LOCUS pBeloBAC11 7507 bp DNA circular 19-OCT-2007 DEFINITION Cloning vector pBeloBAC11, complete sequence. ACCESSION VERSION KEYWORDS . SOURCE Cloning vector pBeloBAC11 ORGANISM Cloning vector pBeloBAC11 other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 7507) AUTHORS New England Biolabs. TITLE Direct Submission JOURNAL Submitted (19-OCT-2007) Research Department, New England Biolabs, 240 County Road, Ipswich, MA 01938, USA COMMENT See also GenBank accession U51113. FEATURES Location/Qualifiers source 1..7507 /organism="Cloning vector pBeloBAC11" /mol_type="other DNA" gene complement(56..436) /gene="lacZalpha" CDS complement(56..436) /gene="lacZalpha" /codon_start=1 /product="beta-galactosidase alpha fragment" /translation="MTMITPSYLGDTIEYSSLHACRSTLEDPRVPSSNSPYSESYYNS LAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYF LLTHLCGISHRIWCTLSTICSDAA" promoter 312..329 /note="T7 promoter (transcript start 329 clockwise)" promoter complement(398..414) /gene="lacZalpha" /note="SP6 promoter (transcript counterclockwise)" gene complement(766..1425) /gene="cat" CDS complement(766..1425) /gene="cat" /note="camR (confers resistance to chloramphenicol)" /codon_start=1 /product="chloramphenicol acetyltransferase" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG A" rep_origin 2370..2436 /note="ori2 (oriS)" gene 2765..3520 /gene="repE" CDS 2765..3520 /gene="repE" /codon_start=1 /product="replication initiation protein E" /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVD QIRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEED AGDEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNP YAMRLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINS RTPMRLSYIEKKKGRQTTHIVFSFRDITSMTTG" gene 4108..5274 /gene="sopA" CDS 4108..5274 /gene="sopA" /note="stability of plasmid" /codon_start=1 /product="plasmid partition protein A" /translation="MKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRIGE AADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRAE DVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPT DPHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQ FFDMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRE TDEVGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR" gene 5274..6245 /gene="sopB" CDS 5274..6245 /gene="sopB" /note="stability of plasmid" /codon_start=1 /product="plasmid partition protein B" /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGN AITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQ TPAFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSA YERGQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSA RSGDALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLS SRHQFAPGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP" misc_feature 6318..6791 /note="sopC (repeat region bound by SopB)" misc_feature 7050..7449 /note="lambda cos site" protein_bind 7467..7500 /note="loxP site" /bound_moiety="Cre recombinase" BASE COUNT 1865 a 1689 