LOCUS pGLuc-Basic_2 4958 bp DNA circular 22-DEC-2016 DEFINITION Cloning vector pGLuc-Basic_2, complete sequence. ACCESSION VERSION KEYWORDS . SOURCE Cloning vector pGLuc-Basic_2 ORGANISM Cloning vector pGLuc-Basic_2 Unclassified. REFERENCE 1 (bases 1 to 4958) AUTHORS Tzertzinis,G. TITLE Direct Submission JOURNAL Submitted (24-SEP-2012) Research Department, New England Biolabs, 240 County Road, Ipswich, MA 01938, USA FEATURES Location/Qualifiers source 1..4958 /organism="Cloning vector pGLuc-Basic_2" /mol_type="other DNA" misc_feature 12..68 /note="multiple cloning site (BglII-BamHI)" gene 76..633 /gene="GLuc" CDS 76..633 /gene="GLuc" /codon_start=1 /product="Gaussia princeps luciferase" /translation="MGVKVLFALICIAVAEAKPTENNEDFNIVAVASNFATTDLDADR GKLPGKKLPLEVLKEMEANARKAGCTRGCLICLSHIKCTPKMKKFIPGRCHTYEGDKE SAQGGIGEAIVDIPEIPGFKDLEPMEQFIAQVDLCVDCTTGCLKGLANVQCSDLLKKW LPQRCATFASKIQGQVDKIKGAGGD" polyA_signal 642..690 /note="synthetic polyA" promoter 1276..1611 /note="SV40 promoter (clockwise)" gene 1663..2457 /gene="aph(3')-II" CDS 1663..2457 /gene="aph(3')-II" /note="neoR, kanR, nptII (confers resistance to neomycin and kanamycin)" /codon_start=1 /product="aminoglycoside phosphotransferase from Tn5" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" polyA_signal 2631..2761 /gene="aph(3')-II" /note="SV40 polyA signal sequence" promoter complement(2850..2879) /note="Plac promoter (-35 signal TTTACA, -10 signal TATGTT)" rep_origin complement(3203..3791) /note="pUC19 origin of replication (counter-clockwise) (RNAII -35 to RNA/DNA switch point)" gene complement(3962..4822) /gene="bla" CDS complement(3962..4822) /gene="bla" /note="ampR (confers resistance to ampicillin)" /codon_start=1 /product="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" BASE COUNT 1136 a 1306 c 1293 g 1223 t ORIGIN 1 gacggatcgg gagatcttgg aattctgcag atatcctcga gcccaagctt ggtaccgagc 61 tcggatccag ccaccatggg agtcaaagtt ctgtttgccc tgatctgcat cgctgtggcc 121 gaggccaagc ccaccgagaa caacgaagac ttcaacatcg tggccgtggc cagcaacttc 181 gcgaccacgg atctcgatgc tgaccgcggg aagttgcccg gcaagaagct gccgctggag 241 gtgctcaaag agatggaagc caatgcccgg aaagctggct gcaccagggg ctgtctgatc 301 tgcctgtccc acatcaagtg cacgcccaag atgaagaagt tcatcccagg acgctgccac 361 acctacgaag gcgacaaaga gtccgcacag ggcggcatag gcgaggcgat cgtcgacatt 421 cctgagattc ctgggttcaa ggacttggag cccatggagc agttcatcgc acaggtcgat 481 ctgtgtgtgg actgcacaac tggctgcctc aaagggcttg ccaacgtgca gtgttctgac 541 ctgctcaaga agtggctgcc gcaacgctgt gcgacctttg ccagcaagat ccagggccag 601 gtggacaaga tcaagggggc cggtggtgac taagcggccg caataaaata tctttatttt 661 cattacatct gtgtgttggt tttttgtgtg tctagaaata attcttactg tcatgccaag 721 taagatgctt ttctgtgctg caatagcagg catgctgggg atgcggtggg ctctatggct 781 tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc 841 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc 901 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc 961 cgtcaagctc taaatcgggg catcccttta gggttccgat ttagtgcttt acggcacctc 1021 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg 1081 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact 1141 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttggggatt 1201 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt 1261 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccaggcagg cagaagtatg 1321 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca 1381 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact 1441 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta 1501 atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag 1561 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc 1621 attttcggat ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga 1681 ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa 1741 cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt 1801 ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg 1861 ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa 1921 gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac 1981 cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt 2041 gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact 2101 cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg 2161 ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg 2221 acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc 2281 atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt 2341 gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc 2401 gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg 2461 ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg 2521 attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct 2581 ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta 2641 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat 2701 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct 2761 gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt 2821 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag 2881 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt 2941 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag 3001 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg 3061 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat 3121 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta 3181 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa 3241 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc 3301 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt 3361 ccgcctttct cccttcggga agcgtggcgc tttctcaatg ctcacgctgt aggtatctca 3421 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg 3481 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat 3541 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta 3601 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct 3661 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac 3721 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa 3781 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa 3841 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt 3901 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca 3961 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca 4021 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc 4081 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa 4141 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc 4201 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca 4261 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat 4321 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag 4381 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac 4441 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt 4501 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt 4561 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc 4621 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat 4681 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca 4741 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga 4801 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg 4861 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg 4921 ttccgcgcac atttccccga aaagtgccac ctgacgtc //