The presence of intrinsic PCR inhibitors in some sample types, such as those from blood or fixed tissues, can prevent the direct amplification of DNA from these samples. DNA can be purified prior to PCR via a variety of extraction techniques but these methods can be costly, time intensive and result in significant sample loss. Extraction-free PCR typically benefits from a polymerase that is resistant to inhibitors, allowing for robust amplification of source DNA without additional extraction steps.
Anatomy of a Polymerase - How Structure Effects Function
Polymerase Fidelity: What is it, and what does it mean for your PCR?
Understanding Variability in DNA Amplification Reactions
- DNA Polymerase Selection Chart
- PCR Troubleshooting Guide
- Taq PCR Kit Troubleshooting Guide
- Choosing RNA Input Amounts for NEB T2010
- General Guidelines for Successful RNA Purification Using the Monarch Total RNA Miniprep Kit
- Guidelines for PCR Optimization with OneTaq® and OneTaq® Hot Start DNA Polymerases
- Guidelines for PCR Optimization with Taq DNA Polymerase
- Guidelines for PCR Optimization with Thermophilic DNA Polymerases
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