c 1832 g 2121 t ORIGIN 1 gcggccgcaa ggggttcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg 61 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat 121 gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg 181 aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg 241 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg 301 ccagtgaatt gtaatacgac tcactatagg gcgaattcga gctcggtacc cggggatcct 361 ctagagtcga cctgcaggca tgcaagcttg agtattctat agtgtcacct aaatagcttg 421 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac 481 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc 541 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg 601 cattaatgaa tcggccaacg cgaacccctt gcggccgccc gggccgtcga ccaattctca 661 tgtttgacag cttatcatcg aatttctgcc attcatccgc ttattatcac ttattcaggc 721 gtagcaacca ggcgtttaag ggcaccaata actgccttaa aaaaattacg ccccgccctg 781 ccactcatcg cagtactgtt gtaattcatt aagcattctg ccgacatgga agccatcaca 841 aacggcatga tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt gcgtataata 901 tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa 961 actggtgaaa ctcacccagg gattggctga gacgaaaaac atattctcaa taaacccttt 1021 agggaaatag gccaggtttt caccgtaaca cgccacatct tgcgaatata tgtgtagaaa 1081 ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa aacgtttcag tttgctcatg 1141 gaaaacggtg taacaagggt gaacactatc ccatatcacc agctcaccgt ctttcattgc 1201 catacggaat tccggatgag cattcatcag gcgggcaaga atgtgaataa aggccggata 1261 aaacttgtgc ttatttttct ttacggtctt taaaaaggcc gtaatatcca gctgaacggt 1321 ctggttatag gtacattgag caactgactg aaatgcctca aaatgttctt tacgatgcca 1381 ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc 1441 tcctgaaaat ctcgataact caaaaaatac gcccggtagt gatcttattt cattatggtg 1501 aaagttggaa cctcttacgt gccgatcaac gtctcatttt cgccaaaagt tggcccaggg 1561 cttcccggta tcaacaggga caccaggatt tatttattct gcgaagtgat cttccgtcac 1621 aggtatttat tcgcgataag ctcatggagc ggcgtaaccg tcgcacagga aggacagaga 1681 aagcgcggat ctgggaagtg acggacagaa cggtcaggac ctggattggg gaggcggttg 1741 ccgccgctgc tgctgacggt gtgacgttct ctgttccggt cacaccacat acgttccgcc 1801 attcctatgc gatgcacatg ctgtatgccg gtataccgct gaaagttctg caaagcctga 1861 tgggacataa gtccatcagt tcaacggaag tctacacgaa ggtttttgcg ctggatgtgg 1921 ctgcccggca ccgggtgcag tttgcgatgc cggagtctga tgcggttgcg atgctgaaac 1981 aattatcctg agaataaatg ccttggcctt tatatggaaa tgtggaactg agtggatatg 2041 ctgtttttgt ctgttaaaca gagaagctgg ctgttatcca ctgagaagcg aacgaaacag 2101 tcgggaaaat ctcccattat cgtagagatc cgcattatta atctcaggag cctgtgtagc 2161 gtttatagga agtagtgttc tgtcatgatg cctgcaagcg gtaacgaaaa cgatttgaat 2221 atgccttcag gaacaataga aatcttcgtg cggtgttacg ttgaagtgga gcggattatg 2281 tcagcaatgg acagaacaac ctaatgaaca cagaaccatg atgtggtctg tccttttaca 2341 gccagtagtg ctcgccgcag tcgagcgaca gggcgaagcc ctcgagtgag cgaggaagca 2401 ccagggaaca gcacttatat attctgctta cacacgatgc ctgaaaaaac ttcccttggg 2461 gttatccact tatccacggg gatattttta taattatttt ttttatagtt tttagatctt 2521 cttttttaga