The biology of carbohydrates, or glycobiology, is attracting growing attention in high impact research areas (neurobiology, development, immunology), and also in applied fields such as manufacturing of biotherapeutics and antibodies, and heparin analogs. Carbohydrates attached to glycolipids or glycoproteins determine the properties and fates of living cells, therefore the structural characterization of glycans and glycosylated proteins plays a crucial role in elucidating central biological processes.
Glycoproteins and other glycoconjugates are structurally diverse and virtually impossible to predict from genetic information alone. Analytical tools, including specific enzymes such as endoglycosidases and exoglycosidases, along with chromatography, mass spectrometry methods and specialized bioinformatics, allow us to define the composition of glycoconjugates in any given model system. However, descriptive analysis only gives a snapshot of the biological process of interest. In many cases, purifying a target from a recombinant expression system is the preferred method to understand the functional properties of a glycoprotein.
- How can I separate a deglycosylated protein from the glycosidase?
- What are glycosidases and their uses?
- What are the best techniques to analyze N- and O-glycans?
- What are the advantages of enzymatic deglycosylation?
- Can glycosidases be used in combination for extensive digestion?
- Do I need to deglycosylate my protein sample for proteomic analysis?
- Can I analyze protein glycosylation with only benchtop instrumentation and reagents?
- Is it possible to predict whether a protein is N- or O-glycosylated?
- Which proteins are glycosylated?
Glycomics: A rapidly evolving field with a sweet future
Glycans are widely distributed in nature and have physical, chemical, and biological properties that make them important players in areas such as biofuels, food, materials science, biotechnology, and pharmaceuticals.
N-Glycan Composition Profiling for Quality Testing of Biotherapeutics
N-Glycan Composition Profiling for Quality Testing of Biotherapeutics
The Structure, Function and Importance of Carbohydrates
Using Glycosidases to Remove Trim or Modify Glycans on Therapeutic Protein
The Glycoproteomics brochure provides information on the suite of endo- and exoglycosidases, and deglycosylation enzymes offered by NEB.
Glycoproteomics Technical Guide
Find in-depth information, including protocols, technical tips, frequently asked questions and application notes, utilizing NEB’s suite of endo- and exoglycosidases.
Rapid PNGase F Trifold
Learn how Rapid PNGase F can speed up your antibody deglycosylation step and make your therapeutic proteins faster and more efficiently than ever before.
- Improving Sample Workflow: Rapid PNGase F for Accurate Antibody Characterization (2015)
- Glycan Analyzer
- Endoglycosidase Selection Chart
- Detailed Characterization of Several Glycosidase Enzymes
- Glycobiology Unit Conversion Chart
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Learn about glycobiology and its importance in clinical and diagnostic applications in this episode of NEB TV. Also, hear more about how NEB is setting the bar for product quality in this rapidly growing field.
Learn about the core sequences and common modifications of N-linked and O-linked glycans in this video. Analysis of these glycans can be accomplished with the use of deglycosylation enzymes, which can provide complete sugar removal with no protein degradation.