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Protein Expression and Purification

New England Biolabs is committed to providing advanced tools for protein expression and purification. As a leading supplier for reagents in the biotechnology field, NEB understands the importance of convenient and easy-to-use systems for high level expression and purification of proteins. We invite you to review our growing range of expression systems resulting from our 30 years of experience in cloning, over-expression and purification.

Protein Expression & Purification

Nuclease-free Water

Protein Expression Approaches

Anti-GLuc Antibody

BL21 Competent E. coli

E. coli Ribosome

Enterokinase, light chain

Furin

Amylose Magnetic Beads

Amylose Resin

Amylose Resin High Flow

Anti-CBD Monoclonal Antibody

Anti-MBP Monoclonal Antibody

Anti-MBP Monoclonal Antibody (HRP conjugated)

BL21 Competent E. coli

BL21(DE3) Competent E. coli

Enterokinase, light chain

Furin

IMPACT™ Kit

Lemo21(DE3) Competent E. coli

NEB® 10-beta Electrocompetent E. coli

NEB® 5-alpha Electrocompetent E. coli

NEB® Express Competent E. coli (High Efficiency)

NEB® Express Iq Competent E. coli (High Efficiency)

NEB® Turbo Electrocompetent E. coli

NiCo21(DE3) Competent E. coli

pMAL™ Protein Fusion and Purification System

pMAL™-c5X Vector

pMAL™-p5X Vector

pSNAP-tag® (T7)-2 Vector

pTXB1 Vector

pTYB21 Vector

SHuffle® Express Competent E. coli

SHuffle® T7 Competent E. coli

SHuffle® T7 Express Competent E. coli

SHuffle® T7 Express lysY Competent E. coli

T7 Express Competent E. coli (High Efficiency)

T7 Express Crystal Competent E. coli (High Efficiency)

T7 Express Iq Competent E. coli (High Efficiency)

T7 Express lysY Competent E. coli (High Efficiency)

T7 Express lysY/Iq Competent E. coli (High Efficiency)

K. lactis GG799 Competent Cells

K. lactis Protein Expression Kit

pCLuc Mini-TK 2 Vector

pCLuc-Basic 2 Vector

pCMV-CLuc 2 Control Plasmid

pKLAC2 Vector

pSV40-CLuc Control Plasmid

pTK-CLuc Vector

pTK-GLuc Vector

pTYB21 Vector

Yeast Carbon Base Medium Powder

E. coli Ribosome

pTYB21 Vector

PURExpress® In Vitro Protein Synthesis Kit

PURExpress® Δ (aa, tRNA) Kit

PURExpress® Δ RF123 Kit

PURExpress® Δ Ribosome Kit

RNase Inhibitor, Murine

Coupled Protein Expression & Purification

Amylose Resin

Amylose Resin High Flow

BL21 Competent E. coli

NEB® Express Competent E. coli (High Efficiency)

NEB® Express Iq Competent E. coli (High Efficiency)

pMAL™ Protein Fusion and Purification System

BL21(DE3) Competent E. coli

IMPACT™ Kit

Lemo21(DE3) Competent E. coli

NiCo21(DE3) Competent E. coli

T7 Express Competent E. coli (High Efficiency)

T7 Express Iq Competent E. coli (High Efficiency)

T7 Express lysY Competent E. coli (High Efficiency)

T7 Express lysY/Iq Competent E. coli (High Efficiency)

NiCo21(DE3) Competent E. coli

Chitin Resin

K. lactis Protein Expression Kit

Expression of Difficult Proteins

SHuffle® T7 Competent E. coli

SHuffle® T7 Express Competent E. coli

SHuffle® T7 Express lysY Competent E. coli

Lemo21(DE3) Competent E. coli

PURExpress® In Vitro Protein Synthesis Kit

T7 Express Iq Competent E. coli (High Efficiency)

T7 Express lysY Competent E. coli (High Efficiency)

T7 Express lysY/Iq Competent E. coli (High Efficiency)

Lemo21(DE3) Competent E. coli

T7 Express lysY/Iq Competent E. coli (High Efficiency)

PURExpress® In Vitro Protein Synthesis Kit

SHuffle® Express Competent E. coli

SHuffle® T7 Competent E. coli

SHuffle® T7 Express Competent E. coli

SHuffle® T7 Express lysY Competent E. coli

Affinity Purification

12-Tube Magnetic Separation Rack

50 ml Magnetic Separation Rack

6-Tube Magnetic Separation Rack

96-Well Microtiter Plate Magnetic Separation Rack

Amylose Magnetic Beads

Anti-MBP Magnetic Beads

Chitin Magnetic Beads

Chitin Resin

Goat Anti-Mouse IgG Magnetic Beads

Goat Anti-Rabbit IgG Magnetic Beads

Goat Anti-Rat IgG Magnetic Beads

Hydrophilic Streptavidin Magnetic Beads

pMAL™ Protein Fusion and Purification System

Protein A Magnetic Beads

Protein G Magnetic Beads

SNAP-Capture Magnetic Beads

Streptavidin Magnetic Beads

Protein Expression Approaches

Anti-GLuc Antibody

BL21 Competent E. coli

E. coli Ribosome

Enterokinase, light chain

Furin

E.coli Protein Expression

Amylose Magnetic Beads

Amylose Resin

Amylose Resin High Flow

Anti-CBD Monoclonal Antibody

Anti-MBP Monoclonal Antibody

Anti-MBP Monoclonal Antibody (HRP conjugated)

