Immunoprecipitation (IP) is a method for selectively isolating a particular protein species from a complex protein solution. The technique relies on antigen/antibody recognition (immuno) and the subsequent mass- or affinity-related isolation of the capture/target complex (precipitation). It is the precipitation of the target protein that is referenced by the name: Pull-down assay. Pull-down assays are commonly used to either confirm the presence of a scant protein species or enrich population for a particular protein, as well as to identify natural binding partners for the captured protein. A typical pull-down assay involves the use of a matrix-associated antibody, such as a bead, and various wash and elution steps to ensure the specific preference for the targeted protein of interest.
Protocols for Pull-down Assays
- Cross-linking of IgG to Protein A or G Beads
- Goat Anti-Mouse IgG Magnetic Beads Protocol
- Immunoprecipitation using Protein A/G Magnetic Beads
- Isolation of MBP-fusion protein using Amylose Magnetic Beads
- Phage Display: Solution-phase Panning with Affinity Bead Capture
- Purification of IgG using Protein A/G Magnetic Beads
- Use SNAP-Capture Magnetic Beads (S9145)
- Use SNAP-Capture Pull Down Resin (S9144)
Cellular Imaging & Analysis Brochure
The Cellular Imaging and Analysis brochure provides information on the labeling technologies offered by NEB for studying the function and localization of proteins in cells.
Protein Expression & Purification Brochure
The Protein Expression and Purification brochure provides information on the advanced tools for protein expression and purification offered by NEB.
- Affinity of Protein A/G for IgG Types from Different Species