LOCUS pNEBR-X1 4287 bp DNA circular 19-OCT-2007 DEFINITION Cloning vector pNEBR-X1, complete sequence. ACCESSION VERSION KEYWORDS . SOURCE Cloning vector pNEBR-X1 ORGANISM Cloning vector pNEBR-X1 other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 4287) AUTHORS Maina,C.V. TITLE Direct Submission JOURNAL Submitted (19-OCT-2007) Research Department, New England Biolabs, 240 County Road, Ipswich, MA 01938, USA FEATURES Location/Qualifiers source 1..4287 /organism="Cloning vector pNEBR-X1" /mol_type="other DNA" polyA_signal 4..855 /note="SV40 polyA" gene 995..1855 /gene="bla" CDS 995..1855 /gene="bla" /note="ampR (confers resistance to ampicillin)" /codon_start=1 /product="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERSPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin complement(1897..2406) /note="M13 origin of replication (+ -)" rep_origin 2517..3105 /note="pUC19 origin of replication (clockwise) (RNAII -35 to RNA/DNA switch point)" terminator 3167..3625 /note="SV40 transcription terminator; prevents read-through transcription of the expression cassette from sources upstream of 5XRE" gene 3735..4259 /gene="lacZalpha" CDS 3735..4259 /gene="lacZalpha" /codon_start=1 /product="beta-galactosidase alpha fragment" /translation="MTMITPSYGRSTVLRAEYCPPSGVLSSERSTVLRAEFCPPSGDS RGYIMGAVSYYQSSWARAIEAGWRQASLQDIWIHEFLIKAGLERPLAIAARHASPDVQ ACVDPLVKAYSESYYGLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPS QQLRSLNGEWRFAW" promoter 3762..3854 /gene="lacZalpha" /note="5X GAL4 response element (5XRE) (approx. transcript start 3908 clockwise)" TATA_signal 3873..3879 /gene="lacZalpha" /note="minimal TATA box for 5XRE" misc_feature 3910..4068 /gene="lacZalpha" /note="multiple cloning site (ApaI-StuI)" BASE COUNT 1184 a 967 c 977 g 1159 t ORIGIN 1 gttctagagg atctttgtga aggaacctta cttctgtggt gtgacataat tggacaaact 61 acctacagag atttaaagct ctaaggtaaa tataaaattt ttaagtgtat aatgtgttaa 121 actactgatt ctaattgttt gtgtatttta gattccaacc tatggaactt atgaatggga 181 gcagtggtgg aatgccttta atgaggaaaa cctgttttgc tcagaagaaa tgccatctag 241 tgatgatgag gctactgctg actctcaaca ttctactctc aaaagaagag aaaggtagag 301 acccaaggac tttccttcag aattgctaag ttttttgagt catgctgtgt ttagtaatag 361 aactcttgct tgctttgcta tttacaacca caaaggaaaa agctgcactg ctatacaaga 421 aaattatgga aaaatattct gtaaccttta taagtaggca taacagttat aatcataaca 481 tactgttttt tcttactcca cacaggcata gagtgtctgc tattaataac tatgctcaaa 541 aattgtgtac ctttagcttt ttaatttgta aaggggttaa taaggaatat ttgatgtata 601 gtgccttgac tagagatcat aatcagccat accacatttg tagaggtttt acttgcttta 661 aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt 721 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca 781 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct 841 tatcatgtct ggatcaacta cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc 901 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg 961 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc 1021 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt 1081 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct 1141 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttctccaa tgatgagcac 1201 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact 1261 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa 1321 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga 1381 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt 1441 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga 1501 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg 1561 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat 1621 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat 1681 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc 1741 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga 1801 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc 1861 agaccaagtt tactcatata tactttagat tgatttaccc cggttgataa tcagaaaagc 1921 cccaaaaaca ggaagattgt ataagcaaat atttaaattg taaacgttaa tattttgtta 1981 aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc 2041 aaaatccctt ataaatcaaa agaatagccc gagatagggt tgagtgttgt tccagtttgg 2101 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat 2161 cagggcgatg gcccactacg tgaaccatca cccaaatcaa gttttttggg gtcgaggtgc 2221 cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag 2281 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa 2341 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg 2401 gcgcgtaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa 2461 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga 2521 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg 2581 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc 2641 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag 2701 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc 2761 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg 2821 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac 2881 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga 2941 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt 3001 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag 3061 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg 3121 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgtatca gccatatcac 3181 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca 3241 taaaatgaat gcaattgttg ttaacttgtt tattgcagct tataatggtt acaaataaag 3301 caatagcatc acaaatttca caaataaagc atttttcact gcattctagt tgtggtttgt 3361 ccaaactcat caatgtatct tatcatgtct ggatcagcca tatcacattt gtagaggttt 3421 tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa 3481 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa 3541 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca 3601 atgtatctta tcatgtctgc catgtaatgt gagttagctc actcattagg caccccaggc 3661 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca 3721 cacaggaaac agctatgacc atgattacgc caagctacgg gcggagtact gtcctccgag 3781 cggagtactg tcctccgagc ggagtactgt cctccgagcg gagtactgtc ctccgagcgg 3841 agttctgtcc tccgagcgga gactctagag ggtatataat gggggccgtc agctactacc 3901 agagctcatg ggcccgtgca attgaagccg gctggcgcca agcttctctg caggatatct 3961 ggatccacga attcttaatt aaggccggcc tcgagcggcc gctagcgatc gcggcgcgcc 4021 acgcgtctcc ggatgtacag gcatgcgtcg accctctagt caaggcctat agtgagtcgt 4081 attacggact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac 4141 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca 4201 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcttc gcttggtaat 4261 aaagcccgct tcggcgggct ttttttt //