LOCUS pFOS1 9738 bp DNA circular 19-OCT-2007 DEFINITION Cloning vector pFOS1, complete sequence. ACCESSION VERSION KEYWORDS . SOURCE Cloning vector pFOS1 ORGANISM Cloning vector pFOS1 other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 9738) AUTHORS New England Biolabs. TITLE Direct Submission JOURNAL Submitted (19-OCT-2007) Research Department, New England Biolabs, 240 County Road, Ipswich, MA 01938, USA FEATURES Location/Qualifiers source 1..9738 /organism="Cloning vector pFOS1" /mol_type="other DNA" gene 134..994 /gene="bla" CDS 134..994 /gene="bla" /note="ampR (confers resistance to ampicillin)" /codon_start=1 /product="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" sig_peptide 134..202 /gene="bla" /note="required for secretion to the periplasm; cleaved off to form the mature beta-lactamase protein" rep_origin 1165..1753 /note="pUC19 origin of replication (clockwise) (RNAII -35 to RNA/DNA switch point)" misc_feature 2024..2422 /note="lambda cos site" protein_bind 2440..2473 /note="loxP site" /bound_moiety="Cre recombinase" promoter 2489..2506 /note="T7 promoter (transcript start 2506 clockwise)" promoter complement(2523..2539) /note="SP6 promoter (transcript counterclockwise)" gene complement(2679..3338) /gene="cat" CDS complement(2679..3338) /gene="cat" /note="camR (confers resistance to chloramphenicol)" /codon_start=1 /product="chloramphenicol acetyltransferase" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG A" rep_origin 4280..4346 /note="ori2 (oriS)" gene 4675..5430 /gene="repE" CDS 4675..5430 /gene="repE" /codon_start=1 /product="replication initiation protein E" /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVD QIRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEED AGDEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNP YAMRLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINS RTPMRLSYIEKKKGRQTTHIVFSFRDITSMTTG" gene 6009..7184 /gene="sopA" CDS 6009..7184 /gene="sopA" /note="stability of plasmid" /codon_start=1 /product="plasmid partition protein A" /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWR IGEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLR RAEDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWV PDLHIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGK LPTDPHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTS ALQFFDMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNV VRETDEVGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR" gene 7184..8155 /gene="sopB" CDS 7184..8155 /gene="sopB" /note="stability of plasmid" /codon_start=1 /product="plasmid partition protein B" /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGN AITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQ TPAFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSA YERGQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSA RSGDALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLS SRHQFAPGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP" misc_feature 8228..8701 /note="sopC (repeat region bound by SopB)" misc_feature 8960..9630 /note="lambda cos site" BASE COUNT 2409 a 2234 c 2363 g 2732 t ORIGIN 1 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat 61 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg 121 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc 181 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga 241 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga 301 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg 361 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc 421 