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NEBNext® Sample Preparation for Next Generation Sequencing

NEBNext® reagents are a series of highly pure reagents that facilitate sample preparation of DNA or RNA for next generation sequencing, and target enrichment of DNA. Available for a wide range of sample types, NEBNext products are provided in user-friendly formats including kits and modules, with bulk or customized formats also available. Adaptors and primers are available separately, for maximum flexibility. These robust reagents undergo stringent quality controls and functional validation, ensuring maximum yield, convenience and value.

Use the following tool to determine the best NEBNext products for you:

NEBNext® Sample Preparation for Next Generation Sequencing

NEBNext Direct® BRCA1/BRCA2 Panel

NEBNext Direct® Cancer HotSpot Panel

NEBNext® Multiplex Oligos for Illumina® (96 Index Primers)

NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 3)

NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 4)

NEBNext® Multiplex Small RNA Library Prep Kit for Illumina® (Index Primers 1-48)

NEBNext® rRNA Depletion Kit (Human/Mouse/Rat) with RNA Sample Purification Beads

NEBNext® Ultra™ II DNA Library Prep Kit for Illumina®

NEBNext® Ultra II DNA Library Prep with Sample Purification Beads

NEBNext® Ultra II FS DNA Library Prep with Sample Purification Beads

NEBNext® Ultra™ II Ligation Module 

NEBNext® Ultra™ II Q5® Master Mix

NEBNext® Ultra™ II End Repair/dA-Tailing Module

Illumina® Library Preparation

NEBNext Direct® BRCA1/BRCA2 Panel

NEBNext Direct® Cancer HotSpot Panel

NEBNext® ChIP-Seq Library Prep Master Mix Set for Illumina®

NEBNext® ChIP-Seq Library Prep Reagent Set for Illumina®

NEBNext® dA-Tailing Module

NEBNext® DNA Library Prep Reagent Set for Illumina®

NEBNext® End Repair Module

NEBNext® FFPE DNA Repair Mix

NEBNext® Library Dilution Buffer

NEBNext® Magnesium RNA Fragmentation Module

NEBNext® mRNA Library Prep Master Mix Set for Illumina®

NEBNext® mRNA Library Prep Reagent Set for Illumina®

NEBNext® Multiplex Oligos for Illumina® (96 Index Primers)

NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 2)

NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 3)

NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 4)

NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 1)

NEBNext® Multiplex Oligos for Illumina® (Methylated Adaptor, Index Primers Set 1)

NEBNext® Multiplex Small RNA Library Prep Kit for Illumina® (Index Primers 1-48)

NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (Set 1)

NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (Set 2)

NEBNext® Multiplex Oligos for Illumina® (Dual Index Primers Set 1)

NEBNext® Poly(A) mRNA Magnetic Isolation Module

NEBNext® Q5® Hot Start HiFi PCR Master Mix

NEBNext® Quick Ligation Module

NEBNext® RNA First Strand Synthesis Module

NEBNext® RNase III RNA Fragmentation Module

NEBNext® rRNA Depletion Kit (Human/Mouse/Rat) with RNA Sample Purification Beads

NEBNext® rRNA Depletion Kit (Human/Mouse/Rat)

NEBNext® Singleplex Oligos for Illumina®

NEBNext® Small RNA Library Prep Set for Illumina® (Multiplex Compatible)

NEBNext® Ultra II Non-Directional RNA Second Strand Synthesis Module

NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina®

NEBNext® Ultra™ End Repair/dA-Tailing Module

NEBNext® Ultra™ II Directional RNA Second Strand Synthesis Module

NEBNext® Ultra™ II DNA Library Prep Kit for Illumina®

NEBNext® Ultra II DNA Library Prep with Sample Purification Beads

NEBNext® Ultra II FS DNA Library Prep Kit for Illumina

NEBNext® Ultra II FS DNA Library Prep with Sample Purification Beads

NEBNext® Ultra II FS DNA Module

NEBNext® Ultra™ II Ligation Module 

NEBNext® Ultra™ II Q5® Master Mix

NEBNext® Ultra™ II End Repair/dA-Tailing Module

NEBNext® Ultra™ Ligation Module

NEBNext® Ultra™ DNA Library Prep Kit for Illumina®

NEBNext® Ultra™ RNA Library Prep Kit for Illumina®

NEBNext® DNA Library Prep Master Mix Set for Illumina®

NEBNext® Library Quant Kit for Illumina®

NEBNext® Magnesium RNA Fragmentation Module

NEBNext® mRNA Library Prep Master Mix Set for Illumina®

NEBNext® mRNA Library Prep Reagent Set for Illumina®

NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 1)

