The ability to synthesize DNA from an RNA template, via reverse transcription, enables researchers to study RNA with the same molecular approaches used for DNA investigations. cDNA generated by reverse transcription can be amplified using polymerase chain reaction (PCR). The combination of reverse transcription and PCR (RT-PCR) permits sensitive detection of low abundance RNAs in a sample. The resultant PCR Products can be used in many downstream applications, such as cloning into plasmid vectors and sequencing using next generation sequencing platforms.

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Protocols for RT-PCR