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac 481 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact 541 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca 601 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg 661 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact 721 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg 781 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg 841 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat 901 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc 961 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat 1021 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt 1081 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc 1141 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt 1201 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac 1261 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt 1321 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct 1381 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga 1441 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac 1501 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagcattg 1561 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt 1621 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc 1681 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg 1741 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc 1801 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc 1861 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag 1921 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca 1981 ttaatgcagc ttgcgccgtt ccacttcgat gcgtcagtga agcgacatga ggttgccccg 2041 tattcagtgt cgctgatttg tattgtctga agttgttttt acgttaagtt gatgcagatc 2101 aattaatacg atacctgcgt cataattgat tatttgacgt ggtttgatgg cctccacgca 2161 cgttgtgata tgtagatgat aatcattatc actttacggg tcctttccgg tgatccgaca 2221 ggttacgggg cggcgacctc gcgggttttc gctatttatg aaaattttcc ggtttaaggc 2281 gtttccgttc ttcttcgtca taacttaatg tttttattta aaataccctc tgaaaagaaa 2341 ggaaacgaca ggtgctgaaa gcgagctttt tggcctctgt cgtttccttt ctctgttttt 2401 gtccgtggaa tgaacaatgg aagtccgagc tcatcgctaa taacttcgta tagcatacat 2461 tatacgaagt tatattcgat gcggccgcta atacgactca ctatagggag aagcttggat 2521 cctatagtgt cacctaaatc gtatgcggcc gcccgggccg tcgaccaatt ctcatgtttg 2581 acagcttatc atcgaatttc tgccattcat ccgcttatta tcacttattc aggcgtagca 2641 ccaggcgttt aagggcacca ataactgcct taaaaaaatt acgccccgcc ctgccactca 2701 tcgcagtact gttgtaattc attaagcatt ctgccgacat ggaagccatc acagacggca 2761 tgatgaacct gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc 2821 atggtgaaaa cgggggcgaa gaagttgtcc atattggcca cgtttaaatc aaaactggtg 2881 aaactcaccc agggattggc tgagacgaaa aacatattct caataaaccc tttagggaaa 2941 taggccaggt tttcaccgta acacgccaca tcttgcgaat atatgtgtag aaactgccgg 3001 aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt cagtttgctc atggaaaacg 3061 gtgtaacaag ggtgaacact atcccatatc accagctcac cgtctttcat tgccatacgg 3121 aattccggat gagcattcat caggcgggca agaatgtgaa taaaggccgg ataaaacttg 3181 tgcttatttt tctttacggt ctttaaaaag gccgtaatat ccagctgaac ggtctggtta 3241 taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat 3301 atatcaacgg tggtatatcc agtgattttt ttctccattt tagcttcctt agctcctgaa 3361 aatctcgata actcaaaaaa tacgcccggt agtgatctta tttcattatg gtgaaagttg 3421 gaacctctta cgtgccgatc aacgtctcat tttcgccaaa agttggccca gggcttcccg 3481 gtatcaacag ggacaccagg atttatttat tctgcgaagt gatcttccgt cacaggtatt 3541 tattcgcgat aagctcatgg agcggcgtaa ccgtcgcaca ggaaggacag agaaagcgcg 3601 gatctgggaa gtgacggaca gaacggtcag gacctggatt ggggaggcgg ttgccgccgc 3661 tgctgctgac ggtgtgacgt