NEB research on ligase fidelity has led to the development of tools that aid in the design of high fidelity assemblies. These tools can suggest additional high fidelity overhangs.

NEBridge Ligase Fidelity Viewer®
Visualize overhang ligation preferences
NEBridge GetSet® Tool
Predict high-fidelity junction sets
NEBridge SplitSet® Tool
Split DNA sequence for scarless high-fidelity assembly