NEBuilder HiFi DNA Assembly offers several advantages over GeneArt Gibson Assembly and In-Fusion Snap Assembly. These include: higher accuracy due to the use of a high-fidelity polymerase, the ability to assemble both 5´- and 3´-end mismatches, lower DNA input requirements and the ability to bridge two dsDNA fragments with a ssDNA oligo. NEBuilder HiFi is the clear choice for efficient and accurate DNA assembly.
See how NEBuilder HiFi outperforms GeneArt Gibson Assembly and In-Fusion Snap Assembly
- Improved thermostability
- 2-Fragment assembly
- 4-Fragment assembly
- 6-Fragment assembly
- ssDNA oligo with linearized vector
- Annealed ssDNA oligos with 3´ and 5´ overhangs to a linearized vector
- Amplification of 12-fragment DNA assembly product
- In vitro enrichment of assembled DNA by phi-29 DNA Polymerase
One or more of these products are covered by patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc. For more information, please email us at [email protected]. The use of these products may require you to obtain additional third party intellectual property rights for certain applications.
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