NEBExpress® Cell-free E. coli Protein Synthesis System

The NEBExpress® Cell-free E. coli Protein Synthesis System is an extract-based transcription/translation system derived from E. coli cells that have been engineered for high in vitro synthesis performance. It is designed to synthesize proteins ranging in size from 17-230 kDa in just 2-4 hours under the control of T7 RNA Polymerase.

  • Synthesize high yields of protein (typically 0.5 mg/ml)
  • Protein can be synthesized and visualized in approximately 2 - 4 hours
  • Synthesize various target proteins ranging from 17 to 230 kDa
  • Templates can be plasmid DNA, linear DNA or mRNA
  • Minimal protease activity ensures stability of desired target protein
  • RNase contamination can be inhibited by the supplied RNase inhibitor, eliminating clean-up steps
  • Protein can be isolated with affinity purification or subject to direct functional analysis
  • Reactions can be directly loaded onto an SDS-PAGE gel without the need for acetone or TCA precipitation
  • Flexible reaction conditions achieve maximum yield; protein synthesis can be sustained for 10 hours at 37°C or up to 24 hours at lower temperatures
  • Reactions can be miniaturized or scaled up
  • Compatible with the PURExpress Disulfide Bond Enhancer (NEB #E6820) and NEBExpress GamS Nuclease Inhibitor (NEB #P0774)


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FAQs for NEBExpress® Cell-free E. coli Protein Synthesis System
Protocols for NEBExpress® Cell-free E. coli Protein Synthesis System
Application Notes for NEBExpress® Cell-free E. coli Protein Synthesis System
Protein Expression Using the NEBExpress Cell-free E. coli Protein Synthesis System
50 µL reactions containing 250 ng template DNA were incubated at 37°C for 3 hours. 2 µL of each reaction were analyzed by SDS-PAGE using a 10–20% Tris-glycine gel. The red dot indicates the protein of interest. M = Unstained Protein Standard, Broad range (NEB #P7717), “Neg” = negative control, no DNA. 
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Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email [email protected].

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.


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