Protein Expression Products

New England Biolabs is committed to providing advanced tools for protein expression. As a leading supplier for reagents in the biotechnology field, NEB understands the importance of convenient and easy-to-use systems for high level expression and purification of proteins. We invite you to review our growing range of expression systems, including our new line of NEBExpress® products, resulting from our 40+ years of experience in cloning, over-expression and purification. We use these solutions in our own research and manufacturing processes, and know that quality and performance are critical – all of our products are stringently tested so that you can be sure they will work optimally for your solution, just as we rely on them to work in ours.

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Protein Expression

BL21 Competent E. coli

BL21(DE3) Competent E. coli

E. coli Ribosome

Factor Xa Protease

IMPACT™ Kit

K. lactis GG799 Competent Cells

K. lactis Protein Expression Kit

Lemo21(DE3) Competent E. coli

NEBExpress® Cell-free E. coli Protein Synthesis System

NEBExpress® Competent E. coli (High Efficiency)

NEBExpress® GamS Nuclease Inhibitor

NEBExpress® Iq Competent E. coli (High Efficiency)

NEBExpress® MBP Fusion and Purification System

NiCo21(DE3) Competent E. coli

pMAL-c6T Vector

pTXB1 Vector

pTYB21 Vector

PURExpress® Disulfide Bond Enhancer

PURExpress® In Vitro Protein Synthesis Kit

PURExpress® Δ (aa, tRNA) Kit

PURExpress® Δ RF123 Kit

PURExpress® Δ Ribosome Kit

RNase Inhibitor, Murine

SHuffle® Express Competent E. coli

SHuffle® T7 Competent E. coli

SHuffle® T7 Express Competent E. coli

SHuffle® T7 Express lysY Competent E. coli

T7 Express Competent E. coli (High Efficiency)

T7 Express lysY Competent E. coli (High Efficiency)

T7 Express lysY/Iq Competent E. coli (High Efficiency)

TEV Protease

Yeast Carbon Base Medium Powder

Cell-Free Protein Expression

E. coli Ribosome

NEBExpress® Cell-free E. coli Protein Synthesis System

NEBExpress® GamS Nuclease Inhibitor

PURExpress® Disulfide Bond Enhancer

PURExpress® In Vitro Protein Synthesis Kit

PURExpress® Δ (aa, tRNA) Kit

PURExpress® Δ RF123 Kit

PURExpress® Δ Ribosome Kit

RNase Inhibitor, Murine

Protein Expression in Yeast

K. lactis GG799 Competent Cells

K. lactis Protein Expression Kit

Yeast Carbon Base Medium Powder

Expression of Difficult Proteins

Factor Xa Protease

Lemo21(DE3) Competent E. coli

NEBExpress® MBP Fusion and Purification System

pMAL-c6T Vector

PURExpress® Disulfide Bond Enhancer

PURExpress® In Vitro Protein Synthesis Kit

SHuffle® Express Competent E. coli

SHuffle® T7 Competent E. coli

SHuffle® T7 Express Competent E. coli

SHuffle® T7 Express lysY Competent E. coli

T7 Express lysY Competent E. coli (High Efficiency)

T7 Express lysY/Iq Competent E. coli (High Efficiency)

TEV Protease

Protein Expression in E. Coli

BL21 Competent E. coli

BL21(DE3) Competent E. coli

IMPACT™ Kit

Lemo21(DE3) Competent E. coli

NEBExpress® Competent E. coli (High Efficiency)

NEBExpress® Iq Competent E. coli (High Efficiency)

NEBExpress® MBP Fusion and Purification System

NiCo21(DE3) Competent E. coli

pMAL-c6T Vector

pTXB1 Vector

pTYB21 Vector

SHuffle® Express Competent E. coli

SHuffle® T7 Competent E. coli

SHuffle® T7 Express Competent E. coli

SHuffle® T7 Express lysY Competent E. coli

T7 Express Competent E. coli (High Efficiency)

T7 Express lysY Competent E. coli (High Efficiency)

T7 Express lysY/Iq Competent E. coli (High Efficiency)

