LOCUS LITMUS28i-Mal 3593 bp DNA circular 11-NOV-2009 DEFINITION Control vector LITMUS28i-Mal, complete sequence. ACCESSION VERSION KEYWORDS . SOURCE Control vector LITMUS28i-Mal ORGANISM Control vector LITMUS28i-Mal other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 3593) AUTHORS Noren,K. and Noren,C.J. TITLE Direct Submission JOURNAL Submitted (11-NOV-2009) Research Department, New England Biolabs, 240 County Road, Ipswich, MA 01938, USA FEATURES Location/Qualifiers source 1..3593 /organism="Control vector LITMUS28i-Mal" /mol_type="other DNA" gene 143..1003 /gene="bla" CDS 143..1003 /gene="bla" /note="ampR (confers resistance to ampicillin)" /codon_start=1 /product="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERSPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" sig_peptide 143..211 /gene="bla" /note="Required for secretion to the periplasm; cleaved off to form the mature beta-lactamase protein." rep_origin complement(1045..1554) /note="M13 origin of replication (+ -)" rep_origin 1665..2253 /note="pMB1 origin of replication (clockwise) (RNAII -35 to RNA/DNA switch point)" promoter 2350..2379 /note="Plac promoter (-35 signal TTTACA, -10 signal TATGTT)" promoter 2449..2466 /note="T7 promoter (transcript start 2466 clockwise)" misc_feature 2470..3283 /note="BglII-EcoRI fragment of malE (from pMAL-p2X); template for transcription from T7 promoter." misc_feature 3369..3374 /note="StuI restriction site; as supplied in the HiScribe T7 In Vitro Transcription Kit (NEB #E2030S), LITMUS28i-Mal is linearized at this site" promoter complement(3373..3390) /note="T7 promoter (transcript start 3373 counter-clockwise)" BASE COUNT 973 a 869 c 916 g 835 t ORIGIN 1 gttaactacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt 61 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca 121 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt 181 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga 241 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa 301 gatccttgag agttttcgcc ccgaagaacg ttctccaatg atgagcactt ttaaagttct 361 gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat 421 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga 481 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc 541 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat 601 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa 661 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac 721 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa 781 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc 841 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc 901 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag 961 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta 1021 ctcatatata ctttagattg atttaccccg gttgataatc agaaaagccc caaaaacagg 1081 aagattgtat aagcaaatat ttaaattgta aacgttaata ttttgttaaa attcgcgtta 1141 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat 1201 aaatcaaaag aatagcccga gatagggttg agtgttgttc cagtttggaa caagagtcca 1261 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc 1321 ccactacgtg aaccatcacc caaatcaagt tttttggggt cgaggtgccg taaagcacta 1381 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcg aacgtggcga 1441 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca 1501 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtaaaagg 1561 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg 1621 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt 1681 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg 1741 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata 1801 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca 1861 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag 1921 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc 1981 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga 2041 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg 2101 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac 2161 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg 2221 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg 2281 ttcctggcct tttgctggcc ttttgctcac atgtaatgtg agttagctca ctcattaggc 2341 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata 2401 acaatttcac acaggaaaca gctatgacca tgattacgcc aagctacgta atacgactca 2461 ctatagggca gatctgctgc cgaacccgcc aaaaacctgg gaagagatcc cggcgctgga 2521 taaagaactg aaagcgaaag gtaagagcgc gctgatgttc aacctgcaag aaccgtactt 2581 cacctggccg ctgattgctg ctgacggggg ttatgcgttc aagtatgaaa acggcaagta 2641 cgacattaaa gacgtgggcg tggataacgc tggcgcgaaa gcgggtctga ccttcctggt 2701 tgacctgatt aaaaacaaac acatgaatgc agacaccgat tactccatcg cagaagctgc 2761 ctttaataaa ggcgaaacag cgatgaccat caacggcccg tgggcatggt ccaacatcga 2821 caccagcaaa gtgaattatg gtgtaacggt actgccgacc ttcaagggtc aaccatccaa 2881 accgttcgtt ggcgtgctga gcgcaggtat taacgccgcc agtccgaaca aagagctggc 2941 aaaagagttc ctcgaaaact atctgctgac tgatgaaggt ctggaagcgg ttaataaaga 3001 caaaccgctg ggtgccgtag cgctgaagtc ttacgaggaa gagttggcga aagatccacg 3061 tattgccgcc actatggaaa acgcccagaa aggtgaaatc atgccgaaca tcccgcagat 3121 gtccgctttc tggtatgccg tgcgtactgc ggtgatcaac gccgccagcg gtcgtcagac 3181 tgtcgatgaa gccctgaaag acgcgcagac taattcgagc tcgaacaaca acaacaataa 3241 caataacaac aacctcggga tcgagggaag gatttcagaa ttcctgcagg atatctggat 3301 ccacgaagct tcccatggtg acgtcaccgg ttctagatac ctaggtgagc tctggtaccc 3361 tctagtcaag gcctatagtg agtcgtatta cggactggcc gtcgttttac aacgtcgtga 3421 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag 3481 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa 3541 tggcgaatgg cgcttcgctt ggtaataaag cccgcttcgg cgggcttttt ttt //