NEBNext® Multiplex Oligos for Illumina® (Methylated Adaptor, Index Primers Set 1)

NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (Set 1)

NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (Set 2)

NEBNext® Poly(A) mRNA Magnetic Isolation Module

NEBNext® RNA First Strand Synthesis Module

NEBNext® RNase III RNA Fragmentation Module

NEBNext® Singleplex Oligos for Illumina®

NEBNext® Small RNA Library Prep Set for Illumina® (Multiplex Compatible)

NEBNext® Ultra II Non-Directional RNA Second Strand Synthesis Module

NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina®

NEBNext® Ultra™ II Directional RNA Second Strand Synthesis Module

NEBNext® Ultra™ RNA Library Prep Kit for Illumina®

NEBNext® ChIP-Seq Library Prep Master Mix Set for Illumina®

NEBNext® ChIP-Seq Library Prep Reagent Set for Illumina®

NEBNext® dA-Tailing Module

NEBNext® DNA Library Prep Reagent Set for Illumina®

NEBNext® End Repair Module

NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 2)

NEBNext® Multiplex Oligos for Illumina® (Dual Index Primers Set 1)

NEBNext® Quick Ligation Module

NEBNext® Ultra™ End Repair/dA-Tailing Module

NEBNext® Ultra II FS DNA Library Prep Kit for Illumina

NEBNext® Ultra II FS DNA Library Prep with Sample Purification Beads

NEBNext® Ultra II FS DNA Module

NEBNext® Ultra™ Ligation Module

NEBNext® Ultra™ DNA Library Prep Kit for Illumina®

NEBNext® DNA Library Prep Master Mix Set for Illumina®

NEBNext® Ultra™ II DNA Library Prep Kit for Illumina®

NEBNext® Ultra II DNA Library Prep with Sample Purification Beads

NEBNext® Ultra™ II Ligation Module 

NEBNext® Ultra™ II Q5® Master Mix

NEBNext® Ultra™ II End Repair/dA-Tailing Module

NEBNext® Ultra II Non-Directional RNA Second Strand Synthesis Module

NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina®

NEBNext® Ultra II Directional RNA Library Prep with Sample Purification Beads

NEBNext® Ultra™ II Directional RNA Second Strand Synthesis Module

NEBNext® Ultra II RNA First Strand Synthesis Module

NEBNext® Ultra II RNA Library Prep Kit for Illumina®

NEBNext® Ultra II RNA Library Prep with Sample Purification Beads

Ion Torrent™ DNA Library Preparation

NEBNext® Fast DNA Fragmentation & Library Prep Set for Ion Torrent™

NEBNext® Fast DNA Library Prep Set for Ion Torrent™

NEBNext® FFPE DNA Repair Mix

NEBNext® Q5® Hot Start HiFi PCR Master Mix

454™ Library Preparation

NEBNext® End Repair Module

NEBNext® Magnesium RNA Fragmentation Module

NEBNext® Poly(A) mRNA Magnetic Isolation Module

NEBNext® Quick Ligation Module

NEBNext® RNase III RNA Fragmentation Module

NEBNext® rRNA Depletion Kit (Human/Mouse/Rat)

NEBNext® Ultra II Non-Directional RNA Second Strand Synthesis Module

NEBNext® DNA Library Prep Master Mix Set for 454™

NEBNext® End Repair Module

NEBNext® Quick Ligation Module

NEBNext® DNA Library Prep Master Mix Set for 454™

NEBNext® Magnesium RNA Fragmentation Module

NEBNext® Poly(A) mRNA Magnetic Isolation Module

NEBNext® RNase III RNA Fragmentation Module

NEBNext® Ultra II Non-Directional RNA Second Strand Synthesis Module

DNA Library Preparation

NEBNext Direct® BRCA1/BRCA2 Panel

NEBNext Direct® Cancer HotSpot Panel

NEBNext® dA-Tailing Reaction Buffer

NEBNext® End Repair Reaction Buffer

NEBNext® FFPE DNA Repair Mix

NEBNext® High-Fidelity 2X PCR Master Mix

NEBNext® Library Dilution Buffer

NEBNext® Multiplex Oligos for Illumina® (96 Index Primers)

NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 3)

NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 4)

NEBNext® Q5® Hot Start HiFi PCR Master Mix

NEBNext® Quick Ligation Reaction Buffer

NEBNext® Ultra™ II DNA Library Prep Kit for Illumina®

NEBNext® Ultra II DNA Library Prep with Sample Purification Beads

NEBNext® Ultra II FS DNA Library Prep Kit for Illumina

NEBNext® Ultra II FS DNA Library Prep with Sample Purification Beads

NEBNext® Ultra II FS DNA Module

NEBNext® Ultra™ II Ligation Module 

NEBNext® Ultra™ II Q5® Master Mix

NEBNext® Ultra™ II End Repair/dA-Tailing Module

NEBNext® Library Quant Kit for Illumina®

RNA Library Preparation

5´ DNA Adenylation Kit

NEBNext® Library Dilution Buffer

NEBNext® Multiplex Small RNA Library Prep Kit for Illumina® (Index Primers 1-48)

NEBNext® Q5® Hot Start HiFi PCR Master Mix

NEBNext® rRNA Depletion Kit (Human/Mouse/Rat) with RNA Sample Purification Beads

NEBNext® rRNA Depletion Kit (Human/Mouse/Rat)

NEBNext® Second Strand Synthesis (dNTP-free) Reaction Buffer

NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina®

NEBNext® Ultra II Directional RNA Library Prep with Sample Purification Beads

NEBNext® Ultra II RNA First Strand Synthesis Module

NEBNext® Ultra II RNA Library Prep Kit for Illumina®

NEBNext® Ultra II RNA Library Prep with Sample Purification Beads

NEBNext® Library Quant Kit for Illumina®

DNA Fragmentation

NEBNext® Fast DNA Fragmentation & Library Prep Set for Ion Torrent™

NEBNext® Ultra II FS DNA Library Prep Kit for Illumina

NEBNext® Ultra II FS DNA Library Prep with Sample Purification Beads

NEBNext® Ultra II FS DNA Module

FFPE DNA Repair

NEBNext® Automation

Illumina® Library Preparation

NEBNext Direct® BRCA1/BRCA2 Panel

NEBNext Direct® Cancer HotSpot Panel

NEBNext® ChIP-Seq Library Prep Master Mix Set for Illumina®

NEBNext® ChIP-Seq Library Prep Reagent Set for Illumina®

NEBNext® dA-Tailing Module

NEBNext® DNA Library Prep Reagent Set for Illumina®

NEBNext® End Repair Module

NEBNext® FFPE DNA Repair Mix

NEBNext® Library Dilution Buffer

NEBNext® Magnesium RNA Fragmentation Module

NEBNext® mRNA Library Prep Master Mix Set for Illumina®

NEBNext® mRNA Library Prep Reagent Set for Illumina®

NEBNext® Multiplex Oligos for Illumina® (96 Index Primers)

NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 2)

NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 3)

NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 4)

NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 1)

NEBNext® Multiplex Oligos for Illumina® (Methylated Adaptor, Index Primers Set 1)

NEBNext® Multiplex Small RNA Library Prep Kit for Illumina® (Index Primers 1-48)

NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (Set 1)

NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (Set 2)

NEBNext® Multiplex Oligos for Illumina® (Dual Index Primers Set 1)

NEBNext® Poly(A) mRNA Magnetic Isolation Module

NEBNext® Q5® Hot Start HiFi PCR Master Mix

NEBNext® Quick Ligation Module

NEBNext® RNA First Strand Synthesis Module

NEBNext® RNase III RNA Fragmentation Module

NEBNext® rRNA Depletion Kit (Human/Mouse/Rat) with RNA Sample Purification Beads

NEBNext® rRNA Depletion Kit (Human/Mouse/Rat)

