LOCUS pGPS2.1 4490 bp DNA circular 22-OCT-2007
DEFINITION Transposon donor vector pGPS2.1, complete sequence.
ACCESSION
VERSION
KEYWORDS .
SOURCE Transposon donor vector pGPS2.1
ORGANISM Transposon donor vector pGPS2.1
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 4490)
AUTHORS Biery,M.C., Stewart,F.J., Stellwagen,A.E., Raleigh,E.A. and
Craig,N.L.
TITLE A simple in vitro Tn7-based transposition system with low target
site selectivity for genome and gene analysis
JOURNAL Nucleic Acids Res 28 (5), 1067-1077 (2000)
PUBMED 10666445
REFERENCE 2 (bases 1 to 4490)
AUTHORS Anton,B.P.
TITLE Direct Submission
JOURNAL Submitted (22-OCT-2007) Research Department, New England Biolabs,
240 County Road, Ipswich, MA 01938, USA
FEATURES Location/Qualifiers
source 1..4490
/organism="Transposon donor vector pGPS2.1"
/mol_type="other DNA"
rep_origin complement(369..678)
/note="R6K origin of replication (counter-clockwise)"
rep_origin complement(732..1032)
/note="R6K origin of replication (counter-clockwise)"
gene 1416..2606
/gene="tet"
CDS 1416..2606
/gene="tet"
/note="tetR (confers resistance to tetracycline)"
/codon_start=1
/product="tetracycline efflux protein, class C"
/translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASH
YGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAG
RIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLH
APFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTV
FFIMQLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGE
KQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQG
QLQGSLAALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSR
ATST"
misc_feature 3093..4467
/note="Transprimer-1 transposable element"
misc_feature 3093..3290
/note="Tn7R (right end)"
gene complement(3389..4048)
/gene="cat"
CDS complement(3389..4048)
/gene="cat"
/note="camR (confers resistance to chloramphenicol)"
/codon_start=1
/product="chloramphenicol acetyltransferase"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
A"
misc_feature 4301..4467
/note="Tn7L (left end)"
BASE COUNT 1120 a 1129 c 1072 g 1169 t
ORIGIN
1 ggtaccctgt gaatgcgcaa accaaccctt ggcagaacat atccatcgcg tccgccatct
61 ccagcagccg cacgcggcgc atctcgggca gcgttgggtc ctggccacgg gtgcgcatga
121 tcgtgctcct gtcgttgagg acccggctag gctggcgggg ttgccttact ggttagcaga
181 atgaatcacc gatacgcgag cgaacgtgaa gcgactgctg ctgcaaaacg tctgcgacct
241 gagcaacaac atgaatggtc ttcggtttcc gtgtttcgta aagtctggaa acgcggaagt
301 cagcgccctg caccattatg ttccggatct atgtcgggtg cggagaaaga ggtaatgaaa
361 tggcagatcc ctggcttgtt gtccacaacc gttaaacctt aaaagcttta aaagccttat
421 atattctttt ttttcttata aaacttaaaa ccttagaggc tatttaagtt gctgatttat
481 attaatttta ttgttcaaac atgagagctt agtacgtgaa acatgagagc ttagtacgtt
541 agccatgaga gcttagtacg ttagccatga gggtttagtt cgttaaacat gagagcttag
601 tacgttaaac atgagagctt agtacgtgaa acatgagagc ttagtacgta ctatcaacag
661 gttgaactgc tgatcttcgg atctatgtcg ggtgcggaga aagaggtaat gaaatggcag
721 atccctggct tgttgtccac aaccgttaaa ccttaaaagc tttaaaagcc ttatatattc
781 ttttttttct tataaaactt aaaaccttag aggctattta agttgctgat ttatattaat
841 tttattgttc aaacatgaga gcttagtacg tgaaacatga gagcttagta cgttagccat
901 gagagcttag tacgttagcc atgagggttt agttcgttaa acatgagagc ttagtacgtt
961 aaacatgaga gcttagtacg tgaaacatga gagcttagta cgtactatca acaggttgaa
1021 ctgctgatct tcggatctat gtcgggtgcg gagaaagagg taatgaaatg gcatccggat
1081 ctgcatcgca ggatgctgct ggctaccctg tggaacacct acatctgtat taacgaagca
1141 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
1201 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
1261 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
1321 tcttcaagaa ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat
1381 cacagttaaa ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg
1441 tcatcctcgg caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc
1501 cgggcctctt gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc
1561 tagcgctata tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc
1621 gctttggccg ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga
1681 tcatggcgac cacacccgtc ctgtggatcc tctacgccgg acgcatcgtg gccggcatca
1741 ccggcgccac aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc
1801 gggctcgcca cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg
1861 tggccggggg actgttgggc gccatctcct tgcatgcacc attccttgcg gcggcggtgc
1921 tcaacggcct caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc
1981 gtcgaccgat gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca
2041 tgactatcgt cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc
2101 cggcagcgct ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg acgatgatcg
2161 gcctgtcgct tgcggtattc ggaatcttgc acgccctcgc tcaagccttc gtcactggtc
2221 ccgccaccaa acgtttcggc gagaagcagg ccattatcgc cggcatggcg gccgacgcgc
2281 tgggctacgt cttgctggcg ttcgcgacgc gaggctggat ggccttcccc attatgattc
2341 ttctcgcttc cggcggcatc gggatgcccg cgttgcaggc catgctgtcc aggcaggtag
2401 atgacgacca tcagggacag cttcaaggat cgctcgcggc tcttaccagc ctaacttcga
2461 tcattggacc gctgatcgtc acggcgattt atgccgcctc ggcgagcaca tggaacgggt
2521 tggcatggat tgtaggcgcc gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg
2581 catggagccg ggccacctcg acctgaatgg aagccggcgg cacctcgcta acggattcac
2641 cactccaaga attggagcca atcaattctt gcggagaact gtgaatgcgc aaaccaaccc
2701 ttggcagaac atatccatcg cgtccgccat ctccagcagc cgcacgcggc gcatctcggg
2761 cagcgttggg tcctgggctg gcattgaccc tgagtgattt ttctctggtc ccgccgcatc
2821 cataccgcca gttgtttacc ctcacaacgt tccagtaacc gggcatgttc atcatcagta
2881 acccgtatcg tgagcatcct ctctcgtttc atcggtatca ttacccccat gaacagaaat
2941 cccccttaca cggaggcatc agtgaccaaa caggaaaaaa ccgcccttaa catggcccgc
3001 tttatcagaa gccagacatt aacgcttctg gagaaactca acgagctgga cgcggatgaa
3061 caggcagagc tcttactgtc atgccatccg tatgtgggcg gacaataaag tcttaaactg
3121 aacaaaatag atctaaacta tgacaataaa gtcttaaact agacagaata gttgtaaact
3181 gaaatcagtc cagttatgct gtgaaaaagc atactggact tttgttatgg ctaaagcaaa
3241 ctcttcattt tctgaagtgc aaattgcccg tcgtattaaa gaggggcgtg gggtcgacgc
3301 ggccgctaac tataacggtc ctaaggtagc gagtttaaac gatatcggat ccaggcgttt
3361 aagggcacca ataactgcct taaaaaaatt acgccccgcc ctgccactca tcgcagtact
3421 gttgtaattc attaagcatt ctgccgacat ggaagccatc acaaacggca tgatgaacct
3481 gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc atggtgaaaa
3541 cgggggcgaa gaagttgtcc atattggcca cgtttaaatc aaaactggtg aaactcaccc
3601 agggattggc tgagacgaaa aacatattct caataaaccc tttagggaaa taggccaggt
3661 tttcaccgta acacgccaca tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt
3721 ggtattcact ccagagcgat gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag
3781 ggtgaacact atcccatatc accagctcac cgtctttcat tgccatacgg aattccggat
3841 gagcattcat caggcgggca agaatgtgaa taaaggccgg ataaaacttg tgcttatttt
3901 tctttacggt ctttaaaaag gccgtaatat ccagctgaac ggtctggtta taggtacatt
3961 gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat atatcaacgg
4021 tggtatatcc agtgattttt ttctccattt tagcttcctt agctcctgaa aatctcgata
4081 actcaaaaaa tacgcccggt agtgatctta tttcattatg gtgaaagttg gaacctctta
4141 cgtgccgatc aacgtctcat tttcgccaaa agttggccca gggcttcccg gtatcaacag
4201 ggacaccagg atttatttat tctgcgaagt gatcttccgt cacaggtatg gatccgatat
4261 catttaaatc tagggataac agggtaatac tagtgtcgac caaccagata agtgaaatct
4321 agttccaaac tattttgtca tttttaattt tcgtattagc ttacgacgct acacccagtt
4381 cccatctatt ttgtcactct tccctaaata atccttaaaa actccatttc cacccctccc
4441 agttcccaac tattttgtcc gcccacaccg taagatgctt ttctgtgact
//