New England Biolabs is working diligently to ensure we keep our employees and their families safe, while maintaining our business continuity. Despite a "Stay At Home" advisory being put in place in Massachusetts, USA, we are deemed an essential business, and our manufacturing and distribution teams continue to be fully operational. We are continuing to work with our suppliers and distribution partners to ensure uninterrupted access to our products and technical support.
HomeFAQsShould I replenish the SAM in my reaction?
FAQ: Should I replenish the SAM in my reaction?
SAM is unstable at pH 7.5, 37 °C. For extended reactions adding more SAM after 4 hours can improve results. Methylation reactions, however, are also greatly affected by S-adenosylhomocysteine. S-adenosylhomocysteine, the byproduct of the methylation reaction binds more tightly to methylases than does SAM. Inhibition by S-adenosylhomocysteine greatly reduces the reaction rate as time passes. Using more enzyme in shorter reactions may improve methylation.
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