Home FAQs Is M-MuLV Reverse Transcriptase active at temperatures higher than 42°C?

FAQ: Is M-MuLV Reverse Transcriptase active at temperatures higher than 42°C?

For specific difficult templates (e.g. with high GC content or significant secondary structure) a higher reaction temperature may be used but the overall yield of the DNA may be decreased. We have successfully copied 6 kb messages by reverse transcription at 55°C.