gcgccttgta ggcctttatc catgctggtt ctagagaagg tgttgtgaca 2581 aattgccctt tcagtgtgac aaatcaccct caaatgacag tcctgtctgt gacaaattgc 2641 ccttaaccct gtgacaaatt gccctcagaa gaagctgttt tttcacaaag ttatccctgc 2701 ttattgactc ttttttattt agtgtgacaa tctaaaaact tgtcacactt cacatggatc 2761 tgtcatggcg gaaacagcgg ttatcaatca caagaaacgt aaaaatagcc cgcgaatcgt 2821 ccagtcaaac gacctcactg aggcggcata tagtctctcc cgggatcaaa aacgtatgct 2881 gtatctgttc gttgaccaga tcagaaaatc tgatggcacc ctacaggaac atgacggtat 2941 ctgcgagatc catgttgcta aatatgctga aatattcgga ttgacctctg cggaagccag 3001 taaggatata cggcaggcat tgaagagttt cgcggggaag gaagtggttt tttatcgccc 3061 tgaagaggat gccggcgatg aaaaaggcta tgaatctttt ccttggttta tcaaacgtgc 3121 gcacagtcca tccagagggc tttacagtgt acatatcaac ccatatctca ttcccttctt 3181 tatcgggtta cagaaccggt ttacgcagtt tcggcttagt gaaacaaaag aaatcaccaa 3241 tccgtatgcc atgcgtttat acgaatccct gtgtcagtat cgtaagccgg atggctcagg 3301 catcgtctct ctgaaaatcg actggatcat agagcgttac cagctgcctc aaagttacca 3361 gcgtatgcct gacttccgcc gccgcttcct gcaggtctgt gttaatgaga tcaacagcag 3421 aactccaatg cgcctctcat acattgagaa aaagaaaggc cgccagacga ctcatatcgt 3481 attttccttc cgcgatatca cttccatgac gacaggatag tctgagggtt atctgtcaca 3541 gatttgaggg tggttcgtca catttgttct gacctactga gggtaatttg tcacagtttt 3601 gctgtttcct tcagcctgca tggattttct catacttttt gaactgtaat ttttaaggaa 3661 gccaaatttg agggcagttt gtcacagttg atttccttct ctttcccttc gtcatgtgac 3721 ctgatatcgg gggttagttc gtcatcattg atgagggttg attatcacag tttattactc 3781 tgaattggct atccgcgtgt gtacctctac ctggagtttt tcccacggtg gatatttctt 3841 cttgcgctga gcgtaagagc tatctgacag aacagttctt ctttgcttcc tcgccagttc 3901 gctcgctatg ctcggttaca cggctgcggc gagcgctagt gataataagt gactgaggta 3961 tgtgctcttc ttatctcctt ttgtagtgtt gctcttattt taaacaactt tgcggttttt 4021 tgatgacttt gcgattttgt tgttgctttg cagtaaattg caagatttaa taaaaaaacg 4081 caaagcaatg attaaaggat gttcagaatg aaactcatgg aaacacttaa ccagtgcata 4141 aacgctggtc atgaaatgac gaaggctatc gccattgcac agtttaatga tgacagcccg 4201 gaagcgagga aaataacccg gcgctggaga ataggtgaag cagcggattt agttggggtt 4261 tcttctcagg ctatcagaga tgccgagaaa gcagggcgac taccgcaccc ggatatggaa 4321 attcgaggac gggttgagca acgtgttggt tatacaattg aacaaattaa tcatatgcgt 4381 gatgtgtttg gtacgcgatt gcgacgtgct gaagacgtat ttccaccggt gatcggggtt 4441 gctgcccata aaggtggcgt ttacaaaacc tcagtttctg ttcatcttgc tcaggatctg 4501 gctctgaagg ggctacgtgt tttgctcgtg gaaggtaacg acccccaggg aacagcctca 4561 atgtatcacg gatgggtacc agatcttcat attcatgcag aagacactct cctgcctttc 4621 tatcttgggg aaaaggacga tgtcacttat gcaataaagc ccacttgctg gccggggctt 4681 gacattattc cttcctgtct ggctctgcac cgtattgaaa ctgagttaat gggcaaattt 4741 gatgaaggta aactgcccac cgatccacac ctgatgctcc gactggccat tgaaactgtt 4801 gctcatgact atgatgtcat agttattgac agcgcgccta acctgggtat cggcacgatt 4861 aatgtcgtat gtgctgctga tgtgctgatt gttcccacgc ctgctgagtt gtttgactac 4921 acctccgcac tgcagttttt cgatatgctt cgtgatctgc tcaagaacgt tgatcttaaa 4981 gggttcgagc ctgatgtacg tattttgctt accaaataca