A widely used method to identify mutations is the T7 Endonuclease I mutation detection assay (1,2). This assay detects heteroduplex DNA that results from the annealing of a DNA strand, including desired mutations, with a wild-type DNA strand. The EnGen Mutation Detection Kit provides optimized reagents for performing robust T7 Endonuclease-based detection of genome editing events.
- Reyon, D. et al. (2012) Nat. Biotechnol., 30(5):460-5
- Vouillot, L., et al. (2015) G3 (Bethesda), 5(3):407-15