tctctgttcc ggtcacacca catacgttcc gccattccta 3721 tgcgatgcac atgctgtatg ccggtatacc gctgaaagtt ctgcaaagcc tgatgggaca 3781 taagtccatc agttcaacgg aagtctacac gaaggttttt gcgctggatg tggctgcccg 3841 gcaccgggtg cagtttgcga tgccggagtc tgatgcggtt gcgatgctga aacaattatc 3901 ctgagaataa atgccttggc ctttatatgg aaatgtggaa ctgagtggat atgctgtttt 3961 tgtctgttaa acagagaagc tggctgttat ccactgagaa gcgaacgaaa cagtcgggaa 4021 aatctcccat tatcgtagag atccgcatta ttaatctcag gagcctgtgt agcgtttata 4081 ggaagtagtg ttctgtcatg atgcctgcaa gcggtaacga aaacgatttg aatatgcctt 4141 caggaacaat agaaatcttc gtgcggtgtt acgttgaagt ggagcggatt atgtcagcaa 4201 tggacagaac aacctaatga acacagaacc atgatgtggt ctgtcctttt acagccagta 4261 ggctcgccgc agtcgagcga cggcgaagcc ctcgagtgag cgaggaagca ccagggaaca 4321 gcacttatat attctgctta cacacgatgc ctgaaaaaac ttcccttggg gttatccact 4381 tatccacggg gatattttta taattatttt ttttatagtt tttagatctt cttttttaga 4441 gcgccttgta ggcctttatc catgctggtt ctagagaagg tgttgtgaca aattgccctt 4501 tcagtgtgac aaatcaccct caaatgacag tcctgtctgt gacaaattgc ccttaaccct 4561 gtgacaaatt gccctcagaa gaagctgttt tttcacaaag ttatccctgc ttattgactc 4621 ttttttattt agtgtgacaa tctaaaaact tgtcacactt cacatggatc tgtcatggcg 4681 gaaacagcgg ttatcaatca caagaaacgt aaaaatagcc cgcgaatcgt ccagtcaaac 4741 gacctcactg aggcggcata tagtctctcc cgggatcaaa aacgtatgct gtatctgttc 4801 gttgaccaga tcagaaaatc tgatggcacc ctacaggaac atgacggtat ctgcgagatc 4861 catgttgcta aatatgctga aatattcgga ttgacctctg cggaagccag taaggatata 4921 cggcaggcat tgaagagttt cgcggggaag gaagtggttt tttatcgccc tgaagaggat 4981 gccggcgatg aaaaaggcta tgaatctttt ccttggttta tcaaacgtgc gcacagtcca 5041 tccagagggc tttacagtgt acatatcaac ccatatctca ttcccttctt tatcgggtta 5101 cagaaccggt ttacgcagtt tcggcttagt gaaacaaaag aaatcaccaa tccgtatgcc 5161 atgcgtttat acgaatccct gtgtcagtat cgtaagccgg atggctcagg catcgtctct 5221 ctgaaaatcg actggatcat agagcgttac cagctgcctc aaagttacca gcgtatgcct 5281 gacttccgcc gccgcttcct gcaggtctgt gttaatgaga tcaacagcag aactccaatg 5341 cgcctctcat acattgagaa aaagaaaggc cgccagacga ctcatatcgt attttccttc 5401 cgcgatatca cttccatgac gacaggatag tctgagggtt atctgtcaca gatttgaggg 5461 tggttcgtca catttgttct gacctactga gggtaatttg tcacagtttt gctgtttcct 5521 tcagcctgca tggattttct catacttttt gaactgtaat ttttaaggaa gccaaatttg 5581 agggcagttt gtcacagttg atttccttct ctttcccttc gtcatgtgac ctgatatcgg 5641 gggttagttc gtcatcattg atgagggttg attatcacag tttattactc tgaattggct 5701 atccgcgtgt gtacctctac ctggagtttt tcccacggtg gatatttctt cttgcgctga 5761 gcgtaagagc tatctgacag aacagttctt ctttgcttcc tcgccagttc gctcgctatg 5821 ctcggttaca cggctgcggc gagcgctagt gataataagt gactgaggta tgtgctcttc 5881 ttatctcctt ttgtagtgtt gctcttattt taaacaactt tgcggttttt tgatgacttt 5941 gcgattttgt tgttgctttg cagtaaattg caagatttaa taaaaaaacg caaagcaatg 6001 attaaaggat gttcagaatg aaactcatgg aaacacttaa ccagtgcata aacgctggtc 6061 atgaaatgac gaaggctatc gccattgcac agtttaatga tgacagcccg gaagcgagga 6121 aaataacccg gcgctggaga ataggtgaag cagcggattt agttggggtt tcttctcagg 6181 ctatcagaga tgccgagaaa gcagggcgac taccgcaccc ggatatggaa attcgaggac 6241 gggttgagca acgtgttggt tatacaattg aacaaattaa tcatatgcgt gatgtgtttg 6301 gtacgcgatt gcgacgtgct gaagacgtat ttccaccggt gatcggggtt gctgcccata 6361 aaggtggcgt ttacaaaacc tcagtttctg ttcatcttgc tcaggatctg gctctgaagg 6421 ggctacgtgt tttgctcgtg gaaggtaacg acccccaggg aacagcctca atgtatcacg 6481 gatgggtacc agatcttcat attcatgcag aagacactct cctgcctttc tatcttgggg 6541 aaaaggacga tgtcacttat gcaataaagc ccacttgctg gccggggctt gacattattc 6601 cttcctgtct ggctctgcac cgtattgaaa ctgagttaat gggcaaattt gatgaaggta 6661 aactgcccac cgatccacac ctgatgctcc