Home Applications NGS Sample Prep & Target Enrichment

NGS Sample Prep & Target Enrichment

High-quality, high-yield library preparation is a critical first step towards optimizing your next generation sequencing (NGS) workflows. The NEBNext® product line is a series of highly pure reagents for both DNA and RNA sample prep, as well as target enrichment of DNA for NGS. NEBNext kits are carefully designed and QC’d to improve yields and library diversity, using a broad range of input amounts from a wide variety of sample types. Recently, a novel enzyme-based DNA fragmentation system was added to enable streamlined DNA fragmentation, end repair, and dA tailing with a single enzyme mix; this kit, the NEBNext Ultra II FS DNA Library Prep Kit (NEB #E7805), enables reliably high-quality and high-yield library construction with as little as 100 pg of input DNA.

For those looking for a more targeted approach, NEBNext provides NEBNext Direct® for target enrichment. This unique hybridization-based technology enables high-sensitivity variant calling with a speed and precision previously only feasible using multiplex PCR-based approaches. For convenient customization, NEBNext Direct Custom Ready Panels offer ready-made, optimized baits for over 850 targets, enabling faster time to targeted results. For sequence-based target genotyping applications in plant, animal and human, the NEBNext Genotyping Solution enables pre-capture pooling of up to 96 samples for cost-effective and scalable genotyping of up to 5,000 genomic markers.

Videos

NEBNEXT^® Ultra II Directional RNA Workflow

Learn more about the streamlined workflow for the NEBNext Ultra II Directional RNA Library Prep Kit.
Size Selection and Cleanup with NEBNEXT^® Ultra II and SPRI beads

This video walks you through the size selection and cleanup steps using the NEBNext Ultra II DNA
Tips for Optimizing DNA Inputs in NGS Library Construction

Optimizing DNA inputs for NGS library prep is an important step, if you want to ensure a high-quality library. Start by following these tips for DNA quantification and assessing purity.
Tips for Optimizing RNA Inputs in NGS Library Construction

Watch as Deyra explains how to optimize your RNA inputs for successful NGS library preparation.
NEBNEXT DIRECT^® Workflow

View our tutorial for help visualizing NEBNext Direct’s unique and enabling workflow.
NEBNEXT DIRECT^® Target Enrichment

Learn about the innovative NEBNext Direct technology for target enrichment for next generation sequencing.
How Library Preparation Affects Sequencing Accuracy

In this webinar, Laurence Etwiller and Jennifer Ong discuss their recent publications, and how library preparation affects sequencing accuracy and variant calling. View full Q & A summary.
Behind the paper: DNA damage is a pervasive cause of sequencing errors, directly confounding variant identification

NEB researchers published a paper in Science highlighting DNA damage as a prevalent source of errors in public cancer databases. Learn about how addressing this damage could improve the detection of low-frequency disease variants.
Challenges and Opportunities for Next Generation Sequencing Target Enrichment

In this webinar, we explore the advantages and limitations of target enrichment, and discuss how NEBNext Direct^®, a novel solution for selective enrichment of genomic targets, addresses these challenges.
NEBNEXT^® Ultra II DNA Library Prep Protocol

This video walks you through DNA Library Preparation using the NEBNext Ultra II DNA Library Prep Kit. The video also includes tips for optimization as well as safe stopping points.
12 Quick Tips for NGS Library Preparation

These 12 quick tips for NGS library preparation are a great refresher if you're a seasoned pro, or a great introduction if you're a beginner.