The DNA repair step is easily integrated into sequencing workflows for any platform, upstream of library preparation, and treatment results in both increased library yields and increased library quality.
Table 1: Types of FFPE DNA damage and their ability to be repaired by the NEBNext FFPE DNA Repair Mix
|FFPE DAMAGE TYPE||REPAIRED BY THE FFPE DNA ENZYME REPAIR MIX?|
|Deamination of cytosine to uracil||Yes|
|Nicks and gaps||Yes|
|Blocked 3´ ends||Yes|
Protocols for NEBNext FFPE DNA Repair Mix
- NEBNext FFPE DNA Repair Mix (M6630) - Protocol for use with Other User-supplied Library Construction Reagents (M6630)
- Protocol for use with NEBNext FFPE DNA Repair Mix (#M6630), NEBNext DNA Library Prep Master Mix Set for Illumina (NEB #E6040)
- Protocol for use with NEBNext Ultra DNA Library Prep Kit for Illumina (E7370)
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.