LOCUS pTK-GLuc 5897 bp DNA circular 07-NOV-2008 DEFINITION Cloning vector pTK-GLuc, complete sequence. ACCESSION VERSION KEYWORDS . SOURCE Cloning vector pTK-GLuc ORGANISM Cloning vector pTK-GLuc other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 5897) AUTHORS Shea,C. and Tzertzinis,G. TITLE Direct Submission JOURNAL Submitted (07-NOV-2008) Research Department, New England Biolabs, 240 County Road, Ipswich, MA 01938, USA FEATURES Location/Qualifiers source 1..5897 /organism="Cloning vector pTK-GLuc" /mol_type="other DNA" promoter 18..770 /note="HSV TK promoter (clockwise)" misc_RNA 796..805 /note="Kozak consensus sequence for GLuc" gene 802..1359 /gene="GLuc" CDS 802..1359 /gene="GLuc" /codon_start=1 /product="Gaussia princeps luciferase" /translation="MGVKVLFALICIAVAEAKPTENNEDFNIVAVASNFATTDLDADR GKLPGKKLPLEVLKEMEANARKAGCTRGCLICLSHIKCTPKMKKFIPGRCHTYEGDKE SAQGGIGEAIVDIPEIPGFKDLEPMEQFIAQVDLCVDCTTGCLKGLANVQCSDLLKKW LPQRCATFASKIQGQVDKIKGAGGD" misc_feature 1360..1391 /note="MCS (NotI-XbaI)" polyA_signal 1392..1622 /note="SV40 polyA" promoter 2215..2550 /note="SV40 promoter (clockwise)" gene 2602..3396 /gene="aph(3')-II" CDS 2602..3396 /gene="aph(3')-II" /note="neoR, kanR, nptII (confers resistance to neomycin and kanamycin)" /codon_start=1 /product="aminoglycoside phosphotransferase from Tn5" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" promoter complement(3789..3818) /note="Plac promoter (-35 signal TTTACA, -10 signal TATGTT)" rep_origin complement(4142..4730) /note="pUC19 origin of replication (counter-clockwise) (RNAII -35 to RNA/DNA switch point) (old sequence errors)" gene complement(4901..5761) /gene="bla" CDS complement(4901..5761) /gene="bla" /note="ampR (confers resistance to ampicillin) (mutant V82I,A182V)" /codon_start=1 /product="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" BASE COUNT 1336 a 1553 c 1600 g 1408 t ORIGIN 1 gacggatcgg gagatctaaa tgagtcttcg gacctcgcgg gggccgctta agcggtggtt 61 agggtttgtc tgacgcgggg ggagggggaa ggaacgaaac actctcattc ggaggcggct 121 cggggtttgg tcttggtggc cacgggcacg cagaagagcg ccgcgatcct cttaagcacc 181 cccccgccct ccgtggaggc gggggtttgg tcggcgggtg gtaactggcg ggccgctgac 241 tcgggcgggt cgcgcgcccc agagtgtgac cttttcggtc tgctcgcaga cccccgggcg 301 gcgccgccgc ggcggcgacg ggctcgctgg gtcctaggct ccatggggac cgtatacgtg 361 gacaggctct ggagcatccg cacgactgcg gtgatattac cggagacctt ctgcgggacg 421 agccgggtca cgcggctgac gcggagcgtc cgttgggcga caaacaccag gacggggcac 481 aggtacacta tcttgtcacc cggaggcgcg agggactgca ggagcttcag ggagtggcgc 541 agctgcttca tccccgtggc ccgttgctcg cgtttgctgg cggtgtcccc ggaagaaata 601 tatttgcatg tctttagttc tatgatgaca caaaccccgc ccagcgtctt gtcattggcg 661 aattcgaaca cgcagatgca gtcggggcgg cgcggtccca ggtccacttc gcatattaag 721 gtgacgcgtg tggcctcgaa caccgagcga ccctgcagcg acccgcttaa aagcttggta 781 ccgagctcgg atccagccac catgggagtc aaagttctgt ttgccctgat ctgcatcgct 841 gtggccgagg ccaagcccac cgagaacaac gaagacttca acatcgtggc cgtggccagc 901 aacttcgcga ccacggatct cgatgctgac cgcgggaagt tgcccggcaa gaagctgccg 961 ctggaggtgc tcaaagagat ggaagccaat gcccggaaag ctggctgcac caggggctgt 1021 ctgatctgcc tgtcccacat caagtgcacg cccaagatga agaagttcat cccaggacgc 1081 tgccacacct acgaaggcga caaagagtcc gcacagggcg gcataggcga ggcgatcgtc 1141 gacattcctg agattcctgg gttcaaggac ttggagccca tggagcagtt catcgcacag 1201 gtcgatctgt gtgtggactg cacaactggc tgcctcaaag ggcttgccaa cgtgcagtgt 1261 tctgacctgc tcaagaagtg gctgccgcaa cgctgtgcga cctttgccag caagatccag 1321 ggccaggtgg acaagatcaa gggggccggt ggtgactaag cggccgcacc ggtctcgagc 1381 atgcatctag attcgagcag acatgataag atacattgat gagtttggac aaaccacaac 1441 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt 1501 aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca 1561 ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtaa 1621 aatcgataaa ctagaaataa ttcttactgt catgccaagt aagatgcttt tctgtgctgc 1681 aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc 1741 tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg 1801 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct 1861 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggc 1921 atccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag 1981 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg 2041 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc 2101 tcggtctatt cttttgattt ataagggatt ttggggattt cggcctattg gttaaaaaat 2161 gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt 2221 gtggaaagtc cccaggctcc ccaggcaggc agaagtatgc aaagcatgca tctcaattag 2281 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg 2341 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact 2401 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag 2461 gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc 2521 ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcaagag 2581 acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc 2641 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat 2701 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg 2761 tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg 2821 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta 2881 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta 2941 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc 3001 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc 3061 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg 3121 ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg 3181 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt 3241 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc 3301 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc 3361 atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga 3421 ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg 3481 aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg 3541 atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca 3601 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt 3661 gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct 3721 agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa 3781 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga 3841 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt 3901 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct 3961 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat 4021 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga 4081 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt 4141 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt 4201 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc 4261 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa 4321 gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct 4381 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta 4441 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg 4501 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc 4561 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta 4621 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg 4681 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt 4741 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg 4801 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta 4861 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg 4921 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg 4981 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc 5041 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg 5101 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg 5161 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag 5221 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat 5281 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc 5341 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc 5401 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa 5461 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac 5521 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt 5581 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc 5641 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa 5701 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca 5761 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat 5821 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa 5881 aagtgccacc tgacgtc //