
Methylated DNA Analysis
Choose Type:
- Is S-adenosylmethionine (SAM) supplied with the Methyltransferase?
- Will all the sites in the DNA become methylated by SssI Methyltransferase?
- What is the molecular weight of SssI (CpG) Methyltransferase?
- Can DNA be radiolabeled with SssI Methyltransferase?
- Can SssI methylated DNA be used to transform E. coli?
- What is the activity of SssI Methyltransferase in other NEBuffers, including CutSmart?
- Does SssI Methyltransferase require magnesium in the buffer?
- What should be considered if the methylation using SssI Methyltransferase is not going to completion?
- How does Dnmt1 differ from SssI methylase?
- Can the SssI Methyltransferase methylate single stranded DNA?
- Can SssI Methyltransferase be used for generating a positive control for methylation-specific PCR or bisulfate sequencing?
- What is the specific activity of SssI Methyltransferase?
- Capture and Elute Step for Control DNA (E2600)
- Capture Methylated CpG DNA (E2600)
- Cross-linking of IgG to Protein A or G Beads
- Cycling Protocol Using EpiMark™ Bisulfite Conversion Kit (E3318)
- DNA Fragmentation (E2600)
- Double Digest Protocol with Standard Restriction Enzymes
- Downstream Analysis (NEB #E2600)
- Elute Captured Methylated CpG DNA (E2600)
- End-point PCR Using EpiMark Bisulfite Conversion Kit (E3318)
- EpiMark® Hot Start Taq DNA Polymerase Guidelines for PCR (M0490)
- Immunoprecipitation using Protein A/G Magnetic Beads
- Loop-mediated Isothermal Amplification (LAMP)
- mRNA Isolation using Streptavidin Magnetic Beads (NEB #S1420 and NEB #S1421)
- Optimizing Restriction Endonuclease Reactions
- Phage Display: Solution-phase Panning with Affinity Bead Capture
- Prebind MBD2a-Fc to Protein A Magnetic Beads (NEB #E2600)
- Protocol for generating 32 bp fragments from modified CpG sites in genomic DNA using FspEI
- Protocol for generating 32 bp fragments from modified CpG sites in genomic DNA using LpnPI (R0663)
- Protocol for generating 32 bp fragments from modified CpG sites in genomic DNA using MspJI
- Protocol for Glucosylation and digestion of Genomic DNA using AbaSI (#R0665)
- Protocol for use with NEBNext ChIP-seq Library Prep Reagent Set for Illumina (E6200)
- Purification of IgG using Protein A/G Magnetic Beads
- Reaction Protocol for EpiMark® 5-hmC and 5-mC Analysis Kit (E3317)
- Reagent Preparation Using EpiMark® Bisulfite Conversion Kit (E3318)
- Wash Off Unbound DNA (E2600)
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Epigenetics - Expanding on Genomic Foundations
- Epigenetics Brochure
Feature Articles
Brochures
- Woodcock, C.B.l, Yu, D., Hajian, T., Li, J., Huang, Y., Dai, N., Corrêa, I.R., Jr., Wu, T., Vedadi, M., Zhang, X., Cheng, X 2019. Human MettL3 MettL14 complex is a sequence specific DNA adenine methyltransferase active on single strand and unpaired DNA in vitro Cell Discov. 5:63, PubMedID: 31885874, DOI: 10.1038/s41421-019-0136-4
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