NEBNext® Singleplex Oligos for Illumina®

NEBNext® Small RNA Library Prep Set for Illumina® (Multiplex Compatible)

NEBNext® Ultra II Non-Directional RNA Second Strand Synthesis Module

NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina®

NEBNext® Ultra™ End Repair/dA-Tailing Module

NEBNext® Ultra™ II Directional RNA Second Strand Synthesis Module

NEBNext® Ultra™ II DNA Library Prep Kit for Illumina®

NEBNext® Ultra II DNA Library Prep with Sample Purification Beads

NEBNext® Ultra II FS DNA Library Prep Kit for Illumina

NEBNext® Ultra II FS DNA Library Prep with Sample Purification Beads

NEBNext® Ultra II FS DNA Module

NEBNext® Ultra™ II Ligation Module 

NEBNext® Ultra™ II Q5® Master Mix

NEBNext® Ultra™ II End Repair/dA-Tailing Module

NEBNext® Ultra™ Ligation Module

NEBNext® Ultra™ DNA Library Prep Kit for Illumina®

NEBNext® Ultra™ RNA Library Prep Kit for Illumina®

NEBNext® DNA Library Prep Master Mix Set for Illumina®

NEBNext® Library Quant Kit for Illumina®

RNA for Illumina®

NEBNext® Magnesium RNA Fragmentation Module

NEBNext® mRNA Library Prep Master Mix Set for Illumina®

NEBNext® mRNA Library Prep Reagent Set for Illumina®

NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 1)

NEBNext® Multiplex Oligos for Illumina® (Methylated Adaptor, Index Primers Set 1)

NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (Set 1)

NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (Set 2)

NEBNext® Poly(A) mRNA Magnetic Isolation Module

NEBNext® RNA First Strand Synthesis Module

NEBNext® RNase III RNA Fragmentation Module

NEBNext® Singleplex Oligos for Illumina®

NEBNext® Small RNA Library Prep Set for Illumina® (Multiplex Compatible)

NEBNext® Ultra II Non-Directional RNA Second Strand Synthesis Module

NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina®

NEBNext® Ultra™ II Directional RNA Second Strand Synthesis Module

NEBNext® Ultra™ RNA Library Prep Kit for Illumina®

DNA for Illumina®

NEBNext® ChIP-Seq Library Prep Master Mix Set for Illumina®

NEBNext® ChIP-Seq Library Prep Reagent Set for Illumina®

NEBNext® dA-Tailing Module

NEBNext® DNA Library Prep Reagent Set for Illumina®

NEBNext® End Repair Module

NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 2)

NEBNext® Multiplex Oligos for Illumina® (Dual Index Primers Set 1)

