Home FAQs Can T4 RNA Ligase 1 ligate triphosphates?

FAQ: Can T4 RNA Ligase 1 ligate triphosphates?

No. However, the RNA can be treated first with CIP to remove the triphosphate, and then with a kinase, to add a single phosphate. Fragments containing these 5’ monophosphates can be ligated by T4 RNA ligase 1.