LOCUS pSNAP-tagT7 3725 bp DNA circular 21-JAN-2010 DEFINITION Cloning vector pSNAP-tagT7, complete sequence. ACCESSION VERSION KEYWORDS . SOURCE Cloning vector pSNAP-tagT7 ORGANISM Cloning vector pSNAP-tagT7 other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 3725) AUTHORS Brecht,A. and Muentner,K. TITLE Direct Submission JOURNAL Submitted (21-JAN-2010) Research Department, New England Biolabs, 240 County Road, Ipswich, MA 01938, USA FEATURES Location/Qualifiers source 1..3725 /organism="Cloning vector pSNAP-tagT7" /mol_type="other DNA" rep_origin complement(16..461) /note="f1 origin of replication (+ -)" promoter 629..646 /note="T7 promoter" RBS 714..719 /note="for translation of SNAP26b" gene 728..1303 /gene="SNAP26b" CDS 728..1303 /gene="SNAP26b" /note="optimized for bacterial expression" /codon_start=1 /product="O-6-methylguanine-DNA methyltransferase" /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHEIKLLGKGTSAADAV EVPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKL LKVVKFGEVISYQQLAALAGNPAATAAVKTALSGNPVPILIPCHRVVSSSGAVGGYEG GLAVKEWLLAHEGHRLGKPGLGPAGIGAPGS" misc_feature 1275..1342 /note="multiple cloning site (PstI-XhoI)" terminator 1352..1474 /note="T7 Tphi transcription terminator" promoter complement(1628..1657) /note="Plac promoter (-35 signal TTTACA, -10 signal TATGTT)" rep_origin complement(1981..2569) /note="pUC19 origin of replication (counter-clockwise) (RNAII -35 to RNA/DNA switch point)" gene complement(2740..3600) /gene="bla" CDS complement(2740..3600) /gene="bla" /note="ampR (confers resistance to ampicillin) (mutant V82I,A182V)" /codon_start=1 /product="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" BASE COUNT 924 a 923 c 991 g 887 t ORIGIN 1 cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag 61 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac 121 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga 181 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc 241 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg 301 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa 361 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac 421 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct 481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa 541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg 601 ttgtaaaacg acggccagtg agcgcgcgta atacgactca ctatagggcg aattgggtac 661 cgggcccaga ccacaacggt ttccctctag aaataatttt gtttaacttt aagaaggaga 721 tatacatatg gacaaagatt gcgaaatgaa acgtaccacc ctggatagcc cgctgggcaa 781 actggaactg agcggctgcg aacagggcct gcatgaaatt aaactgctgg gtaaaggcac 841 cagcgcggcc gatgcggttg aagttccggc cccggccgcc gtgctgggtg gtccggaacc 901 gctgatgcag gcgaccgcgt ggctgaacgc gtattttcat cagccggaag cgattgaaga 961 atttccggtt ccggcgctgc atcatccggt gtttcagcag gagagcttta cccgtcaggt 1021 gctgtggaaa ctgctgaaag tggttaaatt tggcgaagtg attagctatc agcagctggc 1081 ggccctggcg ggtaatccgg cggccaccgc cgccgttaaa accgcgctga gcggtaaccc 1141 ggtgccgatt ctgattccgt gccatcgtgt ggttagctct agcggtgcgg ttggcggtta 1201 tgaaggtggt ctggcggtga aagagtggct gctggcccat gaaggtcatc gtctgggtaa 1261 accgggtctg ggacctgcag gtataggcgc gccaggatcc taagaattct gcagatatcc 1321 atcacactgg cggccgctcg agcagatccg gctgctaaca aagcccgaaa ggaagctgag 1381 ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc 1441 ttgaggggtt ttttgctgaa aggaggaact atatccggat aatctagagg cactagttct 1501 agagcggccg ccaccgcggt ggagctccag cttttgttcc ctttagtgag ggttaattgc 1561 gcgcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat 1621 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag 1681 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg 1741 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc 1801 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc 1861 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa 1921 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt 1981 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg 2041 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg 2101 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag 2161 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc 2221 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa 2281 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg 2341 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc 2401 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac 2461 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg 2521 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt 2581 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt 2641 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa 2701 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga 2761 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt 2821 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg 2881 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga 2941 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga 3001 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg 3061 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc 3121 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc 3181 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca 3241 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac 3301 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg 3361 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc 3421 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg 3481 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac 3541 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat 3601 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata 3661 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa 3721 agtgc //