LOCUS pGT-N29 4662 bp DNA circular 07-DEC-2007
DEFINITION Cloning vector pGT-N29, complete sequence.
ACCESSION
VERSION
KEYWORDS .
SOURCE Cloning vector pGT-N29
ORGANISM Cloning vector pGT-N29
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 4662)
AUTHORS Polakiewicz,R.D.
TITLE Direct Submission
JOURNAL Submitted (07-DEC-2007) Research Department, New England Biolabs,
240 County Road, Ipswich, MA 01938, USA
FEATURES Location/Qualifiers
source 1..4662
/organism="Cloning vector pGT-N29"
/mol_type="other DNA"
gene 143..1003
/gene="bla"
CDS 143..1003
/gene="bla"
/note="ampR (confers resistance to ampicillin)"
/codon_start=1
/product="beta-lactamase"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERSPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
sig_peptide 143..211
/gene="bla"
/note="Required for secretion to the periplasm; cleaved
off to form the mature beta-lactamase protein."
rep_origin complement(1045..1554)
/note="M13 origin of replication (+ -)"
rep_origin 1665..2253
/note="pMB1 origin of replication (clockwise) (RNAII -35
to RNA/DNA switch point)"
misc_feature 2465..2553
/note="multiple cloning site I (NotI-ApaI)"
promoter 2544..3081
/note="phosphoglycerate kinase (PGK) promoter region"
gene 3082..3885
/gene="aph(3')-II"
CDS 3082..3885
/gene="aph(3')-II"
/note="neoR, kanR, nptII (confers resistance to neomycin
and kanamycin)"
/codon_start=1
/product="aminoglycoside phosphotransferase from Tn5"
/translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSA
QGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPG
QDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDD
LDEEHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVA
DRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 3886..4373
/note="PGK polyA"
misc_feature 4369..4447
/note="multiple cloning site II (XbaI-AflII)"
BASE COUNT 1102 a 1184 c 1238 g 1138 t
ORIGIN
1 gttaactacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
61 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
121 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
181 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
241 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
301 gatccttgag agttttcgcc ccgaagaacg ttctccaatg atgagcactt ttaaagttct
361 gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat
421 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
481 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
541 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
601 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
661 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
721 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
781 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
841 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
901 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
961 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
1021 ctcatatata ctttagattg atttaccccg gttgataatc agaaaagccc caaaaacagg
1081 aagattgtat aagcaaatat ttaaattgta aacgttaata ttttgttaaa attcgcgtta
1141 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
1201 aaatcaaaag aatagcccga gatagggttg agtgttgttc cagtttggaa caagagtcca
1261 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc
1321 ccactacgtg aaccatcacc caaatcaagt tttttggggt cgaggtgccg taaagcacta
1381 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcg aacgtggcga
1441 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca
1501 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtaaaagg
1561 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
1621 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
1681 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
1741 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
1801 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
1861 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
1921 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
1981 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
2041 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
2101 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
2161 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
2221 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
2281 ttcctggcct tttgctggcc ttttgctcac atgtaatgtg agttagctca ctcattaggc
2341 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
2401 acaatttcac acaggaaaca gctatgacca tgattacgcc aagctacgta atacgactca
2461 ctaggcggcc gcctagtggg taccagagct ccctaggttc tagaaccggt gacgtctccc
2521 atggtgaagc ttggatccac gattcgaggg cccctgcagg tcaattctac cgggtagggg
2581 aggcgctttt cccaaggcag tctggagcat gcgctttagc agccccgctg gcacttggcg
2641 ctacacaagt ggcctctggc ctcgcacaca ttccacatcc accggtagcg ccaaccggct
2701 ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag gaagttcccc
2761 cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg tctcactagt
2821 ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg gggcagcggc
2881 caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg tgggtccggg
2941 ggcgggctca ggggcgggct caggggcggg gcgggcgcga aggtcctccc gaggcccggc
3001 attctcgcac gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg
3061 gcctttcgac ctgcagccaa tatgggatcg gccattgaac aagatggatt gcacgcaggt
3121 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
3181 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
3241 accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg
3301 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
3361 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
3421 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
3481 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
3541 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
3601 ttcgccaggc tcaaggcgcg catgcccgac ggcgatgatc tcgtcgtgac ccatggcgat
3661 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
3721 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
3781 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
3841 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagggga tcgatccgtc
3901 ctgtaagtct gcagaaattg atgatctatt aaacaataaa gatgtccact aaaatggaag
3961 tttttcctgt catactttgt taagaagggt gagaacagag tacctacatt ttgaatggaa
4021 ggattggagc tacgggggtg ggggtggggt gggattagat aaatgcctgc tctttactga
4081 aggctcttta ctattgcttt atgataatgt ttcatagttg gatatcataa tttaaacaag
4141 caaaaccaaa ttaagggcca gctcattcct cccactcatg atctatagat ctatagatct
4201 ctcgtgggat cattgttttt ctcttgattc ccactttgtg gttctaagta ctgtggtttc
4261 caaatgtgtc agtttcatag cctgaagaac gagatcagca gcctctgttc cacatacact
4321 tcattctcag tattgttttg ccaagttcta attccatcag aagctgactc tagatcctgc
4381 aggaattcct cgagaccgta cgtgcgcgcg aatgcatcca gatcttccct ctagtcaagg
4441 ccttaagtga gtcgtattac ggactggccg tcgttttaca acgtcgtgac tgggaaaacc
4501 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
4561 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc
4621 gcttcgcttg gtaataaagc ccgcttcggc gggctttttt tt
//