Advantages of automation include:
- Increased throughput
- Improved reproducibility
- Increased speed
- Reduced hands-on time
- Reduced Repetitive Strain Injury (RSI) incidence
- Fewer components simplify automation setup
- Streamlined workflows minimize tip consumption and chances for pipetting errors
- Less clean-up bead consumption results in a lower cost-per-reaction
- Low input amount capability (ng for DNA/RNA, pg for ChIP) improves sequencing results with less sample
- Robust reagents improve overall library yields
- Sufficient kit component fill volumes for automation protocols improves usability
ION TORRENT™ is a trademark of Life Technologies, Inc.
BECKMAN COULTER® and BIOMEK® are registered trademarks of Beckman Coulter, Inc.
HAMILTON® is a registered trademark and STARLETTM is a trademark of the Hamilton company.
PERKINELMER® is a registered trademark of PerkinElmer, Inc.
ILLUMINA® is a registered trademark of Illumina, Inc.
AGILENT® AND TAPESTATION® are registered trademarks of Agilent, Inc.
Application Notes NEBNext® Automation
- Automated Directional RNASeq Library Preparation using the Biomek FXP Liquid Handler
- Automated DNA Library Construction Using the NEBNext Ultra DNA Library Prep Kit for Illumina® on the Beckman Coulter Biomek FXP Automated Liquid Handler
- Automated Library Preparation using NEBNext® Ultra™ DNA Library Prep Kit for Illumina® (E7370) on the HAMILTON® STAR™ Line
- Automated size selection of NEBNext Small RNA libraries with the Sage Pippin Prep
- Flexible Preparation of High Quality NGS DNA Libraries with the NEBNext Ultra II DNA Library Prep Kit on the Tecan® Freedom Evo® NGS Workstation
Learn how Biomek automation of NEBNext RNA library preparation enables high-throughput, reproducible production of libraries with high yield and complexity. In this webinar Zach Smith from Beckman Coulter describes strand-specific RNA library preparation using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina and discusses Biomek automation of this method.
Learn how Biomek automation of NEBNext DNA library preparation for Ion Torrent enables high-throughput, reproducible production of libraries with high yield and complexity. In this webinar Joanna Hamilton from Dartmouth Genomics & Molecular Biology Shared Resource, Lynne Apone from New England Biolabs and Zach Smith from Beckman Coulter describe the NEBNext Fast Fragmentation & DNA Library Prep kit for Ion Torrent, automation of this method and implementation in multiple workflows in a sequencing core lab.