LOCUS pNEBR-R1 10295 bp DNA circular 24-SEP-2008 DEFINITION Cloning vector pNEBR-R1, complete sequence. ACCESSION VERSION KEYWORDS . SOURCE Cloning vector pNEBR-R1 ORGANISM Cloning vector pNEBR-R1 other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 10295) AUTHORS Maina,C.V. TITLE Direct Submission JOURNAL Submitted (24-SEP-2008) Research Department, New England Biolabs, 240 County Road, Ipswich, MA 01938, USA FEATURES Location/Qualifiers source 1..10295 /organism="Cloning vector pNEBR-R1" /mol_type="genomic DNA" gene complement(661..4281) /gene="RheoReceptor" polyA_signal complement(661..1179) /gene="RheoReceptor" /note="TK polyA (counter-clockwise)" CDS complement(1210..2676) /gene="RheoReceptor" /codon_start=1 /product="RheoReceptor" /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKT KRSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNV NKDAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSPEFPGIRPECVVP ETQCAMKRKEKKAQKEKDKLPVSTTTVDDHMPPIMQCEPPPPEAARIHEVVPRFLSDK LLVTNRQKNIPQLTANQQFLIARLIWYQDGYEQPSDEDLKRITQTWQQADDENEESDT PFRQITEMTILTVQLIVEFAKGLPGFAKISQPDQITLLKACSSEVMMLRVARRYDAAS DSILFANNQAYTRDNYRKAGMAEVIEDLLHFCRCMYSMALDNIHYALLTAVVIFSDRP GLEQPQLVEEIQRYYLNTLRIYILNQLSGSARSSVIYGKILSILSELRTLGMQNSNMC ISLKLKNRKLPPFLEEIWDVADMSHTQPPPILESPTNL" promoter complement(2692..4274) /gene="RheoReceptor" /note="UbB promoter (counter-clockwise)" gene 4290..6740 /gene="RheoActivator" promoter 4290..5506 /gene="RheoActivator" /note="UbC promoter (clockwise)" CDS 5522..6496 /gene="RheoActivator" /codon_start=1 /product="RheoActivator" /translation="MGPKKKRKVAPPTDVSLGDELHLDGEDVAMAHADALDDFDLDML GDGDSPGPGFTPHDSAPYGALDMADFEFEQMFTDALGIDEYGGEFEMPVDRILEAELA VEQKSDQGVEGPGGTGGSGSSPNDPVTNICQAADKQLFTLVEWAKRIPHFSSLPLDDQ VILLRAGWNELLIASFSHRSIDVRDGILLATGLHVHRNSAHSAGVGAIFDRVLTELVS KMRDMRMDKTELGCLRAIILFNPEVRGLKSAQEVELLREKVYAALEEYTRTTHPDEPG RFAKLLLRLPSLRSIGLKCLEHLFFFRLIGDVPIDTFLMEMLESPSDS" polyA_signal 6504..6740 /gene="RheoActivator" /note="SV40 late polyA (clockwise)" gene 6747..8063 /gene="aph(3')-II" promoter 6747..7106 /gene="aph(3')-II" /note="SV40 enhancer/early promoter (clockwise)" CDS 7157..7951 /gene="aph(3')-II" /note="neoR, kanR, nptII (confers resistance to neomycin and kanamycin)" /codon_start=1 /product="aminoglycoside phosphotransferase from Tn5" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" polyA_signal 8015..8063 /gene="aph(3')-II" /note="synthetic polyA (clockwise)" rep_origin complement(8551..9239) /note="pUC19 origin of replication (counter-clockwise) (RNAII -35 to RNA/DNA switch point)" gene complement(9310..10170) /gene="bla" CDS complement(9310..10170) /gene="bla" /note="ampR (confers resistance to ampicillin)" /codon_start=1 /product="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" BASE COUNT 2271 a 2738 c 2737 g 2549 t ORIGIN 1 cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg 61 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc 121 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc 181 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta 241 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta 301 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg 361 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa 421 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttccattcgc cattcaggct 481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa 541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg 601 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tgggtacgta 661 ggcggcccgc cagaaatccg cgcggtggtt tttgggggtc gggggtgttt ggcagccaca 721 gacgcccggt gttcgtgtcg cgccagtaca