BL21 Competent E. coli

BL21(DE3) Competent E. coli

Enterokinase, light chain

Furin

IMPACT™ Kit

Lemo21(DE3) Competent E. coli

NEB® 10-beta Electrocompetent E. coli

NEB® 5-alpha Electrocompetent E. coli

NEB® Express Competent E. coli (High Efficiency)

NEB® Express Iq Competent E. coli (High Efficiency)

NEB® Turbo Electrocompetent E. coli

NiCo21(DE3) Competent E. coli

pMAL™ Protein Fusion and Purification System

pMAL™-c5X Vector

pMAL™-p5X Vector

pSNAP-tag® (T7)-2 Vector

pTXB1 Vector

pTYB21 Vector

SHuffle® Express Competent E. coli

SHuffle® T7 Competent E. coli

SHuffle® T7 Express Competent E. coli

SHuffle® T7 Express lysY Competent E. coli

T7 Express Competent E. coli (High Efficiency)

T7 Express Crystal Competent E. coli (High Efficiency)

T7 Express Iq Competent E. coli (High Efficiency)

T7 Express lysY Competent E. coli (High Efficiency)

T7 Express lysY/Iq Competent E. coli (High Efficiency)

Yeast Protein Expression

K. lactis GG799 Competent Cells

K. lactis Protein Expression Kit

pCLuc Mini-TK 2 Vector

pCLuc-Basic 2 Vector

pCMV-CLuc 2 Control Plasmid

pKLAC2 Vector

pSV40-CLuc Control Plasmid

pTK-CLuc Vector

pTK-GLuc Vector

pTYB21 Vector

Yeast Carbon Base Medium Powder

Cell-Free Protein Expression

E. coli Ribosome

pTYB21 Vector

PURExpress® In Vitro Protein Synthesis Kit

PURExpress® Δ (aa, tRNA) Kit

PURExpress® Δ RF123 Kit

PURExpress® Δ Ribosome Kit

RNase Inhibitor, Murine

Coupled Protein Expression & Purification

Maltose Binding Protein Expression

Amylose Resin

Amylose Resin High Flow

BL21 Competent E. coli

NEB® Express Competent E. coli (High Efficiency)

NEB® Express Iq Competent E. coli (High Efficiency)

pMAL™ Protein Fusion and Purification System

IMPACT™ Protein Expression

BL21(DE3) Competent E. coli

IMPACT™ Kit

Lemo21(DE3) Competent E. coli

NiCo21(DE3) Competent E. coli

T7 Express Competent E. coli (High Efficiency)

T7 Express Iq Competent E. coli (High Efficiency)

T7 Express lysY Competent E. coli (High Efficiency)

T7 Express lysY/Iq Competent E. coli (High Efficiency)

His-tagged Protein Expression

NiCo21(DE3) Competent E. coli

Yeast Chitin Binding Domain Tag

Chitin Resin

K. lactis Protein Expression Kit

Expression of Difficult Proteins

SHuffle® T7 Competent E. coli

SHuffle® T7 Express Competent E. coli

SHuffle® T7 Express lysY Competent E. coli

Toxic Protein Expression

Lemo21(DE3) Competent E. coli

PURExpress® In Vitro Protein Synthesis Kit

T7 Express Iq Competent E. coli (High Efficiency)

T7 Express lysY Competent E. coli (High Efficiency)

T7 Express lysY/Iq Competent E. coli (High Efficiency)

Membrane Protein Expression

Lemo21(DE3) Competent E. coli

T7 Express lysY/Iq Competent E. coli (High Efficiency)

Disulfide-Bonded Protein Expression

PURExpress® In Vitro Protein Synthesis Kit

SHuffle® Express Competent E. coli

SHuffle® T7 Competent E. coli

SHuffle® T7 Express Competent E. coli

SHuffle® T7 Express lysY Competent E. coli

Affinity Purification

12-Tube Magnetic Separation Rack

50 ml Magnetic Separation Rack

6-Tube Magnetic Separation Rack

96-Well Microtiter Plate Magnetic Separation Rack

Amylose Magnetic Beads

Anti-MBP Magnetic Beads

Chitin Magnetic Beads

Chitin Resin

Goat Anti-Mouse IgG Magnetic Beads

Goat Anti-Rabbit IgG Magnetic Beads

Goat Anti-Rat IgG Magnetic Beads

Hydrophilic Streptavidin Magnetic Beads

pMAL™ Protein Fusion and Purification System

Protein A Magnetic Beads

Protein G Magnetic Beads

SNAP-Capture Magnetic Beads

Streptavidin Magnetic Beads