Home FAQs What should my primer concentration be when using Phusion® High-Fidelity DNA Polymerase/Phusion® High-Fidelity PCR Master Mix?

FAQ: What should my primer concentration be when using Phusion® High-Fidelity DNA Polymerase/Phusion® High-Fidelity PCR Master Mix?

Primer concentrations should be between 200 nM and 1 µM, however 500 nM is typically optimal.

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