Epigenetics

Bisulfite Conversion

Sodium bisulfite conversion of genomic DNA to differentiate and detect unmethylated versus methylated cytosines is the gold standard for DNA methylation analysis. It provides single nucleotide resolution map of 5-mC of the genome (1). Bisulfite conversion involves the deamination of unmodified cytosines to uracil, leaving the modified bases 5-mC and 5-hmC. This procedure can then be followed either by PCR amplification or massively parallel sequencing methods to reveal the methylation status of every cytosine in gene specific amplification or whole genome amplification.

Treatment of denatured DNA (i.e., single-stranded DNA) with sodium bisulfite leads to deamination of unmethylated cytosine residues to uracil, leaving 5-mC or 5-hmC intact. The uracils are amplified in subsequent PCR reaction as thymines, whereas 5-mC or 5-hmC residues get amplified as cytosines. Comparison of sequence information between the reference genome and bisulfite-treated DNA can provide information about cytosine methylation patterns. Other applications, such as Methylation-Specific PCR (MSP), involve analyzing untreated and bisulfite treated DNA using two different sets of PCR primer pairs, one pair that targets the unaltered, methylated sequence and the other that targets the converted unmethylated sequence.

A new method for methylome analysis at the single base level, NEBNext® Enzymatic Methyl-seq (EM-seq™) (NEB #E7120), is now available. This enzyme-based technology minimizes damage to DNA and produces high-quality libraries that enable superior detection of 5mC and 5hmC from fewer sequencing reads. 

 
  1. Frommer et al. (1992) Proc. Natl. Acad. Sci. 89. 1827-1831. PMID: 1542678

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FAQs for Bisulfite Conversion
Protocols for Bisulfite Conversion
Application Notes Bisulfite Conversion
    Publications related to Bisulfite Conversion
  1. Chernov AV., Reyes L., Peterson S., Strongin AY. 2015. Depletion of CG-Specific Methylation in Mycoplasma hyorhinis Genomic DNA after Host Cell Invasion PLoS One. 10, PubMedID: 26544880, DOI: 10.1371/journal.pone.0142529
Overview of EpiMark® Bisulfite Conversion Kit
Overview of NEBNext® Enzymatic Methyl-seq
NEBNext_EM-seq_Fig1_EM-seqConversion
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