
BstZ17I-HF®CutSmart® Buffer
EnzymeBack to topBufferMax Units Without Star ActivityHF Factor
DraIII §NEBuffer 32
DraIII-HF®CutSmart® Buffer≥2,000≥1,000
EnzymeBack to topBufferMax Units Without Star ActivityHF Factor
EagI §NEBuffer 3.1250
EagI-HF®CutSmart® Buffer5002
EnzymeBufferMax Units Without Star ActivityHF Factor
EcoRI §NEBuffer EcoRI250
EcoRI-HF®CutSmart® Buffer16,00064
EcoRV §NEBuffer 3.11,000
EcoRV-HF®CutSmart® Buffer≥64,000≥64
EnzymeBack to topBufferMax Units Without Star ActivityHF Factor
HindIII §NEBuffer 2.1250
HindIII-HF®CutSmart® Buffer≥500,000≥2,000
EnzymeBack to topBufferMax Units Without Star ActivityHF Factor
KpnI §NEBuffer 1.116
KpnI-HF®CutSmart® Buffer≥1,000,000≥62,500
EnzymeBack to topBufferMax Units Without Star ActivityHF Factor
MfeI §CutSmart® Buffer32
MfeI-HF®CutSmart® Buffer≥500≥16
MluI §NEBuffer 3.1≥4000
MluI-HF®CutSmart® Buffer≥20002
EnzymeBack to topBufferMax Units Without Star ActivityHF Factor
NcoI §NEBuffer 3.1120
NcoI-HF®CutSmart® Buffer≥64,000≥530
NheI §NEBuffer 2.1120
NheI-HF®CutSmart® Buffer≥32,000≥266
NotI §NEBuffer 3.14,000
NotI-HF®CutSmart® Buffer≥64,000≥16
NruI §NEBuffer 3.1≥500
NruI-HF®CutSmart® Buffer≥3200064
EnzymeBufferMax Units Without Star ActivityHF Factor
NsiI §NEBuffer 3.1≥4000
NsiI-HF®CutSmart® Buffer≥80002
EnzymeBack to topBufferMax Units Without Star ActivityHF Factor
PstI §NEBuffer 3.1120
PstI-HF®CutSmart® Buffer4,00033
PvuI §NEBuffer 3.1500
PvuI-HF®CutSmart® Buffer≥16,000≥32
PvuII §NEBuffer 3.116
PvuII-HF®CutSmart® Buffer50032
EnzymeBack to topBufferMax Units Without Star ActivityHF Factor
SacI §NEBuffer 1.1120
SacI-HF®CutSmart® Buffer≥32,000≥266
SalI §NEBuffer 3.14
SalI-HF®CutSmart® Buffer≥32,000≥8,000
SbfI §CutSmart® Buffer8
SbfI-HF®CutSmart® Buffer25032
ScaI §NEBuffer 34
ScaI-HF®CutSmart® Buffer25062
SpeI §CutSmart® Buffer500
SpeI-HF®CutSmart® Buffer≥8000≥16
SphI §NEBuffer 2.132
SphI-HF®CutSmart® Buffer8,000250
EnzymeBufferMax Units Without Star ActivityHF Factor
SspI §NEBuffer SspI32
SspI-HF®CutSmart® Buffer50016
StyI §NEBuffer 3.132
StyI-HF®CutSmart® Buffer4,000125

§ A HF version of this enzyme is available.
† Wild type enzymes were tested in supplied buffer for comparisons.
* Wei, H. et al. (2008) Nucleic Acids Res. 36, e50.

HF® is a registered trademark of New England Biolabs, Inc.