gactggccat tgaaactgtt gctcatgact 6721 atgatgtcat agttattgac agcgcgccta acctgggtat cggcacgatt aatgtcgtat 6781 gtgctgctga tgtgctgatt gttcccacgc ctgctgagtt gtttgactac acctccgcac 6841 tgcagttttt cgatatgctt cgtgatctgc tcaagaacgt tgatcttaaa gggttcgagc 6901 ctgatgtacg tattttgctt accaaataca gcaatagtaa tggctctcag tccccgtgga 6961 tggaggagca aattcgggat gcctggggaa gcatggttct aaaaaatgtt gtacgtgaaa 7021 cggatgaagt tggtaaaggt cagatccgga tgagaactgt ttttgaacag gccattgatc 7081 aacgctcttc aactggtgcc tggagaaatg ctctttctat ttgggaacct gtctgcaatg 7141 aaattttcga tcgtctgatt aaaccacgct gggagattag ataatgaagc gtgcgcctgt 7201 tattccaaaa catacgctca atactcaacc ggttgaagat acttcgttat cgacaccagc 7261 tgccccgatg gtggattcgt taattgcgcg cgtaggagta atggctcgcg gtaatgccat 7321 tactttgcct gtatgtggtc gggatgtgaa gtttactctt gaagtgctcc ggggtgatag 7381 tgttgagaag acctctcggg tatggtcagg taatgaacgt gaccaggagc tgcttactga 7441 ggacgcactg gatgatctca tcccttcttt tctactgact ggtcaacaga caccggcgtt 7501 cggtcgaaga gtatctggtg tcatagaaat tgccgatggg agtcgccgtc gtaaagctgc 7561 tgcacttacc gaaagtgatt atcgtgttct ggttggcgag ctggatgatg agcagatggc 7621 tgcattatcc agattgggta acgattatcg cccaacaagt gcttatgaac gtggtcagcg 7681 ttatgcaagc cgattgcaga atgaatttgc tggaaatatt tctgcgctgg ctgatgcgga 7741 aaatatttca cgtaagatta ttacccgctg tatcaacacc gccaaattgc ctaaatcagt 7801 tgttgctctt ttttctcacc ccggtgaact atctgcccgg tcaggtgatg cacttcaaaa 7861 agcctttaca gataaagagg aattacttaa gcagcaggca tctaaccttc atgagcagaa 7921 aaaagctggg gtgatatttg aagctgaaga agttatcact cttttaactt ctgtgcttaa 7981 aacgtcatct gcatcaagaa ctagtttaag ctcacgacat cagtttgctc ctggagcgac 8041 agtattgtat aagggcgata aaatggtgct taacctggac aggtctcgtg ttccaactga 8101 gtgtatagag aaaattgagg ccattcttaa ggaacttgaa aagccagcac cctgatgcga 8161 ccacgtttta gtctacgttt atctgtcttt acttaatgtc ctttgttaca ggccagaaag 8221 cataactggc ctgaatattc tctctgggcc cactgttcca cttgtatcgt cggtctgata 8281 atcagactgg gaccacggtc ccactcgtat cgtcggtctg attattagtc tgggaccacg 8341 gtcccactcg tatcgtcggt ctgattatta gtctgggacc acggtcccac tcgtatcgtc 8401 ggtctgataa tcagactggg accacggtcc cactcgtatc gtcggtctga ttattagtct 8461 gggaccatgg tcccactcgt atcgtcggtc tgattattag tctgggacca cggtcccact 8521 cgtatcgtcg gtctgattat tagtctggaa ccacggtccc actcgtatcg tcggtctgat 8581 tattagtctg ggaccacggt cccactcgta tcgtcggtct gattattagt ctgggaccac 8641 gatcccactc gtgttgtcgg tctgattatc ggtctgggac cacggtccca cttgtattgt 8701 cgatcagact atcagcgtga gactacgatt ccatcaatgc ctgtcaaggg caagtattga 8761 catgtcgtcg taacctgtag aacggagtaa cctcggtgtg cggttgtatg cctgctgtgg 8821 attgctgctg tgtcctgctt atccacaaca ttttgcgcac ggttatgtgg acaaaatacc 8881 tggttaccca ggccgtgccg gcacgttaac cgggctgcat ccgatgcaag tgtgtcgctg 8941 tcgacgagct cgcgagctcg gacatgaggt tgccccgtat tcagtgtcgc tgatttgtat 9001 tgtctgaagt tgtttttacg ttaagttgat gcagatcaat taatacgata cctgcgtcat 9061 aattgattat ttgacgtggt ttgatggcct ccacgcacgt tgtgatatgt agatgataat 9121 cattatcact ttacgggtcc tttccggtga tccgacaggt tacggggcgg cgacctcgcg 9181 ggttttcgct atttatgaaa attttccggt ttaaggcgtt tccgttcttc ttcgtcataa 9241 cttaatgttt ttatttaaaa taccctctga aaagaaagga aacgacaggt gctgaaagcg 9301 aggctttttg gcctctgtcg tttcctttct ctgtttttgt ccgtggaatg aacaatggaa 9361 gtcctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct 9421 gaatggcgaa tggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca 9481 ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg 9541 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta 9601 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc 9661 gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat 9721 aataatggtt tcttagac //