Epi_App_Hero

Histones & Nucleosome Assembly

Chromatin is the macromolecular state in which nuclear DNA is packaged within the cell. Nucleosomes are the structural units of chromatin. Each nucleosome is composed of ~147 bp double stranded DNA and a histone octamer which contains two copies of each core histone (H2A, H2B, H3 and H4). Histone H1, a linker histone, is involved in binding between nucleosomes to further compact the chromatin. The striking features of these nucleosomal histones are their amino terminus tails which are often modified by various histone modifying enzymes. The predominate modifications on histone tails include but are not limited to methylation, acetylation and phosphorylation. Histone modifications are key to transcription, replication and DNA repair functions (1, 2).

 
  1. Luger, Karolin et al. (1997) Nature 389(6648):251-60. PMID: 9305837
  2. Chakravarthy, Srinivas et al. (2005) FEBS Lett. 579(4):895-8 PMID: 15680970

Choose Type:

FAQs for Histones & Nucleosome Assembly
Protocols for Histones & Nucleosome Assembly
Legal Information

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.


Videos

  • High Sensitivity 5-hmC Detection in Balb/C Brain Tissue

    High Sensitivity 5-hmC Detection in Balb/C Brain Tissue

    Follow NEB Product Development Scientist, Romas Vaisvila, Ph.D., as he demonstrates the EpiMark® 5-mc and 5-hmc Analysis Kit for locus specific identification and quantification of 5-hmc in this protocol "High Sensitivity 5-hmc detection in Balb/C Brain Tissue".

  • What is Epigenetics?

    What Is Epigenetics?

    If all cells are created from the same genetic material, why are there so many different cell types? Listen to Sriharsa Pradhan, Senior Scientist, RNA Biology at NEB, as he describes how DNA is methylated and how this affects the path of reading the DNA code the same way an obstruction would derail a train off its tracks.