gcaatagtaa tggctctcag 5041 tccccgtgga tggaggagca aattcgggat gcctggggaa gcatggttct aaaaaatgtt 5101 gtacgtgaaa cggatgaagt tggtaaaggt cagatccgga tgagaactgt ttttgaacag 5161 gccattgatc aacgctcttc aactggtgcc tggagaaatg ctctttctat ttgggaacct 5221 gtctgcaatg aaattttcga tcgtctgatt aaaccacgct gggagattag ataatgaagc 5281 gtgcgcctgt tattccaaaa catacgctca atactcaacc ggttgaagat acttcgttat 5341 cgacaccagc tgccccgatg gtggattcgt taattgcgcg cgtaggagta atggctcgcg 5401 gtaatgccat tactttgcct gtatgtggtc gggatgtgaa gtttactctt gaagtgctcc 5461 ggggtgatag tgttgagaag acctctcggg tatggtcagg taatgaacgt gaccaggagc 5521 tgcttactga ggacgcactg gatgatctca tcccttcttt tctactgact ggtcaacaga 5581 caccggcgtt cggtcgaaga gtatctggtg tcatagaaat tgccgatggg agtcgccgtc 5641 gtaaagctgc tgcacttacc gaaagtgatt atcgtgttct ggttggcgag ctggatgatg 5701 agcagatggc tgcattatcc agattgggta acgattatcg cccaacaagt gcttatgaac 5761 gtggtcagcg ttatgcaagc cgattgcaga atgaatttgc tggaaatatt tctgcgctgg 5821 ctgatgcgga aaatatttca cgtaagatta ttacccgctg tatcaacacc gccaaattgc 5881 ctaaatcagt tgttgctctt ttttctcacc ccggtgaact atctgcccgg tcaggtgatg 5941 cacttcaaaa agcctttaca gataaagagg aattacttaa gcagcaggca tctaaccttc 6001 atgagcagaa aaaagctggg gtgatatttg aagctgaaga agttatcact cttttaactt 6061 ctgtgcttaa aacgtcatct gcatcaagaa ctagtttaag ctcacgacat cagtttgctc 6121 ctggagcgac agtattgtat aagggcgata aaatggtgct taacctggac aggtctcgtg 6181 ttccaactga gtgtatagag aaaattgagg ccattcttaa ggaacttgaa aagccagcac 6241 cctgatgcga ccacgtttta gtctacgttt atctgtcttt acttaatgtc ctttgttaca 6301 ggccagaaag cataactggc ctgaatattc tctctgggcc cactgttcca cttgtatcgt 6361 cggtctgata atcagactgg gaccacggtc ccactcgtat cgtcggtctg attattagtc 6421 tgggaccacg gtcccactcg tatcgtcggt ctgattatta gtctgggacc acggtcccac 6481 tcgtatcgtc ggtctgataa tcagactggg accacggtcc cactcgtatc gtcggtctga 6541 ttattagtct gggaccatgg tcccactcgt atcgtcggtc tgattattag tctgggacca 6601 cggtcccact cgtatcgtcg gtctgattat tagtctggaa ccacggtccc actcgtatcg 6661 tcggtctgat tattagtctg ggaccacggt cccactcgta tcgtcggtct gattattagt 6721 ctgggaccac gatcccactc gtgttgtcgg tctgattatc ggtctgggac cacggtccca 6781 cttgtattgt cgatcagact atcagcgtga gactacgatt ccatcaatgc ctgtcaaggg 6841 caagtattga catgtcgtcg taacctgtag aacggagtaa cctcggtgtg cggttgtatg 6901 cctgctgtgg attgctgctg tgtcctgctt atccacaaca ttttgcgcac ggttatgtgg 6961 acaaaatacc tggttaccca ggccgtgccg gcacgttaac cgggctgcat ccgatgcaag 7021 tgtgtcgctg tcgacgagct cgcgagctcg gacatgaggt tgccccgtat tcagtgtcgc 7081 tgatttgtat tgtctgaagt tgtttttacg ttaagttgat gcagatcaat taatacgata 7141 cctgcgtcat aattgattat ttgacgtggt ttgatggcct ccacgcacgt tgtgatatgt 7201 agatgataat cattatcact ttacgggtcc tttccggtga tccgacaggt tacggggcgg 7261 cgacctcgcg ggttttcgct atttatgaaa attttccggt ttaaggcgtt tccgttcttc 7321 ttcgtcataa cttaatgttt ttatttaaaa taccctctga aaagaaagga aacgacaggt 7381 gctgaaagcg agctttttgg cctctgtcgt ttcctttctc tgtttttgtc cgtggaatga 7441 acaatggaag tccgagctca tcgctaataa cttcgtatag catacattat acgaagttat 7501 attcgat //