tgcggtccat gcccaggcca tccaaaaacc 781 atgggtctgt ctgctcagtc cagtcgtgga cctgacccca cgcaacgccc aaaataataa 841 cccccacgaa ccataaacca ttccccatgg gggaccccgt ccctaaccca cggggccagt 901 ggctatggca gggcctgccg ccccgacgtt ggctgcgagc cctgggcctt cacccgaact 961 tggggggtgg ggtggggaaa aggaagaaac gcgggcgtat tggccccaat ggggtctcgg 1021 tggggtatcg acagagtgcc agccctggga ccgaaccccg cgtttatgaa caaacgaccc 1081 aacacccgtg cgttttattc tgtcttttta ttgccgtcat agcgcgggtt ccttccggta 1141 ttgtctcctt ccgtgtttca gttagcctcc cccatctccc tgcagcccgg gggatcgaaa 1201 ggcggccgcc tagagattcg tgggggactc gaggataggc ggcggttggg tgtgcgacat 1261 gtccgccaca tcccagatct cctcgaggaa aggcggcagc tttctgttct tgagcttgag 1321 ggagatgcac atgttggagt tttgcatgcc gagcgtgcgt agctcagaga ggattgagag 1381 gatcttgccg tatatgacgg acgaacgcgc cgacccgctc agctggttca ggatatagat 1441 gcggagcgta ttcaggtagt accgctggat ctcttccacc agttgcggct gctccaaccc 1501 tggccggtca gaaaagatga cgacagccgt gagcagcgcg taatggatgt tgtccaacgc 1561 catagagtac atgcaccggc agaagtgcag tagatcctcg atgacctcgg ccatgccagc 1621 cttgcggtag ttgtcgcgag tgtacgcttg gttgttcgcg aacagaatac tgtctgaggc 1681 cgcatcgtat cgtcgcgcga ctcggagcat cattacctca cttgagcaag ccttaagcag 1741 cgtaatttga tcaggctgcg agatcttggc gaaccctggc aatcccttcg cgaactccac 1801 gataagttgg accgtgagga tagtcatctc tgtgatctgg cggaagggag tgtccgactc 1861 ttcgttttca tcgtccgctt gctgccacgt ctgcgtaatc ctcttcaaat cttcatcaga 1921 aggctgctcg tacccgtcct ggtaccagat gagcctggcg ataaggaact gctggttggc 1981 tgtcaactgg gggatgtttt tctgccggtt tgtcaccaac agcttgtcgg agagaaacct 2041 tgggaccact tcgtgaatcc ttgctgcttc aggaggtgga ggttcacact gcataatggg 2101 cggcatgtgg tcgtccaccg tcgtcgtgct gacaggcagt ttgtccttct ccttctgtgc 2161 tttcttctct ttccgcttca tggcgcactg agtctcgggt actacgcact caggccggat 2221 ccccgggaat tccggcgata cagtcaactg tctttgacct ttgttactac tctcttccga 2281 tgatgatgtc gcacttattc tatgctgtct caatgttaga ggcatatcag tctccactga 2341 agccaatcta tctgtgacgg catctttatt cacattatct tgtacaaata atcctgttaa 2401 caatgctttt atatcctgta aagaatccat tttcaaaatc atgtcaaggt cttctcgagg 2461 aaaaatcagt agaaatagct gttccagtct ttctagcctt gattccactt ctgtcagatg 2521 tgccctagtc agcggagacc ttttggtttt gggagagtag cgacactccc agttgttctt 2581 cagacacttg gcgcacttcg gtttttcttt ggagcacttg agctttttaa gtcggcaaat 2641 atcgcatgct tgttcgatag aagacagtag cttcatggtg gcatcgctag ccactaagat 2701 ctgcatttga cctagtttaa aagtaaaaca taagcgtgtc acctcaggat cattgtattt 2761 cttgctttta cacctaatat tcttcaaaat tagcaactat tccttcaaaa tgccttaaat 2821 acgttaatat tccgcaagcc tcccctcaat tttacaaggc taagtcactt aatacgaaat 2881 tcgtcattca aggtttcaac gtcaacagcg gaaaatccag agcgctctct taccaagcgt 2941 ccacagcttt ccccactaac tgttccggat attcaaactc ccccgaccga actccaatct 3001 gcctcaaacc taagcacggt gttaggcaaa ctgaaaacaa aaccaaaccc acaagactcg 3061 cgaaataaaa atcccattga aacacaagaa ggggcacctt tctcacactg ggattccaaa 3121 gtaaaggctt ccagaaacca cacaaactac ctcttttccg ccttccccgc gaagtaaact 3181 cgacagaatc gccgatcggt taggcgtccg ttagtttccg tcaacgcgcg cgcgcgccaa 3241 ccgccctcgc ccccctcagg tcaacaaggc ccactgtccc ggaaaggccc agcgccgcgt 3301 cacctcgccc tcccctcccc cacgccctcc cctcagcggc ccacccaacc ccaagaagcc 3361 tccaccttga cctcacccat tcccccctct cacacccccc caaggtcgtt acggctgcgg 3421 gccagtacct gttagcggat accaggatcc tgccaatcac caaccacgtc cacccacagg 3481 gacacaaaca agctcaccca acaaagccaa ccgcccctaa atgctccggg ctggcggagc 3541 gcaaatttat gcaacgagtg ttgcgtcact tatcacccct cacgtagacg ccgtctccgt 3601 gcgccgctga aagtagttcg ccgcgcccaa cctcccgaga ggcagtttag gcttttcaac 3661 tgagccccaa attcctcata gccgtaagaa aggctcctaa aaaattattt cccttctcct 3721 ctatgcgctt ctcttccttc tttaggaaaa tataagaaaa ttatcaggcg