NEBNext® Quick Ligation Module

NEBNext® Ultra™ End Repair/dA-Tailing Module

NEBNext® Ultra II FS DNA Library Prep Kit for Illumina

NEBNext® Ultra II FS DNA Library Prep with Sample Purification Beads

NEBNext® Ultra II FS DNA Module

NEBNext® Ultra™ Ligation Module

NEBNext® Ultra™ DNA Library Prep Kit for Illumina®

NEBNext® DNA Library Prep Master Mix Set for Illumina®

NEBNext® Ultra™ II DNA Library Prep

NEBNext® Ultra™ II DNA Library Prep Kit for Illumina®

NEBNext® Ultra II DNA Library Prep with Sample Purification Beads

NEBNext® Ultra™ II Ligation Module 

NEBNext® Ultra™ II Q5® Master Mix

NEBNext® Ultra™ II End Repair/dA-Tailing Module

NEBNext® Ultra™ II RNA Library Prep

NEBNext® Ultra II Non-Directional RNA Second Strand Synthesis Module

NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina®

NEBNext® Ultra II Directional RNA Library Prep with Sample Purification Beads

NEBNext® Ultra™ II Directional RNA Second Strand Synthesis Module

NEBNext® Ultra II RNA First Strand Synthesis Module

NEBNext® Ultra II RNA Library Prep Kit for Illumina®

NEBNext® Ultra II RNA Library Prep with Sample Purification Beads

Ion Torrent™ DNA Library Preparation

NEBNext® Fast DNA Fragmentation & Library Prep Set for Ion Torrent™

NEBNext® Fast DNA Library Prep Set for Ion Torrent™

NEBNext® FFPE DNA Repair Mix

NEBNext® Q5® Hot Start HiFi PCR Master Mix

DNA for Ion Torrent™

NEBNext® Fast DNA Fragmentation & Library Prep Set for Ion Torrent™

NEBNext® Fast DNA Library Prep Set for Ion Torrent™

NEBNext® FFPE DNA Repair Mix

NEBNext® Q5® Hot Start HiFi PCR Master Mix

454™ Library Preparation

NEBNext® End Repair Module

NEBNext® Magnesium RNA Fragmentation Module

NEBNext® Poly(A) mRNA Magnetic Isolation Module

NEBNext® Quick Ligation Module

NEBNext® RNase III RNA Fragmentation Module

NEBNext® rRNA Depletion Kit (Human/Mouse/Rat)

NEBNext® Ultra II Non-Directional RNA Second Strand Synthesis Module

NEBNext® DNA Library Prep Master Mix Set for 454™

DNA for 454™

NEBNext® End Repair Module

NEBNext® Quick Ligation Module

NEBNext® DNA Library Prep Master Mix Set for 454™

RNA for 454™

NEBNext® Magnesium RNA Fragmentation Module

NEBNext® Poly(A) mRNA Magnetic Isolation Module

NEBNext® RNase III RNA Fragmentation Module

NEBNext® Ultra II Non-Directional RNA Second Strand Synthesis Module

DNA Library Preparation

NEBNext Direct® BRCA1/BRCA2 Panel

NEBNext Direct® Cancer HotSpot Panel

NEBNext® dA-Tailing Reaction Buffer

NEBNext® End Repair Reaction Buffer

NEBNext® FFPE DNA Repair Mix

NEBNext® High-Fidelity 2X PCR Master Mix

NEBNext® Library Dilution Buffer

NEBNext® Multiplex Oligos for Illumina® (96 Index Primers)

NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 3)

NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 4)

NEBNext® Q5® Hot Start HiFi PCR Master Mix

NEBNext® Quick Ligation Reaction Buffer

NEBNext® Ultra™ II DNA Library Prep Kit for Illumina®

NEBNext® Ultra II DNA Library Prep with Sample Purification Beads

NEBNext® Ultra II FS DNA Library Prep Kit for Illumina

NEBNext® Ultra II FS DNA Library Prep with Sample Purification Beads

NEBNext® Ultra II FS DNA Module

NEBNext® Ultra™ II Ligation Module 

NEBNext® Ultra™ II Q5® Master Mix

NEBNext® Ultra™ II End Repair/dA-Tailing Module

NEBNext® Library Quant Kit for Illumina®

RNA Library Preparation

5´ DNA Adenylation Kit

NEBNext® Library Dilution Buffer

NEBNext® Multiplex Small RNA Library Prep Kit for Illumina® (Index Primers 1-48)

NEBNext® Q5® Hot Start HiFi PCR Master Mix

NEBNext® rRNA Depletion Kit (Human/Mouse/Rat) with RNA Sample Purification Beads

NEBNext® rRNA Depletion Kit (Human/Mouse/Rat)

NEBNext® Second Strand Synthesis (dNTP-free) Reaction Buffer

NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina®

NEBNext® Ultra II Directional RNA Library Prep with Sample Purification Beads

NEBNext® Ultra II RNA First Strand Synthesis Module

NEBNext® Ultra II RNA Library Prep Kit for Illumina®

NEBNext® Ultra II RNA Library Prep with Sample Purification Beads

NEBNext® Library Quant Kit for Illumina®

DNA Fragmentation

NEBNext® Fast DNA Fragmentation & Library Prep Set for Ion Torrent™

NEBNext® Ultra II FS DNA Library Prep Kit for Illumina

NEBNext® Ultra II FS DNA Library Prep with Sample Purification Beads

NEBNext® Ultra II FS DNA Module

FFPE DNA Repair

NEBNext FFPE DNA Repair Mix

NEBNext® Automation