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RT-qPCR, RT-PCR and cDNA Synthesis

RNA can be detected, quantified and manipulated through copying via the use of reverse transcriptases (RTs), which are RNA-dependent DNA polymerases. RTs synthesize a strand of DNA that is complementary to the original RNA template and is referred to as cDNA. This cDNA can then be further amplified through PCR, qPCR, or isothermally using, for example, one-step RT-LAMP. Alternately, the cDNA can be used in library preparation for next generation sequencing.

New England Biolabs provides a broad variety of reverse transcriptases and kits for use in these experiments. In addition to stand alone enzymes such as ProtoScript II, NEB provides a number of optimized kits for RT-qPCR, RT-PCR, RNA Seq and isothermal RNA detection.


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Protocols for RT-qPCR, RT-PCR and cDNA Synthesis
    Publications related to RT-qPCR, RT-PCR and cDNA Synthesis
    • Wulf, M.G., Maguire, S., Humbert, P., Dai, N., Bei, Y., Nichols, N.M., Correa, I.R., Jr., Guan, S (2019) Non templated addition and template switching by Moloney murine leukemia virus MMLV based reverse transcriptases co occur and compete with each other J Biol Chem; 294(48), 18220-18231.. PubMedID: 31640989, DOI: 10.1074/jbc.RA119.010676
    • Potapov, V., Fu, X., Dai, N., Correa, I.R., Jr., Tanner, N.A., Ong, J.L (2018) Base modificatons affecting RNA polymerase and reverse transcriptase fidelity Nucleic Acids Res; 46(11): 5753-5763, PubMedID: 29750267
cDNA Synthesis Selection Chart
NEB offers several reagents for cDNA synthesis for use in applications including qPCR and qRT-PCR. For your convenience, reagents are available as kits or standalone products, depending on your needs.
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.


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