gatgaatttg 3781 agttgaagaa accttcctcg aaagctctgg aaacttcctt gcagcgccgg cactgattgg 3841 ttaggcctgc gaaggcgagg tcggcgctga ttggtggggg acgcggtggc tctgttgttg 3901 ctgggctcca cctggggtgg cttagcgccg aggttgctaa gtaactgagc gcgcgtgcgc 3961 cgcacaggtc tttccttttt tttcttgaga cggagtttcg ctcttgtttc ccaggctgga 4021 gtgcaatggc gcgatctcgg ctcatcgcaa cctccgcctc ccgggttcaa gcgattctcc 4081 tgcctcagcc tcccaagtag ctgggattac aggcatgcgc caccacgtcc ggctaatttt 4141 ttgtattttt agtagagacg ggggtttcac catgtttgtc aggctggtct cgaactcctg 4201 acctcaggta atccacccgc ctcggcctgc caaagtgggc cgccgcgcct cagaagcggg 4261 acggcgtggc acccgatatc gggcgcgccg gcctccgcgc cgggttttgg cgcctcccgc 4321 gggcgccccc ctcctcacgg cgagcgctgc cacgtcagac gaagggcgca gcgagcgtcc 4381 tgatccttcc gcccggacgc tcaggacagc ggcccgctgc tcataagact cggccttaga 4441 accccagtat cagcagaagg acattttagg acgggacttg ggtgactcta gggcactggt 4501 tttctttcca gagagcggaa caggcgagga aaagtagtcc cttctcggcg attctgcgga 4561 gggatctccg tggggcggtg aacgccgatg attatataag gacgcgccgg gtgtggcaca 4621 gctagttccg tcgcagccgg gatttgggtc gcggttcttg tttgtggatc gctgtgatcg 4681 tcacttggtg agtagcgggc tgctgggctg gccggggctt tcgtggccgc cgggccgctc 4741 ggtgggacgg aagcgtgtgg agagaccgcc aagggctgta gtctgggtcc gcgagcaagg 4801 ttgccctgaa ctgggggttg gggggagcgc agcaaaatgg cggctgttcc cgagtcttga 4861 atggaagacg cttgtgaggc gggctgtgag gtcgttgaaa caaggtgggg ggcatggtgg 4921 gcggcaagaa cccaaggtct tgaggccttc gctaatgcgg gaaagctctt attcgggtga 4981 gatgggctgg ggcaccatct ggggaccctg acgtgaagtt tgtcactgac tggagaactc 5041 ggtttgtcgt ctgttgcggg ggcggcagtt atggcggtgc cgttgggcag tgcacccgta 5101 cctttgggag cgcgcgccct cgtcgtgtcg tgacgtcacc cgttctgttg gcttataatg 5161 cagggtgggg ccacctgccg gtaggtgtgc ggtaggcttt tctccgtcgc aggacgcagg 5221 gttcgggcct agggtaggct ctcctgaatc gacaggcgcc ggacctctgg tgaggggagg 5281 gataagtgag gcgtcagttt ctttggtcgg ttttatgtac ctatcttctt aagtagctga 5341 agctccggtt ttgaactatg cgctcggggt tggcgagtgt gttttgtgaa gttttttagg 5401 caccttttga aatgtaatca tttgggtcaa tatgtaattt tcagtgttag actagtaaat 5461 tgtccgctaa attctggccg tttttggctt ttttgttaga cgaatcgcta gcgccgccac 5521 catgggccct aaaaagaagc gtaaagtcgc ccccccgacc gatgtcagcc tgggggacga 5581 gctccactta gacggcgagg acgtggcgat ggcgcatgcc gacgcgctag acgatttcga 5641 tctggacatg ttgggggacg gggattcccc gggtccggga tttacccccc acgactccgc 5701 cccctacggc gctctggata tggccgactt cgagtttgag cagatgttta ccgatgccct 5761 tggaattgac gagtacggtg gggaattcga gatgcctgtg gacaggatcc tggaggcaga 5821 gcttgctgtg gaacagaaga gtgaccaggg cgttgagggt cctgggggaa ccgggggtag 5881 cggcagcagc ccaaatgacc ctgtgactaa catctgtcag gcagctgaca aacagctatt 5941 cacgcttgtt gagtgggcga agaggatccc acacttttcc tccttgcctc tggatgatca 6001 ggtcatattg ctgcgggcag gctggaatga actcctcatt gcctcctttt cacaccgatc 6061 cattgatgtt cgagatggca tcctccttgc cacaggtctt cacgtgcacc gcaactcagc 6121 ccattcagca ggagtaggag ccatctttga tcgggtgctg acagagctag tgtccaaaat 6181 gcgtgacatg aggatggaca agacagagct tggctgcctg agggcaatca ttctgtttaa 6241 tccagaggtg aggggtttga aatccgccca ggaagttgaa cttctacgtg aaaaagtata 6301 tgccgctttg gaagaatata ctagaacaac acatcccgat gaaccaggaa gatttgcaaa 6361 acttttgctt cgtctgcctt ctttacgttc cataggcctt aagtgtttgg agcatttgtt 6421 tttctttcgc cttattggag atgttccaat tgatacgttc ctgatggaga tgcttgaatc 6481 accttctgat tcataatcta gacgagcaga catgataaga tacattgatg agtttggaca 6541 aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc 6601 tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt 6661 tatgtttcag gttcaggggg agatgtggga ggttttttaa agcaagtaaa acctctacaa 6721 atgtggtaaa atccgataag cgtaccttct gaggcggaaa gaaccagctg tggaatgtgt 6781 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc 6841 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta 6901 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc 6961 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta 7021 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct 7081 tttttggagg cctaggcttt tgcaaaaagc ttgattcttc tgacacaaca gtctcgaact 7141 taaggctaga gccaccatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg 7201 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc 7261 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg 7321 tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt 7381 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg 7441 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat 7501 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca 7561 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca 7621 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa 7681 ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa 7741 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc 7801 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga 7861 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc 7921 cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac 7981 caagcgacgc ccaacctgcc atcacgatgg ccgcaataaa atatctttat tttcattaca 8041 tctgtgtgtt ggttttttgt gtgaacgtac gagtcggccg ccaccgcggt ggagctccag 8101 cttttgttcc ctttagtgag ggttaatttc gagcttggcg taatcatggt catagctgtt 8161 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa 8221 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact 8281 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc 8341 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg 8401 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc 8461 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag 8521 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca 8581 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca 8641 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg 8701 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag 8761 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt 8821 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca 8881 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg 8941 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt 9001 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc 9061 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg 9121 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg 9181 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta 9241 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg 9301 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg 9361 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc 9421 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc 9481 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc 9541 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag 9601 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat 9661 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg 9721 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt 9781 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag 9841 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg 9901 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt 9961 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct 10021 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac 10081 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat 10141 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat 10201 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca 10261 aataggggtt ccgcgcacat ttccccgaaa agtgc //