Cell-Free Protein Expression

E. coli Ribosome

NEBExpress® Cell-free E. coli Protein Synthesis System

NEBExpress® GamS Nuclease Inhibitor

PURExpress® Disulfide Bond Enhancer

PURExpress® In Vitro Protein Synthesis Kit

PURExpress® Δ (aa, tRNA) Kit

PURExpress® Δ RF123 Kit

PURExpress® Δ Ribosome Kit

RNase Inhibitor, Murine

NEBExpress® Cell-free E. coli Protein Synthesis System

NEBExpress® Cell-free E. coli Protein Synthesis System

NEBExpress® GamS Nuclease Inhibitor

PURExpress® Disulfide Bond Enhancer

RNase Inhibitor, Murine

PURExpress

E. coli Ribosome

PURExpress® Disulfide Bond Enhancer

PURExpress® In Vitro Protein Synthesis Kit

PURExpress® Δ (aa, tRNA) Kit

PURExpress® Δ RF123 Kit

PURExpress® Δ Ribosome Kit

RNase Inhibitor, Murine

Protein Expression in Yeast

K. lactis GG799 Competent Cells

K. lactis Protein Expression Kit

Yeast Carbon Base Medium Powder

Expression of Difficult Proteins

Lemo21(DE3) Competent E. coli

NEBExpress® MBP Fusion and Purification System

pMAL-c6T Vector

PURExpress® Disulfide Bond Enhancer

PURExpress® In Vitro Protein Synthesis Kit

SHuffle® Express Competent E. coli

SHuffle® T7 Competent E. coli

SHuffle® T7 Express Competent E. coli

SHuffle® T7 Express lysY Competent E. coli

T7 Express lysY Competent E. coli (High Efficiency)

T7 Express lysY/Iq Competent E. coli (High Efficiency)

Disulfide-bonded Protein Expression

PURExpress® Disulfide Bond Enhancer

PURExpress® In Vitro Protein Synthesis Kit

SHuffle® Express Competent E. coli

SHuffle® T7 Competent E. coli

SHuffle® T7 Express Competent E. coli

SHuffle® T7 Express lysY Competent E. coli

Membrane Protein Expression

Lemo21(DE3) Competent E. coli

T7 Express lysY/Iq Competent E. coli (High Efficiency)

Toxic Protein Expression

Lemo21(DE3) Competent E. coli

PURExpress® In Vitro Protein Synthesis Kit

T7 Express lysY Competent E. coli (High Efficiency)

T7 Express lysY/Iq Competent E. coli (High Efficiency)

Target Protein Insolubility

Factor Xa Protease

NEBExpress® MBP Fusion and Purification System

pMAL-c6T Vector

TEV Protease

Protein Expression in E. Coli

BL21 Competent E. coli

BL21(DE3) Competent E. coli

IMPACT™ Kit

Lemo21(DE3) Competent E. coli

NEBExpress® Competent E. coli (High Efficiency)

NEBExpress® Iq Competent E. coli (High Efficiency)

NEBExpress® MBP Fusion and Purification System

NiCo21(DE3) Competent E. coli

pMAL-c6T Vector

pTXB1 Vector

pTYB21 Vector

SHuffle® Express Competent E. coli

SHuffle® T7 Competent E. coli

SHuffle® T7 Express Competent E. coli

SHuffle® T7 Express lysY Competent E. coli

T7 Express Competent E. coli (High Efficiency)

T7 Express lysY Competent E. coli (High Efficiency)

T7 Express lysY/Iq Competent E. coli (High Efficiency)

T7 Expression

BL21(DE3) Competent E. coli

Lemo21(DE3) Competent E. coli

NiCo21(DE3) Competent E. coli

SHuffle® Express Competent E. coli

SHuffle® T7 Competent E. coli

SHuffle® T7 Express Competent E. coli

SHuffle® T7 Express lysY Competent E. coli

T7 Express Competent E. coli (High Efficiency)

T7 Express lysY Competent E. coli (High Efficiency)

T7 Express lysY/Iq Competent E. coli (High Efficiency)

Non-T7 Expression

BL21 Competent E. coli

NEBExpress® Competent E. coli (High Efficiency)

NEBExpress® Iq Competent E. coli (High Efficiency)

NEBExpress® MBP Fusion and Purification System

pMAL-c6T Vector