Home FAQs Why are there low molecular weight discrete bands on an agarose gel after a PCR using Phusion® High-Fidelity PCR Master Mix?

FAQ: Why are there low molecular weight discrete bands on an agarose gel after a PCR using Phusion® High-Fidelity PCR Master Mix?

Non-specific products are being amplified. To avoid this we suggest the following:
* Raise the annealing temperature.
* Shorten extension time.
* Titrate template amount.
* Lower primer concentration.