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Protein Labeling (SNAP/CLIP)
SNAP- and CLIP-tag protein labeling systems enable the specific, covalent attachment of virtually any molecule to a protein of interest. There are two steps to using this system: cloning and expression of the protein of interest as a SNAP-tag® fusion, and labeling of the fusion with the SNAP-tag substrate of choice. The SNAP-tag is a small protein based on human O6-alkylguanine-DNA-alkyltransferase (hAGT), a DNA repair protein. SNAP-tag substrates are dyes, fluorophores, biotin, or beads conjugated to guanine or chloropyrimidine leaving groups via a benzyl linker. In the labeling reaction, the substituted benzyl group of the substrate is covalently attached to the SNAP-tag. CLIP-tag™ is a modified version of SNAP-tag, engineered to react with benzylcytosine rather than benzylguanine derivatives. When used in conjunction with SNAP-tag, CLIP-tag enables the orthogonal and complementary labeling of two proteins simultaneously in the same cells.
Choose Type:
- Cellular Labeling (E9100)
- Cellular Labeling (E9120)
- Cellular Labeling (E9230)
- Cellular Labeling (S9102)
- Cellular Labeling (S9103)
- Cellular Labeling (S9104)
- Cellular Labeling (S9105)
- Cellular Labeling (S9107)
- Cellular Labeling (S9109)
- Cellular Labeling (S9110)
- Cellular Labeling (S9112)
- Cellular Labeling (S9124)
- Cellular Labeling (S9129)
- Cellular Labeling (S9132)
- Cellular Labeling (S9134)
- Cellular Labeling (S9136)
- Cellular Labeling (S9159)
- Cellular Labeling (S9217)
- Cellular Labeling (S9219)
- Cellular Labeling (S9221)
- Cellular Labeling (S9232)
- Cellular Labeling (S9233)
- Cellular Labeling (S9234)
- Cloning of CLIP-tag Fusions in pCLIPf (N9215)
- Cloning of SNAP-tag Fusions in pSNAPf (N9183)
- Cloning of SNAP-tag Fusions in pSNAP-tag(T7)-2 (N9181)
- CoA 488 (S9348)
- Expression of CLIP-tag Fusions (N9215)
- Expression of SNAPf Fusions (N9183)
- Expression of SNAP-tag Fusions (N9181)
- Instructions for Cellular Labeling (E9200)
- Instructions for Labeling of Proteins in vitro (S9348)
- Instructions for Use with SFP Synthase (P9302)
- Instructions for Use with ACP Synthase (P9301)
- Labeling CLIP-tag Fusion Proteins with BC-substrates (S9236)
- Labeling Mammalian Cell Lysates (S9147)
- Labeling of Proteins in vitro (S9107)
- Labeling of Proteins in vitro (S9110)
- Labeling of Proteins in vitro (S9220)
- Labeling of Proteins in vitro (S9233)
- Labeling of Proteins in vitro (S9234)
- Labeling of Proteins in vitro (P9301)
- Labeling of Proteins in vitro (P9302)
- Labeling of Proteins in vitro (S9103)
- Labeling of Proteins in vitro (S9104)
- Labeling of Proteins in vitro (S9105)
- Labeling of Proteins in vitro (S9106)
- Labeling of Proteins in vitro (S9109)
- Labeling of Proteins in vitro (S9143)
- Labeling of Proteins in vitro (S9217)
- Labeling of Proteins in vitro (S9219)
- Labeling of Proteins in vitro (S9221)
- Labeling of Proteins in vitro (S9232)
- Labeling of Proteins in vitro (S9349)
- Labeling of Proteins in vitro (S9350)
- Labeling of Proteins in vitro (S9351)
- Labeling of Proteins in Solution (E9230)
- Labeling of Proteins in vitro (E9100)
- Labeling of Proteins in vitro (S9102)
- Labeling of Proteins in vitro (S9159)
- Labeling on the Surface of Cells (S9349)
- Labeling on the Surface of Cells (S9350)
- Labeling on the Surface of Cells (S9351)
- Labeling Proteins in vitro (S9132)
- Labeling Proteins in vitro (S9147)
- Labeling Proteins in vitro (S9112)
- Labeling Proteins in vitro (S9129)
- Labeling Proteins in vitro (S9134)
- Labeling Proteins in vitro (S9136)
- Labeling Proteins in vitro (E9120)
- Labeling Proteins in vitro (E9200)
- Labeling Proteins in vitro (S9124)
- Labeling SNAP-tag Fusion Proteins with BG-substrates (S9148)
- Labeling SNAP-tag Fusion Proteins with BG-substrates (S9150)
- Labeling SNAP-tag Fusion Proteins with BG-substrates (S9151)
- Labeling SNAP-tag Purified Protein In Vitro (P9312)
- Protocol for Anti-SNAP-tag® Antibody (Polyclonal) (P9310)
- Protocol for Labeling SNAP-tag Fusion Proteins with BG-substrates (S9153)
- Reaction Conditions for Chemical Coupling (S9148)
- Reaction Conditions for Chemical coupling (S9150)
- Reaction Conditions for Chemical Coupling (S9151)
- Reaction Conditions for Chemical Coupling (S9153)
- Reaction Conditions for Chemical Coupling (S9236)
- Use of CLIP-Cell Block with CLIP-Cell Substrates (E9230)
- Use of SNAP-Cell Block with SNAP-Cell Substrates (E9100)
- Use SNAP-Capture Magnetic Beads (S9145)
- Use SNAP-Capture Pull Down Resin (S9144)
- Use with CLIP-tag substrates (S9220)
- Use with SNAP-Cell Substrates (S9106)
- Use with SNAP-Surface substrates (S9143)
- View the video "Fluorescent Labeling of COS-7 Expressing SNAP-tag Fusion Proteins for Live Cell Imaging" in the Journal of Visualized Experiments (JoVE)
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SNAP-tag® Technologies: Tools to Study Protein Function
Read about the NEB’s set of protein tools for the specific labeling (SNAP-, CLIP-, ACP- and MCP-tags) of fusion proteins.
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Cellular Imaging & Analysis Brochure
The Cellular Imaging and Analysis brochure provides information on the labeling technologies offered by NEB for studying the function and localization of proteins in cells.
- Comparison of SNAP-tag®/CLIP-tag™ Technologies to GFP
- Labeling with SNAP-tag® Technology Troubleshooting Guide
Feature Articles
Brochures
Selection Tools
Troubleshooting Guides
- Clone and express once, then use with a variety of substrates
- Non-toxic to living cells
- Wide selection of fluorescent substrates
- Highly specific covalent labeling
- Simultaneous dual protein labeling inside live cells
- Protein localization and translocation
- Pulse-chase experiments
- Receptor internalization studies
- Selective cell surface labeling
- Protein pull-down assays
- Protein detection in SDS-PAGE
- Flow cytometry
- High throughput binding assays in microtiter plates
- Biosensor interaction experiments
- FRET-based binding assays
- Single molecule labeling
- Super-resolution microscopy
While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.
For more information about commercial rights, please contact NEB's Global Business Development team at gbd@neb.com.
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
Videos
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Fluorescent Labeling of COS-7 Expressing SNAP-tag Fusion Proteins for Live Cell Imaging
Watch as Chris Provost, of New England Biolabs, performs fluorescent imaging of live COS-7 cells expressing SNAP-tag® fusion proteins.
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SNAP-tag Overview Tutorial
View an interactive tutorial explaining the mechanism of our SNAP-tag® technologies and reagents available for researchers wishing to study the function and localization of proteins in live or fixed cells.
